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Dynamic Viscosity of Hemoglobin Solutions Determined by Transverse Proton Magnetic Relaxation.

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Τίτλος: Dynamic Viscosity of Hemoglobin Solutions Determined by Transverse Proton Magnetic Relaxation.
Συγγραφείς: Rodríguez de la Cruz NJ; Escuela de Física, Facultad de Ciencias, Universidad Autónoma de Santo Domingo, Zona Universitaria, Santo Domingo, Dominican Republic., Mengana Torres Y; Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba, Cuba., García Naranjo JC; Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba, Cuba., Ricardo Ferro BT; Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba, Cuba., Alonso Geli Y; Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba, Cuba., Guerrero Piña E; Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Santiago de Cuba, Cuba., Araujo Durán Y; Laboratorio Clínico, Hospital General Docente Dr. Juan Bruno Zayas Alfonso, Santiago de Cuba, Cuba., Suárez Beyries LC; Servicio de Hematología, Hospital General Docente Dr. Juan Bruno Zayas Alfonso, Santiago de Cuba, Cuba., Rodríguez Reyes IC; Servicio de Hematología, Hospital General Docente Dr. Juan Bruno Zayas Alfonso, Santiago de Cuba, Cuba., Rosales Rodríguez SJ; Servicio de Hematología, Hospital General Docente Dr. Juan Bruno Zayas Alfonso, Santiago de Cuba, Cuba., Guevara M; Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba, Cuba.
Πηγή: Hemoglobin [Hemoglobin] 2025 May; Vol. 49 (3), pp. 172-177. Date of Electronic Publication: 2025 Apr 24.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Informa Healthcare Country of Publication: England NLM ID: 7705865 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-432X (Electronic) Linking ISSN: 03630269 NLM ISO Abbreviation: Hemoglobin Subsets: MEDLINE
Imprint Name(s): Publication: London : Informa Healthcare
Original Publication: New York, Dekker.
Ιατρικοί όροι (MeSH): Hemoglobins*/chemistry , Hemoglobins*/analysis , Blood Viscosity*, Anemia, Sickle Cell/blood ; Humans ; Protons ; Viscosity
Περίληψη: A method based on transverse proton magnetic relaxation to determine the ηHb is presented. The procedure is supported by the inverse relationship between ηHb and T2. The Hb samples were obtained starting from whole blood of healthy individuals and patients, which was processed by classical methods (centrifugation, decanting and freezing-thawing cycles). An Ostwald's viscometer was used to measure (293 K) ηHb in Hb solutions of different concentrations and in non-diluted Hb samples belonging to healthy individuals. The CPMG pulse sequence was employed to determine T2 in a Tecmag Magnetic Resonance console coupled to a magnet of 0.095 T, and the temperature of measurement was 293 K. A calibration curve of R2 = 1/T2 as a function of ηHb was obtained, making possible the evaluation of this viscosity starting from the experimental measurement of T2. A theoretical expression was derived, which properly describes the behavior of R2 as a function of ηHb and supports the obtained calibration curve. The method developed, using the transverse proton magnetic relaxation, was successfully used to calculate ηHb in samples belonging to 10 healthy individuals, and its potential utility for medical applications was observed estimating ηHb in samples belonging to 46 sickle cell disease patients. To use this method a special care must be taken with the temperature, the value of τ and the homogeneity of the static magnetic system. Additionally, the presence inside the sample of an external amount of water, paramagnetic compounds, and/or other biological materials must be avoided.
Contributed Indexing: Keywords: Dynamic viscosity; hemoglobin solution; transverse proton magnetic relaxation
Substance Nomenclature: 0 (Hemoglobins)
0 (Protons)
Entry Date(s): Date Created: 20250425 Date Completed: 20250624 Latest Revision: 20250624
Update Code: 20260130
DOI: 10.1080/03630269.2025.2493949
PMID: 40275563
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1532-432X
DOI:10.1080/03630269.2025.2493949