Academic Journal
Synthesis and characterization of chitosan nanoparticles and their inhibitory effects against Streptococcus mutans: role of concentration and exposure time.
| Τίτλος: | Synthesis and characterization of chitosan nanoparticles and their inhibitory effects against Streptococcus mutans: role of concentration and exposure time. |
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| Συγγραφείς: | Costa BP; Graduate Program in Dentistry, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Castelo Branco III, João Pessoa, PB, 58051-900, Brazil., Reis RM; Departamento de Saúde Coletiva, Odontopediatria E Ortodontia, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (FOP/UNICAMP), Av. Limeira, 901, Areião, Piracicaba, SP, 13414-903, Brazil., de Castro Bernardino K; Department of Pediatric Dentistry-Dental School, Universidade Federal de Uberlândia-Campus Umuarama, Bloco UMU2G. Av. Pará, 1720, Umuarama, Uberlândia, MG, 38405-320, Brazil., Aguiar RT; Graduate Program in Dentistry, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Castelo Branco III, João Pessoa, PB, 58051-900, Brazil., Carlo HL; Department of Pediatric Dentistry-Dental School, Universidade Federal de Uberlândia-Campus Umuarama, Bloco UMU2G. Av. Pará, 1720, Umuarama, Uberlândia, MG, 38405-320, Brazil., Dos Santos RL; Department of Dentistry, Universidade Federal de Juiz de Fora, Avenida Doutor Raimundo Rezende N. 330, Centro, Governador Valadares, Juiz de Fora, MG, 35012-140, Brazil., de Carvalho FG; Department of Pediatric Dentistry-Dental School, Universidade Federal de Uberlândia-Campus Umuarama, Bloco UMU2G. Av. Pará, 1720, Umuarama, Uberlândia, MG, 38405-320, Brazil. fabiola.carlo@ufu.br. |
| Πηγή: | Odontology [Odontology] 2026 Oct; Vol. 114 (4), pp. 1955-1965. Date of Electronic Publication: 2025 Nov 12. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer-Verlag Tokyo Country of Publication: Japan NLM ID: 101134822 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1255 (Electronic) Linking ISSN: 16181247 NLM ISO Abbreviation: Odontology Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Tokyo : Springer-Verlag Tokyo, c2001- |
| Ιατρικοί όροι (MeSH): | Streptococcus mutans*/drug effects , Chitosan*/pharmacology , Chitosan*/chemical synthesis , Chitosan*/chemistry , Nanoparticles*/chemistry , Anti-Bacterial Agents*/pharmacology, Biofilms/drug effects ; Bacterial Adhesion/drug effects ; Microscopy, Electron, Transmission ; Spectroscopy, Fourier Transform Infrared ; Microbial Sensitivity Tests ; Time Factors |
| Περίληψη: | This study aimed to synthesize and characterize chitosan nanoparticles suspension (ChNPs) and to evaluate their inhibitory effects on Streptococcus mutans (S. mutans) adherence and biofilm formation under different concentrations and exposure times. ChNPs were synthesized via ionic gelation using tripolyphosphate (TPP) and characterized by Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering. Antibacterial activity was investigated through inhibition zone tests, as well as minimum inhibitory and bactericidal concentration (MIC and MBC) assays. ChNPs at MIC, 2 × MIC, and 4 × MIC were tested against S. mutans, alongside 0.12% chlorhexidine (positive control) and 0.9% saline solution (negative control). In adherence and biofilm formation assays, solutions were applied for 2 h and 60 s, respectively. For mature biofilms, treatments were applied for 60 s every 24 h or continuously for 24 or 48 h. Inhibition percentage (%I) was quantified using crystal violet staining. Data were analyzed by one-way ANOVA and Tukey's test (α = 0.05). TEM images showed small spherical particles with diameter and zeta potential values of 75.8 nm and + 46.2 mV, respectively. MIC and MBC were 550,0 and 275,0 µg/mL, respectively. ChNPs inhibited 40% of bacterial adherence at all concentrations (p = 0.06). Mature biofilm inhibition ranged from 90 to 100% after continuous exposure for 24 or 48 h (p = 0.251). ChNPs at all concentrations and CHX 0.12% exhibited high %I (90-100%). These findings demonstrate that ChNPs effectively reduced S. mutans adherence and disrupted biofilm formation, with greater efficacy achieved through prolonged contact, highlighting their potential as bioactive agents for preventing or controlling cariogenic biofilms in oral healthcare. (© 2025. The Author(s), under exclusive licence to The Society of The Nippon Dental University.) |
| Competing Interests: | Declarations. Conflict of interest: All authors declare that there is no conflict of interest regarding the publication of this article. |
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| Grant Information: | 458158/2014-3 Conselho Nacional de Desenvolvimento Científico e Tecnológico; APQ-02895-21 Fundação de Amparo à Pesquisa do Estado de Minas Gerais; CDS/PPM-00299-16 Fundação de Amparo à Pesquisa do Estado de Minas Gerais; FAPEMIG RED-00204-23 Fundação de Amparo à Pesquisa do Estado de Minas Gerais; 406840/2022-9 INCT Saúde Oral e Odontologia |
| Contributed Indexing: | Keywords: Streptococcus mutans; Biofilms; Chitosan; Nanoparticles |
| Substance Nomenclature: | 9012-76-4 (Chitosan) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20251112 Date Completed: 20261002 Latest Revision: 20261008 |
| Update Code: | 20261008 |
| DOI: | 10.1007/s10266-025-01250-x |
| PMID: | 41222863 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1618-1255 |
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| DOI: | 10.1007/s10266-025-01250-x |