Academic Journal
Electrical Stimulation Attenuates Muscle Atrophy Induced by Brachial Plexus Injury by Upregulating HO-1 Expression in Rats.
| Τίτλος: | Electrical Stimulation Attenuates Muscle Atrophy Induced by Brachial Plexus Injury by Upregulating HO-1 Expression in Rats. |
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| Συγγραφείς: | Qin H; From the Departments of Hand and Foot Surgery., Du X; Electrophysiology, Yijishan Hospital of Wannan Medical College., Hu B; From the Departments of Hand and Foot Surgery., Ma T; From the Departments of Hand and Foot Surgery. |
| Πηγή: | Plastic and reconstructive surgery [Plast Reconstr Surg] 2025 Sep 01; Vol. 156 (3), pp. 366e-374e. Date of Electronic Publication: 2025 Feb 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 1306050 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1529-4242 (Electronic) Linking ISSN: 00321052 NLM ISO Abbreviation: Plast Reconstr Surg Subsets: MEDLINE |
| Imprint Name(s): | Publication: Original Publication: Baltimore : Williams & Wilkins |
| Ιατρικοί όροι (MeSH): | Muscular Atrophy*/etiology , Muscular Atrophy*/therapy , Muscular Atrophy*/prevention & control , Electric Stimulation Therapy*/methods , Brachial Plexus*/injuries , Heme Oxygenase-1*/metabolism , Brachial Plexus Neuropathies*/complications , Brachial Plexus Neuropathies*/therapy, Muscle, Skeletal/innervation ; Muscle, Skeletal/pathology ; Muscle, Skeletal/metabolism ; Muscle Denervation/adverse effects ; Electric Stimulation/methods ; Animals ; Rats ; Up-Regulation ; Disease Models, Animal ; Male ; Rats, Sprague-Dawley ; Heme Oxygenase (Decyclizing) |
| Περίληψη: | Background: In cases of global brachial plexus injury, long-term denervation results in severe muscle atrophy in the hand. This study was conducted to investigate the protective effect of electrical stimulation (ES) on denervated intrinsic forepaw muscle (IFM) induced by global brachial plexus avulsion (GBPA) in rats, and whether heme oxygenase-1 (HO-1) is involved in the effect of ES on denervated IFM atrophy. Methods: The authors established a GBPA rat model and denervated IFM was electrically stimulated at different frequencies (2, 10, and 20 Hz). Muscle fiber cross-sectional area, capillary supply, and muscular HO-1 protein level were measured. In addition, the expression of HO-1 was locally blocked by injecting the small interfering (si) RNA into IFM to determine the possible role of HO-1 in ES antiatrophic effect. Several key regulators of muscle protein synthesis and degradation were determined. Results: All the stimulation protocols were able to ameliorate muscle capillary loss after denervation. ES at 20 Hz significantly attenuated denervated IFM atrophy. The protective effects of ES were markedly weakened by local injection of the HO-1 siRNA. Meanwhile, the expressions of MuRF-1, MAFbx, BNIP3, and the LC3B-II/I ratio were upregulated by denervation, whereas ES treatment attenuated the increment. Low muscular HO-1 level almost completely suppressed the ability of ES to inhibit the hydrolysis activity of autophagy-lysosomal pathway. Conclusions: ES at 20 Hz, using a clinically translatable protocol, is recommended to delay denervated IFM atrophy. Activation of HO-1 is an important mechanism involved in the protective effects of ES against denervation-related muscle atrophy. Clinical Relevance Statement: Muscular HO-1 may be a potential molecular marker for the instruction and optimization of stimulus settings. Muscle-targeted upregulation of HO-1 may be a promising strategy for retarding denervation-related muscle atrophy. (Copyright © 2025 by the American Society of Plastic Surgeons.) |
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| Substance Nomenclature: | EC 1.14.14.18 (Hmox1 protein, rat) EC 1.14.14.18 (Heme Oxygenase-1) EC 1.14.14.18 (Heme Oxygenase (Decyclizing)) |
| Entry Date(s): | Date Created: 20250213 Date Completed: 20250828 Latest Revision: 20260511 |
| Update Code: | 20260511 |
| DOI: | 10.1097/PRS.0000000000012025 |
| PMID: | 39946663 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1529-4242 |
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| DOI: | 10.1097/PRS.0000000000012025 |