Academic Journal
In Vitro Antibacterial Activity of Raw Honey From Apis mellifera, Awi Zone, Amhara, Ethiopia.
| Τίτλος: | In Vitro Antibacterial Activity of Raw Honey From Apis mellifera, Awi Zone, Amhara, Ethiopia. |
|---|---|
| Συγγραφείς: | Kumera B; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Gebreamanule B; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Atnkut B; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Merid M; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Tilahun T; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Tsega A; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Fassil A; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.; Doctoral School of Biology, Hungarian University of Agricultural and Life Sciences, Gödöllö, Hungary., Dessie Y; Department of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia., Kassie B; Department of Chemistry, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia. |
| Πηγή: | MicrobiologyOpen [Microbiologyopen] 2025 Oct; Vol. 14 (5), pp. e70076. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101588314 Publication Model: Print Cited Medium: Internet ISSN: 2045-8827 (Electronic) Linking ISSN: 20458827 NLM ISO Abbreviation: Microbiologyopen Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford : Wiley-Blackwell |
| Ιατρικοί όροι (MeSH): | Honey*/analysis , Anti-Bacterial Agents*/pharmacology , Bacteria*/drug effects, Escherichia coli/drug effects ; Klebsiella pneumoniae/drug effects ; Pseudomonas aeruginosa/drug effects ; Bees ; Animals ; Ethiopia ; Microbial Sensitivity Tests ; Hydrogen-Ion Concentration |
| Περίληψη: | Honey has been used for the treatment of various illnesses since time immemorial. Honey is employed as a remedy for the treatment of burns, microbial infections, cardiovascular disorders, and gastrointestinal problems. The objectives of this study were to evaluate physicochemical characteristics and in vitro antibacterial properties of honey produced by Apis mellifera against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Klebsiella pneumoniae. Honey samples were collected from three districts of the Awi zone from April to June 2023. The pH and moisture of collected honey were determined following the standard procedure. An antimicrobial susceptibility test of honey samples was performed using an agar well diffusion assay. The moisture contents and pH of collected honey samples from the three study sites were 13 ± 0.07-20 ± 0.09 and 3.76-4.01, respectively. There was no significant (p > 0.05) difference in pH between honey samples collected from the three sites. Similarly, there was no significant statistical association among the moisture content of honey samples (p > 0.05). Pure 100% honey samples showed the greatest inhibition zone against E. coli (19.20 mm) and the lowest against P. aeruginosa (2.4 mm). Honey concentration of 25% showed the lowest inhibition zone against all tested bacterial pathogens. Antibacterial effects of honey solutions decreased upon serial dilution from 100% to 25%. Our finding suggests that raw honey can be used as therapeutic agents against bacterial disease. (© 2025 The Author(s). MicrobiologyOpen published by John Wiley & Sons Ltd.) |
| References: | Int J Food Sci. 2019 Mar 4;2019:4101695. (PMID: 30949493) Arch Med Res. 2005 Sep-Oct;36(5):464-7. (PMID: 16099322) J Med Food. 2005 Spring;8(1):100-3. (PMID: 15857217) Sci Rep. 2018 Jun 13;8(1):9061. (PMID: 29899462) Int J Microbiol. 2019 Jun 2;2019:2464507. (PMID: 31281362) BMC Complement Med Ther. 2020 Mar 17;20(1):85. (PMID: 32178659) Microbiologyopen. 2025 Oct;14(5):e70076. (PMID: 41105045) Int J Microbiol. 2019 Apr 03;2019:7686130. (PMID: 31073310) Antibiotics (Basel). 2019 Dec 05;8(4):. (PMID: 31817375) BMC Res Notes. 2017 Nov 6;10(1):566. (PMID: 29110688) Am J Ther. 2014 Jul-Aug;21(4):304-23. (PMID: 23782759) Molecules. 2013 Apr 11;18(4):4233-46. (PMID: 23579991) Saudi J Biol Sci. 2022 Feb;29(2):730-734. (PMID: 35197738) IUBMB Life. 2012 Jan;64(1):48-55. (PMID: 22095907) Br J Oral Maxillofac Surg. 2008 Jan;46(1):55-6. (PMID: 17113690) Heliyon. 2023 Oct 09;9(10):e20830. (PMID: 37860501) Dermatol Res Pract. 2018 Jun 19;2018:7021713. (PMID: 30018636) Asian Pac J Trop Biomed. 2014 Aug;4(8):633-41. (PMID: 25183333) Food Chem. 2020 Dec 1;332:127229. (PMID: 32688187) |
| Grant Information: | The authors received no specific funding for this work. |
| Contributed Indexing: | Keywords: agar well diffusion assay; antibacterial activity; honey; pathogen |
| Substance Nomenclature: | 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20251017 Date Completed: 20251017 Latest Revision: 20260925 |
| Update Code: | 20260925 |
| PubMed Central ID: | PMC12532491 |
| DOI: | 10.1002/mbo3.70076 |
| PMID: | 41105045 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2045-8827 |
|---|---|
| DOI: | 10.1002/mbo3.70076 |