Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Production, optimisation and characterisation of exopolysaccharides from Aureobasidium pullulans Y428 using Melinjo seeds (Gnetum gnemon Linn.) as substrate. |
| Συγγραφείς: |
Widyastuti I; Department of Pharmaceutical Science, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia., Wikarsa S; Department of Pharmaceutical Science, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia., Soewandhi SN; Department of Pharmaceutical Science, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia., Elfahmi; Department of Pharmaceutical Science, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia.; Department of Biology Pharmacy, University Centre of Excellence for Nutraceuticals, Bioscience and Biotechnology Research Centre, Bandung Institute of Technology, Bandung, Indonesia. |
| Πηγή: |
Natural product research [Nat Prod Res] 2026 Jul; Vol. 40 (14), pp. 4222-4231. Date of Electronic Publication: 2025 May 31. |
| Τύπος έκδοσης: |
Journal Article |
| Γλώσσα: |
English |
| Στοιχεία περιοδικού: |
Publisher: Taylor & Francis Health Sciences Country of Publication: England NLM ID: 101167924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1478-6427 (Electronic) Linking ISSN: 14786419 NLM ISO Abbreviation: Nat Prod Res Subsets: MEDLINE |
| Imprint Name(s): |
Original Publication: Milton Park, UK : Taylor & Francis Health Sciences, c2003- |
| Ιατρικοί όροι (MeSH): |
Seeds*/chemistry , Seeds*/microbiology , Aureobasidium*/chemistry , Aureobasidium*/metabolism , Gnetum*/chemistry , Fungal Polysaccharides*/chemistry , Fungal Polysaccharides*/biosynthesis , Fungal Polysaccharides*/isolation & purification, Chromatography, High Pressure Liquid ; Spectroscopy, Fourier Transform Infrared ; X-Ray Diffraction ; Molecular Weight ; Microscopy, Electron, Scanning ; Calorimetry, Differential Scanning ; Thermogravimetry |
| Περίληψη: |
Exopolysaccharides (EPS) are multifunctional biopolymers with applications in the pharmaceutical, food, and cosmetic industries due to its bioactivity characteristics. This study screened strains capable of producing EPS Y428, which was isolated from Piper betle stem and identified as Aureobasidium pullulans based on its internally transcribed spacer (ITS) analysis, as well as its characteristics, colony morphology, and structure. Fourier-transform infra-red spectroscopy (FTIR), liquid chromatography-high-resolution mass spectrometry (LC-HRMS), and high-performance liquid chromatography (HPLC) confirmed the presence of a trisaccharide composed of glucose and fructose, molecular weight of 2.3 × 104 Da. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) established EPS stability at 219.32 °C, while X-ray diffraction (XRD) revealed a semi-crystalline structure, and scanning electron microscopy (SEM) demonstrated a smooth, rigid layered structure. These findings indicate that Y428 has promising potential to support global efforts in industrial innovation. |
| Contributed Indexing: |
Keywords: Aureobasidium pullulans; LC-HRMS; exopolysaccharide; fermentation; gnetum gnemon; melinjo seeds |
| Substance Nomenclature: |
0 (Fungal Polysaccharides) |
| SCR Organism: |
Aureobasidium pullulans |
| Entry Date(s): |
Date Created: 20250531 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: |
20260714 |
| DOI: |
10.1080/14786419.2025.2509880 |
| PMID: |
40449537 |
| Βάση Δεδομένων: |
MEDLINE |