Academic Journal
Comparative Performance Analysis of Corneal Characteristics During Extended Hypothermic Storage in XTRA4 and Optisol-GS.
| Τίτλος: | Comparative Performance Analysis of Corneal Characteristics During Extended Hypothermic Storage in XTRA4 and Optisol-GS. |
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| Συγγραφείς: | Ellison MS; VisionGift, Portland, OR; and., Straiko MMW; VisionGift, Portland, OR; and., Ragazzi E; Studium Patavinum, University of Padova, Padova, Italy., Chamberlain DZ; VisionGift, Portland, OR; and., Tran KD; VisionGift, Portland, OR; and. |
| Πηγή: | Cornea [Cornea] 2026 Mar 01; Vol. 45 (3), pp. 351-360. Date of Electronic Publication: 2025 Oct 02. |
| Τύπος έκδοσης: | Journal Article; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 8216186 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1536-4798 (Electronic) Linking ISSN: 02773740 NLM ISO Abbreviation: Cornea Subsets: MEDLINE |
| Imprint Name(s): | Publication: Hagerstown, MD : Lippincott Williams & Wilkins Original Publication: New York, N.Y. : Masson Pub. USA, c1982- |
| Ιατρικοί όροι (MeSH): | Endothelium, Corneal*/cytology , Organ Preservation*/methods , Chondroitin Sulfates*/pharmacology , Cryopreservation*/methods , Complex Mixtures*/pharmacology , Gentamicins*/pharmacology , Cornea*, Humans ; Tomography, Optical Coherence ; Male ; Middle Aged ; Tissue Donors ; Female ; Aged ; Cell Count ; Drug Combinations ; Cell Survival ; Corneal Transplantation ; Adult ; Culture Media, Serum-Free ; Eye Banks ; Dextrans |
| Περίληψη: | Purpose: To compare endothelial cell morphometry, corneal thickness, and transparency of donor corneas during extended hypothermic storage in XTRA4 and Optisol-GS. Methods: Mate donor corneas were stored in XTRA4 or Optisol-GS at 2 to 8°C for up to 28 days. Endothelial cell density, coefficient of variation (CV), and hexagonality (HEX) were evaluated by specular microscopy. Central corneal thickness (CCT) was measured by Optical Coherence Tomography (OCT). Endothelium quality, stromal edema, and Descemet folds were graded by slit lamp using defined grading scales. Results: Endothelial cell density, CV, and HEX were similar between cohorts through day 21 ( P > 0.05); however, HEX was higher in the XTRA4 group on the final day ( P = 0.03). Endothelial cell viability was comparable at all time points ( P > 0.05). Initial stromal thickness averaged 520 ± 38 μm (XTRA4) and 502 ± 28 μm (Optisol-GS), with no difference through day 7 ( P > 0.05). Beyond day 7, XTRA4 corneas were significantly thinner (all P < 0.05). Stromal thickness increased over time in both cohorts ( P < 0.05), with a smaller Δ in XTRA4 (15 ± 19 μm) versus Optisol-GS (158 ± 54 μm). Edema scores were similar at baseline (median = 1), although Optisol-GS trended higher by day 21 ( P < 0.001). Descemet folds were fewer in Optisol-GS initially by 1 grade ( P < 0.01) but became more severe than XTRA4 after day 14 (all P < 0.01). Conclusions: XTRA4 preserves the corneal endothelium similarly to Optisol-GS for up to 14 days while maintaining consistently thinner corneas for up to 28 days. This antiswelling property may improve eye bank logistics and support future laboratory and clinical studies toward extending hypothermic storage beyond the current 14-day limit. (Copyright © 2025 Wolters Kluwer Health, Inc. All rights reserved.) |
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| Grant Information: | High Impact Grant Eye Bank Association of America; Partial matching funds AL.CHI.MI.A S.R.L. |
| Contributed Indexing: | Keywords: corneal clarity; corneal storage solution; corneal swelling; endothelia cell density; extended hypothermic storage; hypothermic storage solution |
| Substance Nomenclature: | 97794-22-4 (Optisol) 9007-28-7 (Chondroitin Sulfates) 0 (Drug Combinations) 0 (Complex Mixtures) 0 (Gentamicins) 0 (Culture Media, Serum-Free) 0 (Dextrans) |
| Entry Date(s): | Date Created: 20251017 Date Completed: 20260204 Latest Revision: 20260212 |
| Update Code: | 20260213 |
| DOI: | 10.1097/ICO.0000000000004003 |
| PMID: | 41105831 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1536-4798 |
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| DOI: | 10.1097/ICO.0000000000004003 |