Performance and stability of urease inhibitors: Complementary effects of phenyl phosphorodiamidate and N-(n-butyl)thiophosphoric triamide under pH and temperature variations.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Performance and stability of urease inhibitors: Complementary effects of phenyl phosphorodiamidate and N-(n-butyl)thiophosphoric triamide under pH and temperature variations.
Συγγραφείς:	Jeon YJ; Department of Environmental Engineering, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju 28644, Republic of Korea., Ahn JH; Department of Environmental Engineering, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju 28644, Republic of Korea., Yun YM; Department of Environmental Engineering, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju 28644, Republic of Korea. Electronic address: ymyun@chungbuk.ac.kr.
Πηγή:	Bioresource technology [Bioresour Technol] 2025 Dec; Vol. 438, pp. 133267. Date of Electronic Publication: 2025 Sep 03.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s):	Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
Ιατρικοί όροι (MeSH):	Urease/antagonists & inhibitors , Urease/metabolism , Enzyme Inhibitors/pharmacology , Enzyme Inhibitors/chemistry , Organothiophosphorus Compounds/pharmacology , Temperature, Enzyme Stability/drug effects ; Hydrogen-Ion Concentration ; Molecular Docking Simulation ; Animals ; Ammonia ; Urea ; Manure ; Organophosphorus Compounds
Περίληψη:	Ammonia volatilization from livestock manure is driven by urease-catalyzed urea hydrolysis, strongly influenced by temperature and pH. This study assessed the inhibition performance of phenyl phosphorodiamidate (PPDA) and N-(n-butyl)thiophosphoric triamide (NBPT) under conditions (10-35°C, pH 6-10) representative of manure storage. PPDA achieved strong suppression at 10°C and pH 6, reducing ammonia below 7.0 g NH4⁺-N/L (over 75 % reduction compared to control), but declined under elevated conditions. NBPT was less effective than PPDA under mild conditions but maintained stable performance as temperature and pH increased. Their combined use delayed ammonia peaks by up to 10 days, lowered concentrations by 6-8 %, and yielded the lowest reaction rate (k = 0.03 d^-1) and highest activation energy (Ea = 59.4 ± 2.8 kJ/mol). Molecular docking confirmed PPDA formed multiple but weaker bonds, while NBPT provided shorter and stable interactions. Dual inhibition strengthens urease suppression, offering practical insights for seasonal and storage-specific application. (Copyright © 2025. Published by Elsevier Ltd.)
Competing Interests:	Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing:	Keywords: Ammonia mitigation; Kinetic modeling; Molecular docking; Swine manure storage; Urea; Urease inhibition
Substance Nomenclature:	EC 3.5.1.5 (Urease) 0 (N-(n-butyl) thiophosphoric triamide) 0 (Enzyme Inhibitors) 7664-41-7 (Ammonia) 0 (Organothiophosphorus Compounds) 8W8T17847W (Urea) 0 (Manure) 0 (Organophosphorus Compounds)
Entry Date(s):	Date Created: 20250905 Date Completed: 20250916 Latest Revision: 20260127
Update Code:	20260130
DOI:	10.1016/j.biortech.2025.133267
PMID:	40912575
Βάση Δεδομένων:	MEDLINE

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Περιγραφή
ISSN:	1873-2976
DOI:	10.1016/j.biortech.2025.133267