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FOF1-ATPase mediates regulation of fermentation and energy metabolism at pH 5.5.

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Τίτλος: FOF1-ATPase mediates regulation of fermentation and energy metabolism at pH 5.5.
Συγγραφείς: Gevorgyan H; Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 0025, Yerevan, Armenia.; Scientific-Research Institute of Biology, Faculty of Biology, Yerevan State University, 1 A. Manoogian Str., 0025, Yerevan, Armenia.; Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, 0025, Yerevan, Armenia., Trchounian K; Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 0025, Yerevan, Armenia. k.trchounian@ysu.am.; Scientific-Research Institute of Biology, Faculty of Biology, Yerevan State University, 1 A. Manoogian Str., 0025, Yerevan, Armenia. k.trchounian@ysu.am.; Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, 0025, Yerevan, Armenia. k.trchounian@ysu.am.
Πηγή: Scientific reports [Sci Rep] 2025 Oct 14; Vol. 15 (1), pp. 35873. Date of Electronic Publication: 2025 Oct 14.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Imprint Name(s): Original Publication: London : Nature Publishing Group, copyright 2011-
Ιατρικοί όροι (MeSH): Escherichia coli*/metabolism , Escherichia coli*/enzymology , Escherichia coli*/genetics , Proton-Translocating ATPases*/metabolism , Proton-Translocating ATPases*/genetics , Escherichia coli Proteins*/metabolism , Escherichia coli Proteins*/genetics , Fermentation* , Energy Metabolism*, Glucose/metabolism ; Formates/metabolism ; Glycerol/metabolism ; Hydrogen/metabolism ; Hydrogen-Ion Concentration ; Proton-Motive Force
Περίληψη: The activity of FOF1-ATP synthase (FOF1) is vital for the survival and anaerobic growth under acidic conditions providing efficient metabolism and energy for cellular function. This study demonstrated the role of FOF1-ATPase in the metabolic pathways and regulation of proton motive force in Escherichia coli at pH 5.5 during the fermentation of glucose, glycerol and formate. Alteration of the ratio of fermentation end-products was shown. The possible mechanisms for efficient energy utilization in cells lacking FOF1 were shown. It is suggested that Fdh-H transfers H+ from formate to Hyd, which serves not only for H₂ generation but also for H+ cycling to FOF1 or directly to FOF1 in cells grown for 20 h. The interaction between H2 and H+ cycling was proposed as an essential mechanism for balancing ΔpH at acidic conditions. FOF1-ATPase was identified to play a pivotal role in forming both components of Δp at 20 h and 72 h.
(© 2025. The Author(s).)
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
References: J Bacteriol. 2000 Feb;182(3):620-6. (PMID: 10633094)
Biotechnol Bioeng. 2006 Aug 5;94(5):821-9. (PMID: 16715533)
Biol Pharm Bull. 2022;45(10):1404-1411. (PMID: 36184496)
Biochim Biophys Acta Bioenerg. 2023 Jan 1;1864(1):148919. (PMID: 36152681)
Microb Biotechnol. 2021 May;14(3):829-858. (PMID: 33438829)
Biosci Rep. 2000 Aug;20(4):277-88. (PMID: 11092250)
J Bacteriol. 1970 Mar;101(3):885-91. (PMID: 4908786)
Curr Microbiol. 2013 Jan;66(1):49-55. (PMID: 23053487)
Acta Physiol Scand. 1978 May;103(1):40-6. (PMID: 665258)
FEMS Microbiol Lett. 2022 Nov 25;369(1):. (PMID: 36370455)
J Bioenerg Biomembr. 2011 Dec;43(6):645-50. (PMID: 22081210)
FEBS Lett. 1999 Mar 26;447(2-3):144-8. (PMID: 10214935)
Microbiology (Reading). 2016 Sep;162(9):1672-1679. (PMID: 27488847)
Genes (Basel). 2020 Aug 25;11(9):. (PMID: 32854287)
Trends Biochem Sci. 2019 May;44(5):391-400. (PMID: 30655166)
Annu Rev Microbiol. 2013;67:65-81. (PMID: 23701194)
Nat Commun. 2020 Mar 20;11(1):1496. (PMID: 32198415)
PLoS One. 2010 Apr 12;5(4):e10132. (PMID: 20405029)
Cell Biochem Biophys. 2006;46(3):201-8. (PMID: 17272848)
Eur J Biochem. 1999 Jun;262(2):595-9. (PMID: 10336647)
FEMS Microbiol Rev. 2022 Nov 2;46(6):. (PMID: 35906711)
FEBS Lett. 2002 Apr 10;516(1-3):172-8. (PMID: 11959127)
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3896-900. (PMID: 4279406)
Arch Biochem Biophys. 2024 May;755:109999. (PMID: 38621444)
Biophys Chem. 2005 May 1;115(1):55-61. (PMID: 15848284)
PLoS One. 2012;7(12):e52577. (PMID: 23300708)
J Bacteriol. 1974 Mar;117(3):1141-8. (PMID: 4205190)
IUBMB Life. 2020 May;72(5):915-921. (PMID: 31856407)
Anal Biochem. 1983 Apr 1;130(1):54-9. (PMID: 6346946)
IUBMB Life. 2021 Jun;73(6):883-892. (PMID: 33773019)
Biochim Biophys Acta Bioenerg. 2024 Apr 1;1865(2):149034. (PMID: 38354879)
Biochim Biophys Acta. 2012 Oct;1817(10):1711-21. (PMID: 22459334)
Biochimie. 2025 Sep;236:1-9. (PMID: 40516687)
IUBMB Life. 2018 Oct;70(10):1040-1047. (PMID: 30161297)
FEMS Microbiol Rev. 2014 Nov;38(6):1091-125. (PMID: 24898062)
Pharmacol Rev. 1972 Mar;24(1):67-157. (PMID: 4402043)
Microorganisms. 2024 Feb 01;12(2):. (PMID: 38399713)
Mol Microbiol. 1991 Nov;5(11):2807-14. (PMID: 1779767)
Adv Microb Physiol. 2009;55:1-79, 317. (PMID: 19573695)
J Exp Biol. 2000 Jan;203(Pt 1):89-95. (PMID: 10600677)
J Bacteriol. 2011 Jun;193(12):3072-7. (PMID: 21478347)
FEMS Microbiol Lett. 2001 Oct 16;204(1):61-4. (PMID: 11682179)
FEBS Lett. 1974 Jul 15;43(2):189-94. (PMID: 4277328)
J Biol Chem. 2010 Jun 4;285(23):17498-506. (PMID: 20299454)
Bacteriol Rev. 1977 Mar;41(1):100-80. (PMID: 860983)
J Bacteriol. 1991 Jan;173(2):687-96. (PMID: 1987159)
J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3. (PMID: 159458)
World J Microbiol Biotechnol. 2010 Jul;26(7):1181-6. (PMID: 24026921)
J Bacteriol. 1984 Dec;160(3):1055-60. (PMID: 6238948)
Grant Information: 23LCG-1F003 State Committee of Science
Contributed Indexing: Keywords: Acidic pH; FOF1-ATPase; Proton and hydrogen cycling; Proton conductance; Proton motive force; Proton permeability
Substance Nomenclature: EC 3.6.3.14 (Proton-Translocating ATPases)
IY9XDZ35W2 (Glucose)
0 (Formates)
PDC6A3C0OX (Glycerol)
0YIW783RG1 (formic acid)
7YNJ3PO35Z (Hydrogen)
0 (Escherichia coli Proteins)
Entry Date(s): Date Created: 20251014 Date Completed: 20251014 Latest Revision: 20251017
Update Code: 20260130
PubMed Central ID: PMC12521522
DOI: 10.1038/s41598-025-21047-w
PMID: 41087635
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2045-2322
DOI:10.1038/s41598-025-21047-w