Academic Journal
Best practices in mouse models of skin transplantation.
| Τίτλος: | Best practices in mouse models of skin transplantation. |
|---|---|
| Συγγραφείς: | Borges TJ; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Krummey SM; Department of Pathology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA., Ford ML; Department of Surgery and Emory Transplant Center, Emory University, Atlanta, Georgia, USA. Electronic address: mandy.ford@emory.edu. |
| Πηγή: | American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons [Am J Transplant] 2026 Mar; Vol. 26 (3), pp. 439-448. Date of Electronic Publication: 2025 Oct 10. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 100968638 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-6143 (Electronic) Linking ISSN: 16006135 NLM ISO Abbreviation: Am J Transplant Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2023- : [New York] : Elsevier Original Publication: Copenhagen : Munksgaard International Publishers, 2001- |
| Ιατρικοί όροι (MeSH): | Skin Transplantation*/methods , Graft Rejection*/immunology , Graft Rejection*/etiology , Graft Rejection*/prevention & control , Disease Models, Animal*, Graft Survival/immunology ; Animals ; Mice ; Models, Animal ; Humans |
| Περίληψη: | The murine skin transplant model has been used for decades as a sensitive in vivo model in which to dissect mechanistic aspects of alloreactivity. Indeed, seminal work defining the nature of alloreactivity was accomplished in the murine skin graft model. In the modern era, the incorporation of genetically altered animals, pharmacologic manipulation, and multiomics approaches in the murine skin graft model has significantly increased the mechanistic insight that can be gleaned from these studies. Because skin grafts are not primarily vascularized, the immune response against them is dominated by a CD8+ T cell response, with a lesser but important contribution of alloreactive CD4+ T cells. In contrast, donor-reactive antibody plays a lesser role in mediating skin transplant rejection. Importantly, the results of pharmacologic inhibition of T cell-directed novel targets for immunosuppression in transplantation in the murine skin graft model have largely reflected the results subsequently obtained in nonhuman primate renal transplant studies, demonstrating the physiologic relevance of the model. In this minireview, we further discuss the relative benefits and limitations of murine skin transplantation as a model of alloreactivity. (Copyright © 2025 American Society of Transplantation & American Society of Transplant Surgeons. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors of this manuscript have conflicts of interest to disclose as described by American Journal of Transplantation. Mandy L. Ford reports a relationship with Eledon that includes: consulting or advisory. Mandy L. Ford reports a relationship with Sanofi that includes: consulting or advisory. Mandy L. Ford reports a relationship with Veloxis Pharmaceuticals Inc that includes: consulting or advisory. The other authors of this manuscript have no conflicts of interest to disclose as described by American Journal of Transplantation. |
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| Grant Information: | R01 AI152516 United States AI NIAID NIH HHS; R01 AI164716 United States AI NIAID NIH HHS |
| Contributed Indexing: | Keywords: CD4(+) T lymphocyte; CD8(+) T lymphocyte; T cell-mediated rejection; mouse model; skin transplantation |
| Entry Date(s): | Date Created: 20251012 Date Completed: 20260307 Latest Revision: 20260919 |
| Update Code: | 20260919 |
| PubMed Central ID: | PMC12697377 |
| DOI: | 10.1016/j.ajt.2025.09.025 |
| PMID: | 41077307 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1600-6143 |
|---|---|
| DOI: | 10.1016/j.ajt.2025.09.025 |