An electrochemiluminescence biosensor based on Ag-Ag2S as an efficient emitter and Zr-MOG as the co-reaction promoter for the ultrasensitive detection of CYFRA21-1.

Bibliographic Details
Title: An electrochemiluminescence biosensor based on Ag-Ag2S as an efficient emitter and Zr-MOG as the co-reaction promoter for the ultrasensitive detection of CYFRA21-1.
Authors: Wang Y; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Li F; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Liao X; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Liu K; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Hu L; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Ma H; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Wu D; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com., Wei Q; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China. hulihua1206@163.com.
Source: Analytical methods : advancing methods and applications [Anal Methods] 2025 Oct 02; Vol. 17 (38), pp. 7635-7642. Date of Electronic Publication: 2025 Oct 02.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Language: English
Journal Info: Publisher: RSC Pub Country of Publication: England NLM ID: 101519733 Publication Model: Electronic Cited Medium: Internet ISSN: 1759-9679 (Electronic) Linking ISSN: 17599660 NLM ISO Abbreviation: Anal Methods Subsets: MEDLINE
Imprint Name(s): Original Publication: Cambridge : RSC Pub., 2009-
MeSH Terms: Biosensing Techniques*/methods , Luminescent Measurements*/methods , Keratin-19*/analysis , Keratin-19*/blood , Antigens, Neoplasm*/analysis , Antigens, Neoplasm*/blood , Electrochemical Techniques*/methods , Silver Compounds*/chemistry, Metal Nanoparticles/chemistry ; Silver/chemistry ; Zirconium/chemistry ; Humans ; Limit of Detection
Abstract: In this work, a highly sensitive electrochemiluminescence (ECL) biosensor was successfully developed for the detection of CYFRA21-1, in which a metal-organic gel (Zr-MOG) was introduced as a novel co-reaction promoter for amplifying the ECL signal of the silver nanoparticle-modified silver sulfide/potassium persulfate (Ag-Ag2S/K2S2O8) system. Specifically, the incorporation of Ag nanoparticles narrowed the bandgap and enhanced the ECL signals of the composites. As a co-reaction promoter, Zr-MOG accelerated the reduction of S2O82- to produce abundant sulfate radicals (SO4˙-) while increasing the electrode active surface area, which led to a significant enhancement of the Ag-Ag2S ECL emission. The biosensor constructed based on the dual-signal amplification strategy demonstrated excellent sensitivity, selectivity, and stability, with a wide linear detection range of 0.00005-100 ng mL-1 and a detection limit as low as 17.66 fg mL-1 (S/N = 3).
Substance Nomenclature: 0 (antigen CYFRA21.1)
0 (Keratin-19)
0 (Antigens, Neoplasm)
0 (Silver Compounds)
9ZB10YHC1C (silver sulfide)
3M4G523W1G (Silver)
C6V6S92N3C (Zirconium)
Entry Date(s): Date Created: 20250918 Date Completed: 20251004 Latest Revision: 20260728
Update Code: 20260728
DOI: 10.1039/d5ay00896d
PMID: 40964957
Database: MEDLINE
Description
ISSN:1759-9679
DOI:10.1039/d5ay00896d