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Genomic Analysis and Biomineralization Efficacy of Bacillus megaterium SS3 for Improving Durability Properties of Building Material.

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Τίτλος: Genomic Analysis and Biomineralization Efficacy of Bacillus megaterium SS3 for Improving Durability Properties of Building Material.
Συγγραφείς: Sharma B; Department of Biotechnology, Thapar Institute of Engineering & Technology, Patiala, 147004, Punjab, India., Sharma S; Department of Civil Engineering, Thapar Institute of Engineering & Technology, Patiala, 147004, Punjab, India., Medicherla KM; School of Life and Basic Sciences, Jaipur National University, Jagatpura, Jaipur, 302017, India., Reddy SM; Department of Biotechnology, Thapar Institute of Engineering & Technology, Patiala, 147004, Punjab, India. msreddy@thapar.edu.
Πηγή: Molecular biotechnology [Mol Biotechnol] 2026 Apr; Vol. 68 (4), pp. 2037-2052. Date of Electronic Publication: 2025 Aug 07.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE
Imprint Name(s): Publication: [Cham] : Springer
Original Publication: Totowa, NJ : Humana Press, c1994-
Ιατρικοί όροι (MeSH): Bacillus megaterium*/genetics , Bacillus megaterium*/metabolism , Bacillus megaterium*/enzymology , Urease*/genetics , Urease*/metabolism , Urease*/chemistry , Construction Materials*/microbiology , Biomineralization*, Calcium Carbonate/metabolism ; Calcium Carbonate/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Proteins/chemistry ; Genomics/methods ; Urea/metabolism ; Molecular Docking Simulation ; Soil Microbiology ; Genome, Bacterial ; Whole Genome Sequencing
Περίληψη: Urease-producing microorganisms play an important role in biomineralization through microbially induced calcium carbonate precipitation (MICCP), contributing to enhanced durability and extended lifespan of construction materials in civil engineering. This study investigates the MICCP capabilities of a ureolytic strain, Bacillus megaterium SS3, isolated from alkaline calcareous soil, which showed native adaptation to high-pH environments typical of cementitious materials. Bacillus megaterium exhibited maximum urease activity (625 U/mL) and promoted CaCO3 precipitation up to 177.3 mg/100 mL. Its incorporation into cement mortar enhanced compressive strength by 18.9% and 10.58% at 7 and 28 days of curing, respectively, and significantly reduced water absorption compared to control specimens. Whole-genome sequencing and gene annotation revealed three structural urease genes (ureA, ureB, ureC) and four accessory urease genes (ureD, ureE, ureF, ureG), providing molecular insight into its biomineralization potential. To validate structure-function relationships, urease enzyme was modelled and molecular docking was performed with urea. The predicted structure showed strong binding at the catalytic site with key residues and nickel ions, confirming enzymatic suitability for MICCP. Bacillus megaterium SS3 not only exhibits effective MICCP performance but also showed enhanced environmental resilience when incorporated into mortar structures, positioning it as a strong candidate for microbial biocementation in civil engineering applications. This is the first report to integrate genome annotation, protein docking, and real-world application in mortar, positioning B. megaterium SS3 as a novel, genome-validated, biomineralizing strain for sustainable construction.
(© 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors have no competing interests to declare relevant to this article’s content. Informed Consent: Not applicable. Research Involving Human Participants and/or Animals: Not applicable.
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Grant Information: CRG/2020/005275 Science and Engineering Research Board, Ministry of Science & Technology, Government of India
Contributed Indexing: Keywords: Biomineralization; Cement mortar; Compressive strength; MICCP; Urease; Water absorption
Substance Nomenclature: EC 3.5.1.5 (Urease)
H0G9379FGK (Calcium Carbonate)
0 (Bacterial Proteins)
8W8T17847W (Urea)
Entry Date(s): Date Created: 20250807 Date Completed: 20260406 Latest Revision: 20260406
Update Code: 20260406
DOI: 10.1007/s12033-025-01491-9
PMID: 40773149
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1559-0305
DOI:10.1007/s12033-025-01491-9