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Overexpression of Agr quorum sensing system in uropathogenic Staphylococcus aureus in response to simulated urinary metabolic conditions enhancing growth and virulence.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Overexpression of Agr quorum sensing system in uropathogenic Staphylococcus aureus in response to simulated urinary metabolic conditions enhancing growth and virulence.
Συγγραφείς: Subramaniyan Y; Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Mangalore, 575018, India., Mujeeburahiman M; Department of Urology, Yenepoya Medical College and Hospital, Yenepoya (Deemed to be University), University Road, Mangalore, 575018, India., Khan A; Department of Urology, Yenepoya Medical College and Hospital, Yenepoya (Deemed to be University), University Road, Mangalore, 575018, India., Rekha PD; Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Mangalore, 575018, India. rekhapd@yenepoya.edu.in.
Πηγή: Medical microbiology and immunology [Med Microbiol Immunol] 2025 Aug 05; Vol. 214 (1), pp. 35. Date of Electronic Publication: 2025 Aug 05.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0314524 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-1831 (Electronic) Linking ISSN: 03008584 NLM ISO Abbreviation: Med Microbiol Immunol Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, New York, Springer-Verlag.
Ιατρικοί όροι (MeSH): Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism , Bacterial Proteins*/biosynthesis , Staphylococcus aureus*/pathogenicity , Staphylococcus aureus*/growth & development , Staphylococcus aureus*/genetics , Staphylococcus aureus*/physiology , Urinary Tract Infections*/microbiology , Trans-Activators*/genetics , Trans-Activators*/metabolism , Trans-Activators*/biosynthesis , Urine*/chemistry , Urine*/microbiology , Quorum Sensing*, Urease/genetics ; Urease/metabolism ; Biofilms/growth & development ; Staphylococcal Infections/microbiology ; Virulence Factors/genetics ; Virulence ; Humans ; Gene Expression Regulation, Bacterial ; Hydrogen-Ion Concentration
Περίληψη: Staphylococcus aureus (S. aureus) is a major opportunistic pathogen, causing acute and chronic infections including urinary tract infection (UTI). S. aureus relies on the Accessory gene regulator (Agr), a central quorum sensing (QS) system and the urease genes ureABCEFGD, for the regulation of urease expression and its pathogenicity. Urease is a key virulence factor for S. aureus, modulating immune responses by altering the local urinary pH and impairing immune defenses in the hostile urinary environment. QS is regulated by the external stimuli and in urological disease, the role of different urinary metabolite conditions and pH on the urease expression and Agr-QS regulation remains poorly understood. In this study, we explored the growth, biofilm formation, and urease activity under various simulated urinary metabolic and pH environments to study the response of S. aureus strains isolated from patients with urological diseases. The expression levels of QS genes and urease genes were compared in different urinary conditions that included the conditions from which the strains were isolated. A correlation analysis was used to study the associations between growth, pH changes, urease activity, biofilm formation, and the expression of agr and ure genes to predict their regulatory relationships. Our results demonstrated significant differences across glycosuria, haematuria, creatininuria, and albuminuria in growth, biofilm formation, and urease activity in S. aureus strains (p < 0.001). Significantly higher growth and urease activity were noted in glycosuria and haematuria-originated strains under the similar simulated conditions (p < 0.001). However, all the simulated conditions increased the expression levels of agr and ure genes; in the pre-adapted environment, favoring their survival highlighting niche adaptation. The simulated conditions with acidic pH significantly overexpressed the agr and ure genes compared to alkaline pH (p < 0.001). Increased expression profile of the QS system under the disease specific urine metabolic conditions, suggests its role in promoting bacterial fitness in the urinary environment and also forms the basis of managing UTI with targeted approach.
(© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Ethical approval: All the procedures in this study involving human participants were approved by the institutional ethics committee (Protocol no. YEC-1/2021/065), Yenepoya (Deemed to be University). Prior informed consent was obtained from the participants before the collection of urine samples. Consent to publish: All the participants have consented to use the data in the scientific publication without participant identifiers. Competing interests: The authors declare no competing interests.
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Substance Nomenclature: 0 (Bacterial Proteins)
EC 3.5.1.5 (Urease)
0 (Agr protein, Staphylococcus aureus)
0 (Trans-Activators)
0 (Virulence Factors)
Entry Date(s): Date Created: 20250805 Date Completed: 20250805 Latest Revision: 20251230
Update Code: 20260130
DOI: 10.1007/s00430-025-00830-6
PMID: 40762857
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1432-1831
DOI:10.1007/s00430-025-00830-6