Academic Journal
Spin Sensor-Integrated Covalent Organic Frameworks Reveal the Effect of Host-Guest Interactions on the Catalytic Activity of Immobilized Enzymes.
| Τίτλος: | Spin Sensor-Integrated Covalent Organic Frameworks Reveal the Effect of Host-Guest Interactions on the Catalytic Activity of Immobilized Enzymes. |
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| Συγγραφείς: | Feng J; School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China., Liu X; School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China., Huang S; Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China., Zhong H; School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China., Xue C; School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China., Chen G; MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China., Zhou X; School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China.; Huizhou Research Institute, Sun Yat-sen University, Huizhou 516081, China., Ouyang G; School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China.; MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China. |
| Πηγή: | Journal of the American Chemical Society [J Am Chem Soc] 2025 Aug 13; Vol. 147 (32), pp. 28912-28922. Date of Electronic Publication: 2025 Jul 30. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 7503056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5126 (Electronic) Linking ISSN: 00027863 NLM ISO Abbreviation: J Am Chem Soc Subsets: MEDLINE |
| Imprint Name(s): | Publication: Washington, DC : American Chemical Society Original Publication: Easton, Pa. [etc.] |
| Ιατρικοί όροι (MeSH): | Enzymes, Immobilized*/chemistry , Enzymes, Immobilized*/metabolism , Metal-Organic Frameworks*/chemistry , Metal-Organic Frameworks*/metabolism , Spin Labels*, Electron Spin Resonance Spectroscopy ; Biocatalysis |
| Περίληψη: | Encapsulating enzymes within covalent organic frameworks (COFs) offers a promising strategy to enhance enzyme stability and facilitate their separation and recycling. However, the complex host-guest interactions in such hybrid systems substantially affect enzyme conformation flexibility, which can dominate key catalytic processes such as substrate binding and catalytic conversion. Understanding these molecular interplays is crucial but remains challenging. In this work, we present a spin sensor-integrated COF (SSICOF) strategy to probe host-guest interactions across diverse enzyme@COF systems. This approach leverages the highly programmable reticular framework of structurally stable COFs, wherein pore channels are atomically engineered to integrate spin probes via a click chemistry reaction. The spin-lattice interactions between the spin probes and the encapsulated enzyme generate interpretable variations in electron paramagnetic resonance (EPR) signals, delivering the host-guest interactions that cannot be probed by traditional spectroscopic techniques. Using the SSICOF approach combined with computational simulations, we demonstrate that the catalytic activity of immobilized enzymes is closely linked to their degree of freedom and structural flexibility under COF pore confinement. This strategy offers a versatile and powerful tool for probing underlying interactions in complex host-guest systems and predicting the catalytic activity of enzyme@COF hybrid biocatalysts. |
| Substance Nomenclature: | 0 (Enzymes, Immobilized) 0 (Metal-Organic Frameworks) 0 (Spin Labels) |
| Entry Date(s): | Date Created: 20250730 Date Completed: 20250825 Latest Revision: 20260824 |
| Update Code: | 20260824 |
| DOI: | 10.1021/jacs.5c06528 |
| PMID: | 40736026 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1520-5126 |
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| DOI: | 10.1021/jacs.5c06528 |