StreamFind: data processing workflows for qualification and quantification of biopharmaceutical drug products analyzed by size exclusion chromatography coupled to capillary-enhanced Raman spectroscopy.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	StreamFind: data processing workflows for qualification and quantification of biopharmaceutical drug products analyzed by size exclusion chromatography coupled to capillary-enhanced Raman spectroscopy.
Συγγραφείς:	Thissen J; Institut Für Umwelt & Energie, Technik & Analytik E.V. (IUTA), Bliersheimer Str. 58-60, 47229, Duisburg, Germany.; Faculty of Mathematics and Natural Sciences, Institute of Pharmaceutics and Biopharmaceutics, Heinrich Heine University, Universitätsstr. 1, 40225, Düsseldorf, Germany., Klassen MD; Institut Für Umwelt & Energie, Technik & Analytik E.V. (IUTA), Bliersheimer Str. 58-60, 47229, Duisburg, Germany., Fischer B; Faculty of Mathematics and Natural Sciences, Institute of Pharmaceutics and Biopharmaceutics, Heinrich Heine University, Universitätsstr. 1, 40225, Düsseldorf, Germany., Hacker MC; Faculty of Mathematics and Natural Sciences, Institute of Pharmaceutics and Biopharmaceutics, Heinrich Heine University, Universitätsstr. 1, 40225, Düsseldorf, Germany., Breitkreutz J; Faculty of Mathematics and Natural Sciences, Institute of Pharmaceutics and Biopharmaceutics, Heinrich Heine University, Universitätsstr. 1, 40225, Düsseldorf, Germany., Teutenberg T; Institut Für Umwelt & Energie, Technik & Analytik E.V. (IUTA), Bliersheimer Str. 58-60, 47229, Duisburg, Germany., Cunha R; Institut Für Umwelt & Energie, Technik & Analytik E.V. (IUTA), Bliersheimer Str. 58-60, 47229, Duisburg, Germany. cunha@iuta.de.
Πηγή:	Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2025 Aug; Vol. 417 (19), pp. 4469-4480. Date of Electronic Publication: 2025 Jun 26.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
Imprint Name(s):	Original Publication: Heidelberg : Springer-Verlag, 2002-
Ιατρικοί όροι (MeSH):	Spectrum Analysis, Raman/methods , Chromatography, Gel/methods , Antibodies, Monoclonal/analysis , Biological Products/analysis , Software*, Pharmaceutical Preparations/analysis ; Principal Component Analysis ; Workflow ; Least-Squares Analysis
Περίληψη:	Innovative hyphenated technologies, such as size exclusion chromatography coupled with capillary-enhanced Raman spectroscopy (SEC-CERS), enable more comprehensive analyses of biopharmaceutical drug products. However, specialized software for processing and analyzing data from these advanced techniques is often lacking. This study introduces the R package StreamFind, which is designed to help users seamlessly process both chromatographic and Raman spectroscopic data within an integrated workflow. We detail the implementation and structure of StreamFind and demonstrate its ability in the in-depth analysis of biopharmaceutical products. The study shows its capability to differentiate monoclonal antibodies and entire biopharmaceutical formulations and to quantify various components based on their Raman spectra. Principal component analysis (PCA) identified significant differences among the biopharmaceutical products analyzed, while multivariate curve resolution-alternating least squares (MCR-ALS) proved to be an effective method for quantifying different components within these products. The StreamFind platform is designed to be extensible, to facilitate contributions from new users and to support the future integration of additional algorithms to process different types of complex analytical data. (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Competing Interests:	Declarations. Conflict of interest: The authors declare no competing interests.
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Grant Information:	16DKWN138 Bundesministerium für Bildung und Forschung; KK5312603KA1 Bundesministerium für Wirtschaft und Klimaschutz
Contributed Indexing:	Keywords: Biopharmaceuticals; Liquid chromatography; Open-source software; Raman spectroscopy; StreamFind
Substance Nomenclature:	0 (Antibodies, Monoclonal) 0 (Biological Products) 0 (Pharmaceutical Preparations)
Entry Date(s):	Date Created: 20250626 Date Completed: 20250722 Latest Revision: 20250722
Update Code:	20260130
DOI:	10.1007/s00216-025-05964-3
PMID:	40569409
Βάση Δεδομένων:	MEDLINE

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Περιγραφή
ISSN:	1618-2650
DOI:	10.1007/s00216-025-05964-3