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Fur-regulated urease contributes to the environmental adaptation of Yersinia pseudotuberculosis.

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Τίτλος: Fur-regulated urease contributes to the environmental adaptation of Yersinia pseudotuberculosis.
Συγγραφείς: Wang J; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Fu P; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., He X; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Liu Y; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Zuo Y; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Wei Z; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Wang Y; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Yang Y; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Li C; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Shen X; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China., Zhu L; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Πηγή: Microbiology spectrum [Microbiol Spectr] 2025 Apr; Vol. 13 (4), pp. e0275624. Date of Electronic Publication: 2025 Feb 25.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, DC : ASM Press, 2013-
Ιατρικοί όροι (MeSH): Urease*/metabolism , Urease*/genetics , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics , Yersinia pseudotuberculosis*/genetics , Yersinia pseudotuberculosis*/enzymology , Yersinia pseudotuberculosis*/pathogenicity , Yersinia pseudotuberculosis*/metabolism , Yersinia pseudotuberculosis*/physiology , Repressor Proteins*/metabolism , Repressor Proteins*/genetics, Manganese/metabolism ; Biofilms/growth & development ; Yersinia pseudotuberculosis Infections/microbiology ; Gene Expression Regulation, Bacterial ; Virulence ; Promoter Regions, Genetic ; Adaptation, Physiological ; Animals
Περίληψη: Urease converts urea into ammonia and carbon dioxide, providing a nitrogen and carbon source for microbial growth and serving as an important mechanism for human bacterial pathogens to survive in acidic conditions, which can be regulated by many factors. As a global regulator, the ferric uptake regulator (Fur) regulates a series of genes and pathways involved in many different cellular processes and the virulence of the enteric bacterium Yersinia pseudotuberculosis (Yptb). However, whether Fur regulates the urease activity in Yptb was still unknown. In this study, we found that urease is positively regulated by Fur in response to manganese ions (Mn2+), and this regulation by Fur is mediated by specific recognition of the promoter region of urease in Yptb. Furthermore, urease is induced by Mn2+ via Fur under low nutrient conditions. Moreover, we provided evidence that urease plays an important role in acid and osmotic stress resistance, biofilm formation, and virulence of Yptb. Our findings provide insights into understanding the regulatory mechanism and multiple functions of urease in Yptb.IMPORTANCEUrease catalyzes the breakdown of urea into ammonia and carbamate, which are widely distributed among bacterial species and play an important role as an important acid resistance system and virulence factor. In most bacterial species, urease expression is tightly regulated in response to environmental cues such as nitrogen status, pH, growth phase, substrate availability, or transcriptional regulators. In this study, we found that urease from Yptb is positively regulated by Fur in response to Mn2+ under low nutrient conditions, which functions to combat acid and osmotic stress and enhance biofilm formation, and plays a crucial role in virulence. Importantly, this is the first demonstration of a direct role for Fur and Mn2+ in regulating urease expression in Yptb. This study provides a comprehensive understanding of the regulatory mechanisms and functions of urease from Yptb.
Competing Interests: The authors declare no conflict of interest.
References: Front Microbiol. 2020 Apr 08;11:607. (PMID: 32322248)
mBio. 2013 May 07;4(3):e00220-13. (PMID: 23653445)
Stress Biol. 2022 Dec 28;2(1):55. (PMID: 37676573)
Front Microbiol. 2021 Jul 15;12:699913. (PMID: 34335534)
Infect Immun. 1997 May;65(5):1985-90. (PMID: 9125594)
FEMS Yeast Res. 2020 Jun 1;20(4):. (PMID: 32490521)
Nat Rev Microbiol. 2016 Aug 11;14(9):563-75. (PMID: 27510863)
Microbiol Spectr. 2023 Mar 14;:e0350822. (PMID: 36916906)
Infect Immun. 2005 Oct;73(10):6437-45. (PMID: 16177315)
J Pathog. 2011;2011:182051. (PMID: 22567322)
J Appl Microbiol. 2004;96(1):209-19. (PMID: 14678176)
Nat Commun. 2020 Oct 9;11(1):5101. (PMID: 33037208)
J Bacteriol. 1996 Nov;178(22):6487-95. (PMID: 8932305)
Microbiol Rev. 1989 Mar;53(1):85-108. (PMID: 2651866)
Cell Host Microbe. 2023 Nov 8;31(11):1820-1836.e10. (PMID: 37848028)
Infect Immun. 1991 Jul;59(7):2470-5. (PMID: 2050411)
PLoS Pathog. 2014 Dec 11;10(12):e1004472. (PMID: 25501953)
Infect Immun. 1995 Oct;63(10):3790-5. (PMID: 7558281)
Adv Genet. 2022;109:39-69. (PMID: 36334916)
Front Microbiol. 2018 Feb 27;9:348. (PMID: 29535702)
Helicobacter. 2011 Jun;16(3):189-99. (PMID: 21585604)
mBio. 2015 Dec 15;6(6):e01947-15. (PMID: 26670385)
J Biol Chem. 2013 Nov 1;288(44):31503-16. (PMID: 24043617)
BMC Syst Biol. 2017 Jan 6;11(1):1. (PMID: 28061857)
Yonsei Med J. 2010 Jan;51(1):39-44. (PMID: 20046512)
PLoS Pathog. 2019 Jan 4;15(1):e1007538. (PMID: 30608981)
Infect Immun. 2002 Feb;70(2):1027-31. (PMID: 11796646)
Proc Natl Acad Sci U S A. 2021 Oct 19;118(42):. (PMID: 34625471)
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9542-7. (PMID: 12883001)
Biomolecules. 2024 Aug 09;14(8):. (PMID: 39199369)
Infect Immun. 2007 Feb;75(2):745-52. (PMID: 17101656)
New Microbes New Infect. 2019 Oct 07;32:100604. (PMID: 31719995)
Microbiology (Reading). 2009 Aug;155(Pt 8):2522-2531. (PMID: 19443542)
Curr Protein Pept Sci. 2012 Dec;13(8):789-806. (PMID: 23305365)
Stress Biol. 2023 Jan 6;3(1):2. (PMID: 37676351)
Infect Immun. 2001 Aug;69(8):4891-7. (PMID: 11447165)
Front Cell Infect Microbiol. 2018 Sep 18;8:323. (PMID: 30280093)
J Biotechnol. 2006 Oct 20;126(1):52-60. (PMID: 16713649)
Biosens Bioelectron. 2015 May 15;67:465-71. (PMID: 25223554)
Cell Host Microbe. 2015 Aug 12;18(2):147-56. (PMID: 26269952)
Arch Biochem Biophys. 2014 Mar 15;546:41-52. (PMID: 24513162)
Appl Environ Microbiol. 2004 Apr;70(4):2342-8. (PMID: 15066830)
Trends Microbiol. 2002 Feb;10(2):70-4. (PMID: 11827807)
J Adv Res. 2018 May 28;13:3-17. (PMID: 30094078)
J Microbiol Immunol Infect. 2022 Aug;55(4):620-633. (PMID: 35367140)
Int J Mol Sci. 2016 Mar 11;17(3):363. (PMID: 26978353)
Nat Commun. 2022 Dec 16;13(1):7779. (PMID: 36522324)
Front Cell Infect Microbiol. 2013 Oct 02;3:59. (PMID: 24106689)
Clin Microbiol Rev. 2002 Apr;15(2):167-93. (PMID: 11932229)
mBio. 2011 Apr 19;2(2):e00039-11. (PMID: 21505055)
Infect Immun. 2007 Feb;75(2):774-80. (PMID: 17101645)
J Bacteriol. 2000 May;182(9):2649-53. (PMID: 10762273)
ISME J. 2022 Feb;16(2):500-510. (PMID: 34433898)
Mol Microbiol. 1993 Sep;9(5):907-13. (PMID: 7934918)
Environ Microbiol. 2015 Nov;17(11):4631-45. (PMID: 26234561)
PLoS Pathog. 2018 Feb 1;14(2):e1006858. (PMID: 29390040)
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11978-82. (PMID: 1465428)
Grant Information: 32470039, 32100149 MOST | National Natural Science Foundation of China (NSFC); 32330004 MOST | National Natural Science Foundation of China (NSFC); 32470197, 32100034 MOST | National Natural Science Foundation of China (NSFC); 20220206 Young Talent Support Program of Shaanxi Province University
Contributed Indexing: Keywords: Fur; Mn2+; Yersinia pseudotuberculosis; urease
Substance Nomenclature: EC 3.5.1.5 (Urease)
0 (Bacterial Proteins)
42Z2K6ZL8P (Manganese)
0 (Repressor Proteins)
0 (ferric uptake regulating proteins, bacterial)
Entry Date(s): Date Created: 20250225 Date Completed: 20250509 Latest Revision: 20250509
Update Code: 20260130
PubMed Central ID: PMC11960103
DOI: 10.1128/spectrum.02756-24
PMID: 39998249
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2165-0497
DOI:10.1128/spectrum.02756-24