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Cellular and Mechanical Responses to Zinc Polycarboxylate Cement Modified With 5% or 10% Potassium Nitrate: An In Vitro Study.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Cellular and Mechanical Responses to Zinc Polycarboxylate Cement Modified With 5% or 10% Potassium Nitrate: An In Vitro Study.
Συγγραφείς: Dahlawi F; Fellowship in Endodontics Program, Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., AlGharni M; Fellowship in Endodontics Program, Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Madi M; Department of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Ayad NM; Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Alonizan F; Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Aldossary N; Department of Pathology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Omar O; Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Πηγή: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc [Aust Endod J] 2026 Apr; Vol. 52 (1), pp. 59-69. Date of Electronic Publication: 2025 Sep 25.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley-Blackwell Publishing Asia Country of Publication: Australia NLM ID: 100888004 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1747-4477 (Electronic) Linking ISSN: 13291947 NLM ISO Abbreviation: Aust Endod J Subsets: MEDLINE
Imprint Name(s): Publication: Richmond, VIC : Wiley-Blackwell Publishing Asia
Original Publication: Melbourne, Vic. : Australian Society of Endodontology
Ιατρικοί όροι (MeSH): Nitrates*/pharmacology , Nitrates*/chemistry , Potassium Compounds*/pharmacology , Potassium Compounds*/chemistry , Leukocytes, Mononuclear*/drug effects , Polycarboxylate Cement*/pharmacology , Polycarboxylate Cement*/chemistry , Dental Cements*/pharmacology , Zinc Compounds*/pharmacology, Cell Survival/drug effects ; Silicates/pharmacology ; Calcium Compounds/pharmacology ; Oxides/pharmacology ; Aluminum Compounds/pharmacology ; Humans ; Materials Testing ; In Vitro Techniques ; Drug Combinations ; Compressive Strength ; Cells, Cultured ; Hydrogen-Ion Concentration
Περίληψη: This study investigated the biological response of human peripheral blood mononuclear cells (PBMCs) to zinc polycarboxylate (ZP) cement modified with 5% or 10% potassium nitrate (KNO3) and assessed its mechanical properties. Discs of unmodified ZP, ZP + 5% KNO3, ZP + 10% KNO3, or mineral trioxide aggregate (MTA) were indirectly exposed to PBMCs using a transwell system. Cells were incubated with or without lipopolysaccharide (LPS) stimulation. Cytotoxicity (LDH release), viability, IL-6 and TGF-β secretion were evaluated. Material pH, ion release, compressive strength and microhardness were also assessed. ZP + 5% KNO3 significantly reduced LDH and IL-6 levels and increased TGF-β release compared to ZP alone. Mechanical strength was maintained in ZP + 5% KNO3 but decreased at 10% KNO3. MTA showed lower strength but higher cell viability. ZP + 5% KNO3 demonstrated a balanced profile of immunomodulation, regenerative signalling and mechanical integrity, supporting its potential use in vital pulp therapy. Further in vivo investigations are recommended.
(© 2025 Australian Society of Endodontology Inc.)
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Grant Information: 12949-iau-2023-iau-R-3-1-HW Research, Development, and Innovation Authority (RDIA), Saudi Arabia, Riyadh, Reactivating & Rebuilding of Existing Labs Initiative
Contributed Indexing: Keywords: cytokines; cytotoxicity; potassium nitrate; vital pulp therapy; zinc polycarboxylate cement
Substance Nomenclature: 0 (Nitrates)
0 (Potassium Compounds)
RU45X2JN0Z (potassium nitrate)
0 (Polycarboxylate Cement)
0 (Silicates)
0 (mineral trioxide aggregate)
0 (Calcium Compounds)
0 (Drug Combinations)
0 (Dental Cements)
0 (Oxides)
0 (Zinc Compounds)
0 (Aluminum Compounds)
Entry Date(s): Date Created: 20250925 Date Completed: 20260406 Latest Revision: 20260406
Update Code: 20260406
DOI: 10.1111/aej.70022
PMID: 40995971
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1747-4477
DOI:10.1111/aej.70022