Academic Journal
Effectiveness of filling structures within nerve guidance conduits in a rat sciatic nerve injury model.
| Τίτλος: | Effectiveness of filling structures within nerve guidance conduits in a rat sciatic nerve injury model. |
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| Συγγραφείς: | Kasuya T; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Nishimoto S; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Iwahashi T; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Sayanagi J; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Hirai Y; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Shimada T; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Yoshimura Y; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Konishi K; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Konishi M; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Shiode R; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Miyamura S; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Oka K; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Murase T; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Okada S; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan., Tanaka H; Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.; Department of Sports Medical Science, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. |
| Πηγή: | Regenerative therapy [Regen Ther] 2025 Jul 29; Vol. 30, pp. 446-455. Date of Electronic Publication: 2025 Jul 29 (Print Publication: 2025). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: The Japanese Society for Regenerative Medicine. Production and Hosting by Elsevier B.V Country of Publication: Netherlands NLM ID: 101709085 Publication Model: eCollection Cited Medium: Internet ISSN: 2352-3204 (Electronic) Linking ISSN: 23523204 NLM ISO Abbreviation: Regen Ther Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: [Amsterdam] : The Japanese Society for Regenerative Medicine. Production and Hosting by Elsevier B.V., [2015]- |
| Περίληψη: | Introduction: The gold standard treatment for peripheral nerve gap injury is nerve autograft transplantation. Although various nerve guidance conduits (NGCs) have been developed as alternatives to autografting, few reports have evaluated the effects of the internal structure of NGCs on nerve regeneration. We investigated how the internal structure of NGCs affects nerve regeneration. Methods: In 30 male Wistar rats, a 5 mm segment of the left sciatic nerve was resected, creating a gap. The animals were then randomly divided into two groups. A 7 mm polyglycolic acid (PGA) conduit, with (PGA-c group) or without a collagen filling (PGA group), was used to bridge the gap (n = 15 for each group). At 2 and 4 weeks postoperatively, longitudinal sciatic nerve slices were fluorescently immunostained with RECA-1 for endothelial cells, S100 for Schwann cells, and TUJ1 for axons. The fluorescence-positive areas were quantitatively evaluated. Next, 32 male Wistar rats underwent resection of a 10 mm segment of the left sciatic nerve. The animals were then assigned into four groups: sham group, autograft group, PGA-c group (transplantation of 12-mm PGA-c), and hollow PGA group (transplantation of 12 mm hollow PGA) (n = 8 for each group). At 12 weeks postoperatively, morphological evaluations and neurofunctional analyses were performed. Results: In longitudinal sciatic nerve slices, the PGA-c group had significantly larger RECA-1-positive areas proximally and distally at 2 weeks, larger S100-positive areas proximally at 2 weeks, and larger TUJ1-positive areas proximally at 4 weeks postoperatively than the PGA group. In the 10 mm nerve defect model, the PGA-c group had a significantly higher percentage of myelinated axons, isometric tetanic force, and tibialis anterior muscle wet weight than the PGA group. Conclusions: The internal filling structure of the NGCs may promote nerve regeneration by providing a scaffold for cells involved in nerve regeneration and may restore motor function. These findings provide new insights into the further structural development of NGCs suitable for peripheral nerve regeneration. (© 2025 The Author(s).) |
| Competing Interests: | The funding sponsor, 10.13039/100019953Toyobo Co., Ltd., had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. The authors have declared that no further conflict of interest exists. |
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| Contributed Indexing: | Keywords: Internal structure; Nerve guidance conduit; Peripheral nerve gap injury; Peripheral nerve regeneration; Polyglycolic acid |
| Entry Date(s): | Date Created: 20250805 Latest Revision: 20250807 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12320549 |
| DOI: | 10.1016/j.reth.2025.07.011 |
| PMID: | 40761372 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2352-3204 |
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| DOI: | 10.1016/j.reth.2025.07.011 |