Academic Journal
Multi-Omics Analyses Reveal the Red and Far-Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana.
| Τίτλος: | Multi-Omics Analyses Reveal the Red and Far-Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana. |
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| Συγγραφείς: | Zhang Y; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China., Wang F; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China., Du R; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China., Li T; Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agriculture Sciences, Beijing, China., Zhou S; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China., Yan J; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China., Li W; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. |
| Πηγή: | Plant, cell & environment [Plant Cell Environ] 2026 Oct; Vol. 49 (10), pp. 7435-7448. Date of Electronic Publication: 2025 Apr 23. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 9309004 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-3040 (Electronic) Linking ISSN: 01407791 NLM ISO Abbreviation: Plant Cell Environ Subsets: MEDLINE |
| Imprint Name(s): | Publication: Hoboken, NJ : John Wiley & Sons Ltd. Original Publication: Oxford, UK : Blackwell Scientific Publications |
| Ιατρικοί όροι (MeSH): | Nicotiana*/metabolism , Nicotiana*/radiation effects , Nicotiana*/genetics , Nicotiana*/growth & development , Light*, Green Fluorescent Proteins/metabolism ; Green Fluorescent Proteins/genetics ; Recombinant Proteins/metabolism ; Plant Leaves/metabolism ; Plant Leaves/radiation effects ; Gene Expression Regulation, Plant/radiation effects ; Plant Proteins/metabolism ; Red Light ; Multiomics ; Plants, Genetically Modified ; Proteomics ; Blue Light |
| Περίληψη: | Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of transient expression remains unexplored. In this study, we examined the influence of five representative light qualities with varying wavelength mix on the N. benthamiana growth and recombinant green fluorescent protein (GFP) production. Plants with red and far-red light treatment (LED-red) showed the highest GFP expression, 57.4% higher than white light. Further study showed that a higher dosage of post-infiltration Agrobacterium and the resulting increase in the number of transcripts contribute to the expression rate enhancement. Moreover, as for exogenous metabolites, a 76.5% increase of accumulated taxadiene was also observed in LED-red group. Integrated transcriptomic, proteomic and metabolomic revealed that LED-red plants reduced the resistance pathways before infiltration, inducing a higher dosage of post-agroinfiltration Agrobacterium. Our results suggest that N. benthamiana grown under LED-red creates a more favorable environment for Agrobacterium growth, enhancing heterologous protein and metabolite production. This study highlights the potential utilization of light quality as an implementable tool in plant synthetic biology. (© 2025 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.) |
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| Grant Information: | This work is supported by the National Key R&D Program of China (grant no.2023YFA0915800, 2020YFA0907900 and 2022YFD1700200), Shenzhen Science and Technology Program (grant no. GJHZ20240218114715030), National Natural Science Foundation of China (grant no. 32170264), the Innovation Program of Chinese Academy of Agricultural Sciences and the Elite Young Scientists Program of CAAS, Shenzhen Fundamental Research Program. |
| Contributed Indexing: | Keywords: Nicotiana benthamiana; agroinfiltration; expression efficiency; light quality; synthetic biology |
| Substance Nomenclature: | 147336-22-9 (Green Fluorescent Proteins) 0 (Recombinant Proteins) 0 (Plant Proteins) |
| Entry Date(s): | Date Created: 20250424 Date Completed: 20260908 Latest Revision: 20260910 |
| Update Code: | 20260910 |
| PubMed Central ID: | PMC13551632 |
| DOI: | 10.1111/pce.15573 |
| PMID: | 40269597 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1365-3040 |
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| DOI: | 10.1111/pce.15573 |