Academic Journal
Investigation of shockwave treatment for disruption of bacterial biofilm on tubular structure.
| Τίτλος: | Investigation of shockwave treatment for disruption of bacterial biofilm on tubular structure. |
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| Συγγραφείς: | Jo J; Industry 4.0 Convergence Bionics Engineering, Department of Biomedical Engineering, Pukyong National University, Busan, 48513, Republic of Korea.; Research Center for Marine-Integrated Biomedical Technology, The National Key Research Institutes in Universities, Pukyong National University, Busan, 48513, Republic of Korea., Stadler S; Johnson & Johnson Med Tech, New Brunswick, NJ, USA., Costandi P; Johnson & Johnson Med Tech, New Brunswick, NJ, USA., Hasenberg T; Johnson & Johnson Med Tech, New Brunswick, NJ, USA., Kang HW; Industry 4.0 Convergence Bionics Engineering, Department of Biomedical Engineering, Pukyong National University, Busan, 48513, Republic of Korea. wkang@pukyong.ac.kr.; Research Center for Marine-Integrated Biomedical Technology, The National Key Research Institutes in Universities, Pukyong National University, Busan, 48513, Republic of Korea. wkang@pukyong.ac.kr.; Major of Biomedical Engineering, Division of Smart Healthcare and Digital Healthcare Research Center, College of Information Technology and Convergence, Pukyong National University, 45 Yongso-ro Nam-gu, Busan, 48513, Republic of Korea. wkang@pukyong.ac.kr. |
| Πηγή: | Scientific reports [Sci Rep] 2025 Oct 15; Vol. 15 (1), pp. 36041. Date of Electronic Publication: 2025 Oct 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Ιατρικοί όροι (MeSH): | Biofilms*/drug effects , Biofilms*/radiation effects , Biofilms*/growth & development , Pseudomonas aeruginosa*/drug effects , Pseudomonas aeruginosa*/physiology , Pseudomonas aeruginosa*/radiation effects , Anti-Bacterial Agents*/pharmacology , High-Energy Shock Waves*, Microbial Viability/drug effects ; Microbial Viability/radiation effects ; Ciprofloxacin/pharmacology ; Microscopy, Electron, Scanning ; Microscopy, Confocal |
| Περίληψη: | Biofilms, which are structures formed by microorganisms, are protected by extracellular polymeric substances (EPS) secreted by bacteria against external threats, including antibiotics. The current study aims to assess the effects of shockwave treatment combined with antibiotic therapy on Pseudomonas aeruginosa biofilms in tubular structures in vitro. The biofilms were formed on the inner surfaces of silicone tubes for three days under dynamic conditions. The biofilms were treated with shockwave treatment (120 pulses at 2 Hz), followed by exposure to 4 µg/ml ciprofloxacin for 6 h. Bacterial viability was assessed using colony-forming unit (CFU) and confocal laser scanning microscopy (CLSM) with SYTO9/PI staining, while biofilm detachment was evaluated via crystal violet (CV) staining and scanning electron microscopy (SEM). According to the SEM and CFU analysis, the shockwave and antibiotic-combined treatment significantly detached the biofilm, removing up to 97.5% of the surface area and decreased bacterial viability by 40%, compared to untreated control biofilms. The CV staining showed a significant reduction in biofilm biomass to an OD (© 2025. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Grant Information: | RS-2021-NR060118 Ministry of Education |
| Contributed Indexing: | Keywords: Pseudomonas aeruginosa; Antibiotic efficacy; Balloon catheter; Biofilm; Shockwave treatment |
| Substance Nomenclature: | 0 (Anti-Bacterial Agents) 5E8K9I0O4U (Ciprofloxacin) |
| Entry Date(s): | Date Created: 20251015 Date Completed: 20251015 Latest Revision: 20251019 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12528757 |
| DOI: | 10.1038/s41598-025-19955-y |
| PMID: | 41094089 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2045-2322 |
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| DOI: | 10.1038/s41598-025-19955-y |