Academic Journal
The urease E subunit vaccine stimulate the immune response versus Helicobacter pylori in animal model.
| Τίτλος: | The urease E subunit vaccine stimulate the immune response versus Helicobacter pylori in animal model. |
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| Συγγραφείς: | Nikzad-Chaleshtori M; Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Asgari M; Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Rezaeizadeh G; Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran., Aali F; Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Doosti A; Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. doostiabbas2021@gmail.com. |
| Πηγή: | Immunologic research [Immunol Res] 2025 Apr 22; Vol. 73 (1), pp. 74. Date of Electronic Publication: 2025 Apr 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Humana Press Country of Publication: United States NLM ID: 8611087 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-0755 (Electronic) Linking ISSN: 0257277X NLM ISO Abbreviation: Immunol Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totawa Nj : Humana Press Original Publication: Basel ; New York : S. Karger, [c1986- |
| Ιατρικοί όροι (MeSH): | Helicobacter pylori*/immunology , Helicobacter pylori*/physiology , Helicobacter Infections*/immunology , Helicobacter Infections*/prevention & control , Urease*/genetics , Urease*/immunology , Vaccines, DNA*/immunology , Vaccines, DNA*/genetics , Bacterial Vaccines*/immunology , Bacterial Vaccines*/genetics , Bacterial Proteins*/genetics , Bacterial Proteins*/immunology, Antibodies, Bacterial/blood ; Vaccines, Subunit/immunology ; Cytokines/metabolism ; Cytokines/blood ; Immunoglobulin G/blood ; Animals ; Mice ; Mice, Inbred BALB C ; Disease Models, Animal ; Female ; Humans |
| Περίληψη: | There is a strong association between Helicobacter pylori (H. pylori) and the occurrence of gastritis and gastric mucosal lymphoma in the human population. Vaccination is a viable preventive measure in light of the escalating issue of antibiotic resistance. The use of DNA vaccines presents a potentially effective approach. This study used the utilization of antigenic H. pylori urease E subunit (UreE) for the development of a DNA vaccine. The UreE gene was chemically cloned into pIRES2-DsRed-Express (pDNA), and PCR and restriction enzyme digestion verified the cloning. The immunogenicity and immune-protective efficacy of the vaccination were assessed in BALB/c mice. In contrast, blood samples from BALB/c mice inoculated with pDNA-UreE showed higher levels of IgG, IFN-γ, IL- 4, and IL- 17. Furthermore, stomach damage and bacterial loads were reduced, and BALB/c mice inoculated with pDNA-UreE exhibited a significant protection rate (87.5%) against the H. pylori challenge. pDNA-UreE generated a combination of Th1-Th2-Th17 immune responses, perhaps contributing to adequate protection. Based on our findings, using this DNA immunization as a preventive measure against H. pylori infection is a viable approach. (© 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Competing Interests: | Declarations. Consent for publication: All authors agree to publish. Ethics approval: This article does not contain any studies with human participants. The study was approved by the Ethics Committee of the Islamic Azad University of Shahrekord Branch in Iran (IR.IAU.SHK.REC.1401). Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: Helicobacter pylori; DNA vaccine; Urease E subunit |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Vaccines, DNA) 0 (Bacterial Vaccines) 0 (Antibodies, Bacterial) 0 (Vaccines, Subunit) 0 (Bacterial Proteins) 0 (Cytokines) 0 (Immunoglobulin G) |
| Entry Date(s): | Date Created: 20250421 Date Completed: 20250421 Latest Revision: 20261008 |
| Update Code: | 20261008 |
| DOI: | 10.1007/s12026-025-09625-6 |
| PMID: | 40259189 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1559-0755 |
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| DOI: | 10.1007/s12026-025-09625-6 |