Academic Journal
Smartphone-assisted colorimetric detection of FEN1 and its inhibitors via RCA-magnetic beads-urease cascade amplification.
| Τίτλος: | Smartphone-assisted colorimetric detection of FEN1 and its inhibitors via RCA-magnetic beads-urease cascade amplification. |
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| Συγγραφείς: | Talap J; College of Pharmacy, Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi, 830017, China., Tang D; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, China., Wang Y; College of Pharmacy, Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi, 830017, China., Sun J; College of Pharmacy, Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi, 830017, China. Electronic address: sun@xjmu.edu.cn., Aisa HA; College of Pharmacy, Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi, 830017, China; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, China. Electronic address: haji@ms.xjb.ac.cn. |
| Πηγή: | Biosensors & bioelectronics [Biosens Bioelectron] 2025 Dec 15; Vol. 290, pp. 117969. Date of Electronic Publication: 2025 Sep 05. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| Ιατρικοί όροι (MeSH): | Flap Endonucleases*/antagonists & inhibitors , Flap Endonucleases*/isolation & purification , Flap Endonucleases*/analysis , Colorimetry*/methods , Colorimetry*/instrumentation , Biosensing Techniques*/methods , Biosensing Techniques*/instrumentation , Urease*/chemistry , Enzyme Inhibitors*/analysis , Smartphone* , Nucleic Acid Amplification Techniques*, Humans ; Limit of Detection |
| Περίληψη: | Given the pivotal role of Flap endonuclease 1 (FEN1) in tumor pathogenesis and progression, the advancement of its activity and inhibitor assays holds significant importance for cancer research and drug screening. Herein, we proposed a convenient, visual and sensitive colorimetric biosensing platform for FEN1 activity detection by integrating the robust signal amplification power of rolling circle amplification (RCA), the target enrichment capability of magnetic beads (MB), and the high efficiency and visualization of urease-mediated litmus test. Based on the significant color transition with a clear response mechanism, quantitative analysis can be achieved by either spectroscopic or smartphone-based detection. The smartphone-assisted approach demonstrated excellent FEN1 activity detection performance within the range of 2 × 10-6 U/μL to 0.02 U/μL, with a detection limit of 1.5 × 10-6 U/μL. The practical utility of this method was validated by assessing differential FEN1 expression profiles in both cytoplasmic and nuclear protein extracts of lung cancer cells versus bronchial epithelial cells. Furthermore, experimental verification confirmed that our urease-mediated system demonstrates enhanced signal-to-noise ratio due to its insusceptibility to the peroxidase-like activity of Fe (Copyright © 2025 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Cascade signal amplification; Flap endonuclease 1; Inhibitors detection; Rolling circle amplification (RCA); Urease catalysis |
| Substance Nomenclature: | EC 3.1.- (Flap Endonucleases) EC 3.1.11.- (FEN1 protein, human) EC 3.5.1.5 (Urease) 0 (Enzyme Inhibitors) |
| Entry Date(s): | Date Created: 20250910 Date Completed: 20251003 Latest Revision: 20251003 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.bios.2025.117969 |
| PMID: | 40929784 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2025.117969 |