Academic Journal
Immobilized pigeon pea urease on chitosan-PEG biocomposite for concurrent urea sensing in milk and blood samples.
| Τίτλος: | Immobilized pigeon pea urease on chitosan-PEG biocomposite for concurrent urea sensing in milk and blood samples. |
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| Συγγραφείς: | Kumar P; School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India., Ghosal A; School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India., Kayastha AM; School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: kayasthabhu@gmail.com. |
| Πηγή: | Food chemistry [Food Chem] 2025 Dec 15; Vol. 495 (Pt 3), pp. 146509. Date of Electronic Publication: 2025 Sep 25. |
| Τύπος έκδοσης: | Journal Article; Evaluation Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
| Ιατρικοί όροι (MeSH): | Urease*/chemistry , Milk*/chemistry , Urea*/blood , Urea*/analysis , Cajanus*/enzymology , Cajanus*/chemistry , Plant Proteins*/chemistry , Biosensing Techniques*/methods , Biosensing Techniques*/instrumentation, Chitosan/chemistry ; Enzymes, Immobilized/chemistry ; Polyethylene Glycols/chemistry ; Animals ; Cattle ; Kinetics |
| Περίληψη: | The ubiquitous hydrolytic enzyme urease converts urea into ammonia and carbon dioxide, making it an essential indicator for dairy product quality and kidney health. We developed a biocomposite sensor to meet this diagnostic necessity. This innovation strategically incorporates pigeon pea seed urease into chitosan-PEG. We verified our predicted model with a 91.30 % immobilization efficiency via our thorough process. The immobilized urease showed strong catalytic activity, with a V (Copyright © 2025 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Biocomposite; Chitosan; Milk urea; Pigeon pea; Polyethylene glycol; Urease |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 9012-76-4 (Chitosan) 8W8T17847W (Urea) 0 (Enzymes, Immobilized) 3WJQ0SDW1A (Polyethylene Glycols) 0 (Plant Proteins) |
| Entry Date(s): | Date Created: 20251001 Date Completed: 20251118 Latest Revision: 20251118 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.foodchem.2025.146509 |
| PMID: | 41033027 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-7072 |
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| DOI: | 10.1016/j.foodchem.2025.146509 |