Academic Journal
Investigation of the inhibitory effects of immunoglobulin Y antibody against key epitopes of Helicobacter pylori UreB recombinant protein.
| Τίτλος: | Investigation of the inhibitory effects of immunoglobulin Y antibody against key epitopes of Helicobacter pylori UreB recombinant protein. |
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| Συγγραφείς: | Esmaeili Z; Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Kamal Shahsavar S; Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Ariannejad H; Institute of Biotechnology, Ferdowsi University of Mashhad, Iran., Hajinajaf N; Chemical Engineering Program, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ, USA., Menbari S; Department of Medical Laboratory Sciences, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran. Electronic address: shahoo1020@gmail.com., Ghazvini K; Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: GhazviniK@mums.ac.ir. |
| Πηγή: | Microbial pathogenesis [Microb Pathog] 2025 Jul; Vol. 204, pp. 107613. Date of Electronic Publication: 2025 Apr 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London ; Orlando : Academic Press, c1986- |
| Ιατρικοί όροι (MeSH): | Helicobacter pylori*/immunology , Helicobacter pylori*/genetics , Helicobacter pylori*/growth & development , Helicobacter pylori*/drug effects , Antibodies, Bacterial*/immunology , Antibodies, Bacterial*/isolation & purification , Epitopes*/immunology , Epitopes*/genetics , Urease*/immunology , Urease*/genetics , Bacterial Proteins*/immunology , Bacterial Proteins*/genetics , Immunoglobulins*/immunology , Immunoglobulins*/isolation & purification, Recombinant Proteins/immunology ; Recombinant Proteins/genetics ; Helicobacter Infections/immunology ; Helicobacter Infections/microbiology ; Escherichia coli/genetics ; Virulence Factors/immunology ; Animals ; Chickens ; Female ; Cloning, Molecular |
| Περίληψη: | Helicobacter pylori (H.pylori) is considered to be the most important gastrointestinal pathogen causing gastritis, gastric ulcers and even gastric cancer. The treatment of these infections has failed due to the rapidly increasing antibiotic resistance to standard treatment regimens and the lack of an effective vaccine. This study investigates the production and therapeutic potential of Immunoglobulin Y (IgY) antibodies targeting key epitopes of the H. pylori UreB recombinant protein. Given the increasing challenge of antibiotic resistance in H. pylori treatment, this research underscores the necessity for alternative therapeutic strategies. A specific region of the UreB gene, containing critical immunogenic epitopes, was amplified using Polymerase Chain Reaction (PCR) and cloned into the pET32b vector. The recombinant plasmid was expressed in Escherichia coli BL21 (DE3), and the UreB protein was purified via Ni-NTA affinity chromatography, confirmed by SDS-PAGE and Western blot analysis. Hens were immunized with the recombinant UreB protein, resulting in the generation of specific IgY antibodies. The purified IgY-UreB antibodies exhibited a remarkable reduction in urease activity by 84.53 % at a concentration of 10 mg/mL, effectively neutralizing this critical virulence factor. Additionally, in vitro assays demonstrated that IgY-UreB antibodies significantly inhibited the growth of H. pylori at a concentration of 5 mg/mL. These findings highlight the potential of IgY as a viable alternative to traditional antibiotic therapies, particularly in the context of rising antibiotic resistance. This study paves the way for the development of innovative immunotherapeutic strategies that may improve treatment outcomes for H. pylori infections. (Copyright © 2025 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Kiarash Ghazvini reports financial support was provided by Mashhad University of Medical Sciences. Shaho Menbari reports statistical analysis was provided by Kurdistan University of Medical Sciences. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Gastritis; Gene cloning; Helicobacter pylori; IgY antibodies; Multi epitope; Urease B |
| Substance Nomenclature: | 0 (Recombinant Proteins) 0 (Antibodies, Bacterial) 0 (Epitopes) EC 3.5.1.5 (Urease) 0 (IgY) 0 (Bacterial Proteins) 0 (Immunoglobulins) 0 (Virulence Factors) |
| Entry Date(s): | Date Created: 20250419 Date Completed: 20250511 Latest Revision: 20250511 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.micpath.2025.107613 |
| PMID: | 40252938 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2025.107613 |