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Smartphone-based colorimetric detection of urease activity in dental plaque for point-of-care screening of Helicobacter pylori infection.

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Τίτλος: Smartphone-based colorimetric detection of urease activity in dental plaque for point-of-care screening of Helicobacter pylori infection.
Συγγραφείς: Wang Y; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.; Guangdong Zhongxin Biotech Limited, Guangzhou, 510000, China., Zhang P; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.; Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, 510632, China., Zhou C; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China., Zheng Z; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China., Quan Q; Research Center of Cancer Diagnosis and Therapy, Department of Oncology, the First Affiliated Hospital, Jinan University, Guangzhou, 510632, China., He G; Guangdong Zhongxin Biotech Limited, Guangzhou, 510000, China., Zhai H; Endoscopy Center, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, China., Song Q; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China., Zhang X; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.; Guangdong Zhongxin Biotech Limited, Guangzhou, 510000, China., Li H; Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou, 510000, China., Peng L; Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou, 510000, China., Liao Y; Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou, 510000, China., Fang W; Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou, 510000, China. fangwei@gdph.org.cn., Li M; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. limm269@126.com.; Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, 510632, China. limm269@126.com., Liang J; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China. 631101522@qq.com.; Research Center of Cancer Diagnosis and Therapy, Department of Oncology, the First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. 631101522@qq.com.; Guangdong Zhongxin Biotech Limited, Guangzhou, 510000, China. 631101522@qq.com., Tang Y; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China. tyjaq7926@163.com.; Guangdong Zhongxin Biotech Limited, Guangzhou, 510000, China. tyjaq7926@163.com.
Πηγή: Mikrochimica acta [Mikrochim Acta] 2025 Sep 13; Vol. 192 (10), pp. 666. Date of Electronic Publication: 2025 Sep 13.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE
Imprint Name(s): Original Publication: Wien ; New York : Springer-Verlag.
Ιατρικοί όροι (MeSH): Colorimetry*/methods , Colorimetry*/instrumentation , Urease*/metabolism , Urease*/analysis , Helicobacter pylori*/enzymology , Helicobacter pylori*/isolation & purification , Helicobacter Infections*/diagnosis , Helicobacter Infections*/microbiology , Dental Plaque*/microbiology , Dental Plaque*/enzymology , Smartphone* , Point-of-Care Testing*, Humans ; Limit of Detection
Περίληψη: Helicobacter pylori (H. pylori) is a major pathogen associated with gastric ulcers and gastric cancer, infecting approximately half of the global population. Therefore, developing sensitive, rapid, and cost-effective detection methods for H. pylori is critical for public health. We developed an innovative microscale colorimetric microsensor integrated with smartphone ambient-light sensors for portable, on-site quantification of H. pylori-associated urease activity in dental plaque. This low-cost device (< $ 5 fabrication) employs 3D-printed components and a custom app to measure transmittance changes from urease-catalyzed urea hydrolysis with phenol red, achieving a robust correlation with commercial microplate reader absorbance (R2 = 0.9989). The system demonstrates exceptional Analytical sensitivity, with limits of detection of 9.75 U/L for urease And 1.709 × 103 CFU/mL for H. pylori, alongside high reproducibility (CV < 10%) And stability over 14 days. When applied to the on-site detection of dental plaque among volunteers, the smartphone-based colorimetric microsensor exhibited detection results that were highly aligned with H. pylori infections confirmed by the urea breath test. It outperformed commercial test strips And achieved 100% sensitivity, 95.5% specificity, And 97.1% accuracy. This miniaturized, smartphone-based platform offers a promising solution for home self-testing and demonstrates significant potential for enhancing H. pylori preliminary screening at the community level.
(© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Competing Interests: Declarations. Ethics approval: Sample collection and processing followed WHO standards and were approved by the First Affiliated Hospital of Jinan University’s Ethics Committee (approval number: KY-2025–160). Patients or their legal representatives had provided informed consent. Competing interests: The authors declare no competing interests.
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Grant Information: A2023493 Medical Scientific Research Foundation of Guangdong Province; 2023CXB014 Outstanding Innovative Talents Cultivation Funded Programs for Doctoral Students of Jinan University; 2023B110008 Guangdong Provincial Clinical Research Center for Laboratory Medicine; 21625329 Fundamental Research Funds for the Central Universities; 32301266 National Natural Science Foundation of China; 2025B01J0005 Fundamental Research Funds for the Central Universities (21625329), Guangzhou Science and Technology Program Project; 2025-010C Young Talents Support Program - Seedling Cultivation Program (Class C) at the College of Life Science and Technology, Jinan University
Contributed Indexing: Keywords: Helicobacter pylori; Dental plaque; On-site detection; Smartphone-based colorimetric microsensor; Urease
Substance Nomenclature: EC 3.5.1.5 (Urease)
Entry Date(s): Date Created: 20250913 Date Completed: 20250916 Latest Revision: 20260810
Update Code: 20260811
DOI: 10.1007/s00604-025-07496-0
PMID: 40944762
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1436-5073
DOI:10.1007/s00604-025-07496-0