Brief Electrical Stimulation of the Distal and Proximal Sciatic Nerve During Transection and Repair in a Mouse Model.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Brief Electrical Stimulation of the Distal and Proximal Sciatic Nerve During Transection and Repair in a Mouse Model.
Συγγραφείς:	Puder JM; Division of Plastic and Reconstructive Surgery, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA., Jablonka-Shariff A; Division of Plastic and Reconstructive Surgery, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA., Wood MD; Division of Plastic and Reconstructive Surgery, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA., Snyder-Warwick AK; Division of Plastic and Reconstructive Surgery, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA.
Πηγή:	Muscle & nerve [Muscle Nerve] 2025 Nov; Vol. 72 (5), pp. 1178-1183. Date of Electronic Publication: 2025 Sep 10.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 7803146 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4598 (Electronic) Linking ISSN: 0148639X NLM ISO Abbreviation: Muscle Nerve Subsets: MEDLINE
Imprint Name(s):	Publication: <2005-> : Hoboken, NJ : John Wiley & Sons Original Publication: New York, NY : John Wiley & Sons
Ιατρικοί όροι (MeSH):	Sciatic Nerve/injuries , Sciatic Nerve/physiology , Electric Stimulation Therapy/methods , Nerve Regeneration/physiology, Neuromuscular Junction/physiology ; Recovery of Function/physiology ; Muscle, Skeletal/innervation ; Muscle, Skeletal/physiopathology ; Electric Stimulation/methods ; S100 Proteins/genetics ; S100 Proteins/metabolism ; Animals ; Mice ; Mice, Transgenic ; Disease Models, Animal ; Mice, Inbred C57BL ; Male
Περίληψη:	Introduction/aims: Therapeutic electrical stimulation (ES) of repaired nerves has been demonstrated to improve muscle function. Previous studies applied ES to the proximal transected nerve end (P-ES) with benefits to the neuronal cell body. We investigated whether a single ES dose applied to the distal end (D-ES) or distal and proximal ends (DP-ES) prior to nerve repair provides benefits to neuromuscular junction (NMJ) and muscle recovery. Methods: S100-GFP mice were randomized to control (No ES), D-ES, P-ES, and DP-ES groups. After sciatic nerve transection, the ES groups received 10 min of ES prior to repair. The non-stimulated nerve end was insulated with a rubberized wrap. At 3 and 4 weeks post-repair, analyses using tetanic muscle force, grid walking, and NMJ immunohistochemistry were performed. Results: Muscle force demonstrated D-ES, P-ES, and DP-ES significantly improved muscle function at 4 weeks compared to No ES. Grid-walking tests showed significantly fewer foot faults in D-ES and DP-ES mice. Mice treated with ES showed higher NMJ innervation compared to No ES. In addition, the number of terminal Schwann cells (tSCs) and the percentage of NMJs with tSC sprouting beyond the NMJ area were significantly higher in ES groups, indicating improved innervation. Endplate morphology was similar among ES groups. Discussion: In addition to known benefits of P-ES, D-ES and P-ES demonstrated beneficial effects for reinnervation and muscle function post-nerve injury. While the mechanism is not fully characterized, ES applied to both D-ES and P-ES aspects of a transected nerve may act synergistically to improve muscle healing. (© 2025 Wiley Periodicals LLC.)
References:	A. Althagafi and M. Nadi, “Acute Nerve Injury,” in StatPearls (StatPearls Publishing LLC, 2024). A. A. Al‐Majed, C. M. Neumann, T. M. Brushart, and T. Gordon, “Brief Electrical Stimulation Promotes the Speed and Accuracy of Motor Axonal Regeneration,” Journal of Neuroscience 20, no. 7 (2000): 2602–2608, https://doi.org/10.1523/JNEUROSCI.20‐07‐02602.2000. K. J. Zuo, T. Gordon, K. M. Chan, and G. H. Borschel, “Electrical Stimulation to Enhance Peripheral Nerve Regeneration: Update in Molecular Investigations and Clinical Translation,” Experimental Neurology 332 (2020): 113397, https://doi.org/10.1016/j.expneurol.2020.113397. S. P. Frostick, Q. Yin, and G. J. Kemp, “Schwann Cells, Neurotrophic Factors, and Peripheral Nerve Regeneration,” Microsurgery 18, no. 7 (1998): 397–405. N. A. McLean and V. M. Verge, “Dynamic Impact of Brief Electrical Nerve Stimulation on the Neural Immune Axis‐Polarization of Macrophages Toward a Pro‐Repair Phenotype in Demyelinated Peripheral Nerve,” Glia 64, no. 9 (2016): 1546–1561, https://doi.org/10.1002/glia.23021. S. C. Ransom, S. Shahrestani, B. V. Lien, et al., “Translational Approaches to Electrical Stimulation for Peripheral Nerve Regeneration,” Neurorehabilitation and Neural Repair 34, no. 11 (2020): 979–985, https://doi.org/10.1177/1545968320962508. J. Sayanagi, J. A. Acevedo‐Cintrón, D. Pan, et al., “Brief Electrical Stimulation Accelerates Axon Regeneration and Promotes Recovery Following Nerve Transection and Repair in Mice,” Journal of Bone and Joint Surgery. American Volume 103, no. 20 (2021): e80, https://doi.org/10.2106/JBJS.20.01965. B. Barber, M. McNeely, K. M. Chan, et al., “Intraoperative Brief Electrical Stimulation (BES) for Prevention of Shoulder Dysfunction After Oncologic Neck Dissection: Study Protocol for a Randomized Controlled Trial,” Trials 16 (2015): 240, https://doi.org/10.1186/s13063‐015‐0745‐7. T. Gordon, N. Amirjani, D. C. Edwards, and K. M. Chan, “Brief Post‐Surgical Electrical Stimulation Accelerates Axon Regeneration and Muscle Reinnervation Without Affecting the Functional Measures in Carpal Tunnel Syndrome Patients,” Experimental Neurology 223, no. 1 (2010): 192–202, https://doi.org/10.1016/j.expneurol.2009.09.020. H. A. Power, M. J. Morhart, J. L. Olson, and K. M. Chan, “Postsurgical Electrical Stimulation Enhances Recovery Following Surgery for Severe Cubital Tunnel Syndrome: A Double‐Blind Randomized Controlled Trial,” Neurosurgery 86, no. 6 (2020): 769–777, https://doi.org/10.1093/neuros/nyz322. J. N. Wong, J. L. Olson, M. J. Morhart, and K. M. Chan, “Electrical Stimulation Enhances Sensory Recovery: A Randomized Controlled Trial,” Annals of Neurology 77, no. 6 (2015): 996–1006, https://doi.org/10.1002/ana.24397. C. Calvey, W. Zhou, K. S. Stakleff, et al., “Short‐Term Electrical Stimulation to Promote Nerve Repair and Functional Recovery in a Rat Model,” Journal of Hand Surgery 40, no. 2 (2015): 314–322, https://doi.org/10.1016/j.jhsa.2014.10.002. X. Li, T. Zhang, C. Li, et al., “Electrical Stimulation Accelerates Wallerian Degeneration and Promotes Nerve Regeneration After Sciatic Nerve Injury,” Glia 71, no. 3 (2023): 758–774, https://doi.org/10.1002/glia.24309. A. Jablonka‐Shariff, E. Balta, K. B. Santosa, C. Y. Lu, and A. K. Snyder‐Warwick, “Terminal Schwann Cells Are Essential for Neuromuscular Junction Function and Recovery After Nerve Injury,” Plastic and Reconstructive Surgery 151, no. 4 (2023): 792–803, https://doi.org/10.1097/PRS.0000000000009999. G. Feng, R. H. Mellor, M. Bernstein, et al., “Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP,” Neuron 28, no. 1 (2000): 41–51, https://doi.org/10.1016/s0896‐6273(00)00084‐2. E. B. Marsh, L. Schellhardt, D. A. Hunter, S. E. Mackinnon, A. K. Snyder‐Warwick, and M. D. Wood, “Electrical Stimulation or Tacrolimus (FK506) Alone Enhances Nerve Regeneration and Recovery After Nerve Surgery, While Dual Use Reduces Variance and Combines Strengths of Each in Promoting Enhanced Outcomes,” Muscle & Nerve 67, no. 1 (2023): 78–87, https://doi.org/10.1002/mus.27748. S. Jo, D. Pan, A. E. Halevi, et al., “Comparing Electrical Stimulation and Tacrolimus (FK506) to Enhance Treating Nerve Injuries,” Muscle & Nerve 60, no. 5 (2019): 629–636, https://doi.org/10.1002/mus.26659. D. Pan, D. A. Hunter, L. Schellhardt, et al., “T Cells Modulate IL‐4 Expression by Eosinophil Recruitment Within Decellularized Scaffolds to Repair Nerve Defects,” Acta Biomaterialia 112 (2020): 149–163, https://doi.org/10.1016/j.actbio.2020.05.009. K. B. Santosa, N. J. Jesuraj, A. Viader, et al., “Nerve Allografts Supplemented With Schwann Cells Overexpressing Glial‐Cell‐Line‐Derived Neurotrophic Factor,” Muscle & Nerve 47, no. 2 (2013): 213–223, https://doi.org/10.1002/mus.23490. A. Jablonka‐Shariff, C. Y. Lu, K. Campbell, K. R. Monk, and A. K. Snyder‐Warwick, “Gpr126/Adgrg6 Contributes to the Terminal Schwann Cell Response at the Neuromuscular Junction Following Peripheral Nerve Injury,” Glia 68, no. 6 (2020): 1182–1200, https://doi.org/10.1002/glia.23769. A. K. Snyder‐Warwick, A. Satoh, K. B. Santosa, S. I. Imai, and A. Jablonka‐Shariff, “Hypothalamic Sirt1 Protects Terminal Schwann Cells and Neuromuscular Junctions From Age‐Related Morphological Changes,” Aging Cell 17, no. 4 (2018): e12776, https://doi.org/10.1111/acel.12776. M. P. Willand, E. Rosa, B. Michalski, et al., “Electrical Muscle Stimulation Elevates Intramuscular BDNF and GDNF mRNA Following Peripheral Nerve Injury and Repair in Rats,” Neuroscience 334 (2016): 93–104, https://doi.org/10.1016/j.neuroscience.2016.07.040. L. Juckett, T. M. Saffari, B. Ormseth, J. L. Senger, and A. M. Moore, “The Effect of Electrical Stimulation on Nerve Regeneration Following Peripheral Nerve Injury,” Biomolecules 12, no. 12 (2022): 1856, https://doi.org/10.3390/biom12121856. M. P. Willand, M. A. Nguyen, G. H. Borschel, and T. Gordon, “Electrical Stimulation to Promote Peripheral Nerve Regeneration,” Neurorehabilitation and Neural Repair 30, no. 5 (2016): 490–496, https://doi.org/10.1177/1545968315604399. Y. C. Lin, C. H. Kao, Y. K. Cheng, J. J. Chen, C. H. Yao, and Y. S. Chen, “Current‐Modulated Electrical Stimulation as a Treatment for Peripheral Nerve Regeneration in Diabetic Rats,” Restorative Neurology and Neuroscience 32, no. 3 (2014): 437–446, https://doi.org/10.3233/RNN‐130381. C. J. Chang and S. H. Hsu, “The Effects of Low‐Intensity Ultrasound on Peripheral Nerve Regeneration in Poly(DL‐Lactic Acid‐Co‐Glycolic Acid) Conduits Seeded With Schwann Cells,” Ultrasound in Medicine & Biology 30, no. 8 (2004): 1079–1084, https://doi.org/10.1016/j.ultrasmedbio.2004.06.005. A. Ito, Y. Araya, H. Kawai, and H. Kuroki, “Ultrasound Stimulation Inhibits Morphological Degeneration of Motor Endplates in the Denervated Skeletal Muscle of Rats,” Neuroscience Insights 17 (2022): 26331055221138508, https://doi.org/10.1177/26331055221138508. C. Ren, X. Chen, N. Du, et al., “Low‐Intensity Pulsed Ultrasound Promotes Schwann Cell Viability and Proliferation via the GSK‐3β/β‐Catenin Signaling Pathway,” International Journal of Biological Sciences 14, no. 5 (2018): 497–507, https://doi.org/10.7150/ijbs.22409. Y. H. Tsuang, L. W. Liao, Y. H. Chao, et al., “Effects of Low Intensity Pulsed Ultrasound on Rat Schwann Cells Metabolism,” Artificial Organs 35, no. 4 (2011): 373–383, https://doi.org/10.1111/j.1525‐1594.2010.01086.x. A. R. R. H. Hamid, S. Maliawan, D. P. G. P. Samatra, et al., “Effect of Immediate Electrical Stimulation in the Distal Segment of the Nerve With Wallerian Degeneration in Rats With Sciatic Nerve Injury,” Medical Journal of Indonesia 31, no. 1 (2022): 7–12, https://doi.org/10.13181/mji.oa.225870.
Grant Information:	Checkpoint Surgical; Plastic Surgery Foundation; United States NH NIH HHS
Contributed Indexing:	Keywords: electrical stimulation; endplate reinnervation; nerve injury; neuromuscular junction; terminal Schwann cell sprouting
Substance Nomenclature:	0 (S100 Proteins)
Entry Date(s):	Date Created: 20250910 Date Completed: 20251016 Latest Revision: 20251016
Update Code:	20260130
DOI:	10.1002/mus.70020
PMID:	40926617
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1097-4598
DOI:	10.1002/mus.70020