Academic Journal
Sumner's legacy: a century of urease crystals and recent structural advances.
| Τίτλος: | Sumner's legacy: a century of urease crystals and recent structural advances. |
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| Συγγραφείς: | Hausinger RP; Department of Microbiology, Genetics, and Immunology and the Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA. |
| Πηγή: | Critical reviews in biochemistry and molecular biology [Crit Rev Biochem Mol Biol] 2025 Feb-Jun; Vol. 60 (1-3), pp. 123-140. Date of Electronic Publication: 2025 Jul 17. |
| Τύπος έκδοσης: | Journal Article; Historical Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 8903774 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-7798 (Electronic) Linking ISSN: 10409238 NLM ISO Abbreviation: Crit Rev Biochem Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : Informa Healthcare Original Publication: Boca Raton, Fla. : CRC Press, c1989- |
| Ιατρικοί όροι (MeSH): | Urease*/chemistry , Urease*/metabolism , Urease*/history, Nickel/chemistry ; Nickel/metabolism ; History, 20th Century ; History, 21st Century ; Humans ; Catalytic Domain ; Crystallization ; Crystallography, X-Ray |
| Περίληψη: | In 1926, James B. Sumner crystallized jack bean urease-the first enzyme to be obtained in crystalline form-thus demonstrating that enzymes are proteinaceous. To honor the 100-year anniversary of that momentous event, this review highlights critical findings leading up to Sumner's efforts, explains the significance of his results, and describes subsequent experimental findings related to urease. For example, nearly five decades after crystals became available Burt Zerner and colleagues identified urease as the first known nickel-containing enzyme. The surprising discovery of nickel in urease raised questions about the structure of the metal-containing active site, the enzyme mechanism, and pathway by which the catalytic center is synthesized - each of which is addressed here. Finally, I reflect on remaining open questions related to this remarkable enzyme and potential experimental directions that could be employed to provide corresponding insights. |
| Grant Information: | R01 DK045686 United States DK NIDDK NIH HHS |
| Contributed Indexing: | Keywords: Urease; crystal structure; enzyme mechanism; metallocenter biosynthesis; nickel |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 7OV03QG267 (Nickel) |
| Entry Date(s): | Date Created: 20250717 Date Completed: 20250806 Latest Revision: 20260227 |
| Update Code: | 20260227 |
| PubMed Central ID: | PMC12936839 |
| DOI: | 10.1080/10409238.2025.2533765 |
| PMID: | 40673339 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1549-7798 |
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| DOI: | 10.1080/10409238.2025.2533765 |