Academic Journal
Therapeutic potential of endothelial progenitor cell-derived exosomal miRNA-31 in alleviating septic peritonitis via E-selectin inhibition.
| Τίτλος: | Therapeutic potential of endothelial progenitor cell-derived exosomal miRNA-31 in alleviating septic peritonitis via E-selectin inhibition. |
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| Συγγραφείς: | Liu S; Department of Emergency, Gansu Provincial Hospital, Lanzhou, Gansu Province, China., Wang B; The First Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, Gansu Province, China., Zhang M; The First Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, Gansu Province, China., Ma G; The First Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, Gansu Province, China. |
| Πηγή: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2026 Jan 21; Vol. 215 (1). |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Association of Immunologists Country of Publication: England NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| Ιατρικοί όροι (MeSH): | MicroRNAs*/genetics , MicroRNAs*/metabolism , MicroRNAs*/blood , Peritonitis*/therapy , Peritonitis*/immunology , Sepsis*/immunology , Sepsis*/therapy , Endothelial Progenitor Cells*/metabolism , Exosomes*/metabolism , Exosomes*/genetics , E-Selectin*/antagonists & inhibitors , E-Selectin*/metabolism, Animals ; Humans ; Mice ; Male ; Female ; Disease Models, Animal ; Apoptosis ; Middle Aged ; Mice, Inbred C57BL ; Cell Proliferation ; Aged |
| Περίληψη: | Septic peritonitis (SP) is a severe infectious complication associated with high morbidity and mortality. The role of microRNAs, particularly endothelial progenitor cell-derived exosomal miRNA-31 (EPC-ExosmiR-31), in modulating inflammatory pathways in SP has garnered scholars' attention. However, its precise role and mechanism of action remain inadequately explored. Serum levels of miRNA-31 (miR-31) and soluble E-selectin (sSELE) were measured in patients with SP and healthy controls using qRT-PCR and ELISA. A colon ascendens stent peritonitis (CASP) mouse model was employed to replicate SP, and an in vitro sepsis model was induced via lipopolysaccharide (LPS) stimulation of mouse venous endothelial cells. The effects of EPC-ExosmiR-31 on cellular proliferation, apoptosis, and inflammatory markers were assessed through Giemsa and hematoxylin and eosin staining, qRT-PCR, Western blotting, and dual-luciferase reporter assay. Statistical analysis was conducted to evaluate the correlation among miR-31, sSELE, and sepsis severity. Patients with SP exhibited significantly lower serum miR-31 levels and elevated sSELE levels compared with healthy controls, and these changes were correlated with the severity of sepsis. In the CASP mouse model, extensive neutrophilic infiltration and inflammatory tissue damage were found in the lungs, liver, and colon. EPC-ExosmiR-31 administration significantly mitigated these effects by reducing SELE, caspase-3, and Bax expression levels, while increasing Bcl-2 expression level. In vitro, miR-31 enhanced cell proliferation and inhibited apoptosis in LPS-treated endothelial cells. The dual-luciferase reporter assay confirmed that miR-31 could directly target SELE. EPC-ExosmiR-31 alleviated SP by targeting SELE, thereby reducing endothelial dysfunction and systemic inflammation, reflecting a promising therapeutic approach for managing SP. (© The Author(s) 2025. Published by Oxford University Press on behalf of The American Association of Immunologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| Grant Information: | 22JR5RA674 Natural Science Foundation of Gansu Province; 22JSSYD-46 Intramural Research of Gansu Provincial Hospital; 23GSSYD-11 In-House Research Foundation of Gansu Provincial People's Hospital; 25JRRA1214 Joint Research Foundation of Gansu Province |
| Contributed Indexing: | Keywords: E-selectin; endothelial progenitor cells; exosomes; miRNA-31; septic peritonitis |
| Substance Nomenclature: | 0 (MicroRNAs) 0 (MIRN31 microRNA, human) 0 (E-Selectin) 0 (Mirn31 microRNA, mouse) |
| Entry Date(s): | Date Created: 20251030 Date Completed: 20260130 Latest Revision: 20260918 |
| Update Code: | 20260918 |
| DOI: | 10.1093/jimmun/vkaf271 |
| PMID: | 41166204 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1550-6606 |
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| DOI: | 10.1093/jimmun/vkaf271 |