Academic Journal
A novel urease gene structure of Sporosarcina pasteurii with double operons.
| Τίτλος: | A novel urease gene structure of Sporosarcina pasteurii with double operons. |
|---|---|
| Συγγραφείς: | Pei D; College of Sciences, National University of Defense Technology, Changsha, Hunan, China. 397141499@qq.com.; China Astronaut Research and Training Center, Beijing, China. 397141499@qq.com., Liu Z; Academy of Chemical Defense, Academy of Military Science, Beijing, China., Hu B; College of Sciences, National University of Defense Technology, Changsha, Hunan, China. |
| Πηγή: | Molecular genetics and genomics : MGG [Mol Genet Genomics] 2025 Feb 22; Vol. 300 (1), pp. 25. Date of Electronic Publication: 2025 Feb 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101093320 Publication Model: Electronic Cited Medium: Internet ISSN: 1617-4623 (Electronic) Linking ISSN: 16174623 NLM ISO Abbreviation: Mol Genet Genomics Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin : Springer-Verlag, c2001- |
| Ιατρικοί όροι (MeSH): | Urease*/genetics , Urease*/metabolism , Sporosarcina*/genetics , Sporosarcina*/enzymology , Sporosarcina*/growth & development , Operon*/genetics , Bacterial Proteins*/genetics, Calcium Carbonate/metabolism ; Promoter Regions, Genetic ; Gene Expression Regulation, Bacterial ; Transcription Initiation Site |
| Περίληψη: | Microbially induced calcium carbonate precipitation (MICP) had emerged as an important biomineralization process with wide-ranging applications in construction, environmental remediation, and space exploration. Sporosarcina pasteurii (S. pasteurii) was a key bacterium in MICP due to its efficient urease activity, yet the regulation of its urease genes remains poorly understood, limiting its practical applications. This study aimed to elucidate the structure and expression regulation mechanism of urease genes in S. pasteurii to enhance its mineralization potential. We compared the growth and urease gene expression of S. pasteurii under three different culture conditions using transcriptome sequencing. Operon, Transcription Start Site (TSS) and Transcription Termination Site (TTS) were predicted based on the distribution of reads on the genome using Rockhopper online analysis software. The 700 bp sequence upstream of the TTS was extracted and promoter prediction was performed by Time-Delay Neural Network (TDNN) method. Finally, we verified the prediction results by RT-PCR. Our results revealed, for the first time, a double operon structure of S. pasteurii urease, with operon 1 containing ureA, ureB, ureC, ureE, and ureF genes, and operon 2 containing ureG and ureD genes. This discovery provides crucial insights into the regulation of urease expression in S. pasteurii, paving the way for more efficient and controllable mineralization applications. The findings of this study not only advanced our understanding of urease gene regulation but also opened new avenues for optimizing S. pasteurii-based biomineralization technologies. (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| Competing Interests: | Declarations. Competing interests: The authors had declared that no competing interests exist. |
| References: | Curr Opin Microbiol. 2005 Apr;8(2):203-7. (PMID: 15802253) J Bacteriol. 2003 Mar;185(6):1911-22. (PMID: 12618455) J Bacteriol. 1990 Dec;172(12):7249-55. (PMID: 1979323) Mol Microbiol. 1995 Apr;16(1):97-109. (PMID: 7651142) Genes Dev. 1995 Aug 15;9(16):2042-52. (PMID: 7649482) Nucleic Acids Res. 2013 Aug;41(14):e140. (PMID: 23716638) Infect Immun. 2005 Nov;73(11):7252-8. (PMID: 16239520) J Bacteriol. 1993 Jan;175(2):465-73. (PMID: 7678244) Appl Environ Microbiol. 2014 Sep;80(17):5386-93. (PMID: 24951785) Mol Microbiol. 2000 Jun;36(5):1071-84. (PMID: 10844692) J Bacteriol. 1995 Jun;177(12):3546-55. (PMID: 7768865) J Clin Microbiol. 2006 Sep;44(9):3429-31. (PMID: 16954293) J Bacteriol. 1997 May;179(10):3371-3. (PMID: 9150240) J Bacteriol. 2005 Oct;187(20):7150-4. (PMID: 16199586) J Bacteriol. 1997 Sep;179(17):5494-501. (PMID: 9287005) Infect Immun. 2005 Oct;73(10):6437-45. (PMID: 16177315) J Biotechnol. 2012 Oct 31;161(3):181-9. (PMID: 22789480) |
| Contributed Indexing: | Keywords: Sporosarcina pasteurii urease; Double operons; Gene structure; MICP |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Bacterial Proteins) H0G9379FGK (Calcium Carbonate) |
| SCR Organism: | Sporosarcina pasteurii |
| Entry Date(s): | Date Created: 20250222 Date Completed: 20250509 Latest Revision: 20261010 |
| Update Code: | 20261010 |
| DOI: | 10.1007/s00438-025-02236-8 |
| PMID: | 39985601 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1617-4623 |
|---|---|
| DOI: | 10.1007/s00438-025-02236-8 |