Academic Journal
Portable pH meter-based competitive immunoassay of E-selectin using urease-encapsulated metal-organic frameworks.
| Τίτλος: | Portable pH meter-based competitive immunoassay of E-selectin using urease-encapsulated metal-organic frameworks. |
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| Συγγραφείς: | Xie Y; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China., He X; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China., Yu R; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China., Jin Y; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China., Tan L; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China. Electronic address: liangtan@hunnu.edu.cn. |
| Πηγή: | Talanta [Talanta] 2025 May 15; Vol. 287, pp. 127613. Date of Electronic Publication: 2025 Jan 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| Ιατρικοί όροι (MeSH): | Urease*/chemistry , Urease*/metabolism , Metal-Organic Frameworks*/chemistry , Enzymes, Immobilized*/chemistry , Enzymes, Immobilized*/metabolism , E-Selectin*/analysis , E-Selectin*/immunology, Immunoassay/methods ; Immunoassay/instrumentation ; Nanocomposites/chemistry ; Hydrogen-Ion Concentration ; Humans |
| Περίληψη: | E-selectin (CD62E) is an adhesion molecule expressed on the surface of endothelial cells (ECs) and its level increases significantly upon the stimulation of ECs by inflammatory factors. Quantitative analysis of CD62E is of great importance to early diagnosis and treatment of vascular diseases and hypertension. A new method for the determination of CD62E was developed using a portable pH meter in this work. Two nanocomposites, urease-encapsulated and CD62E antibody-linked zeolitic imidazolate framework-8 (U@ZIF-8-Ab) and CD62E protein-coated Fe (Copyright © 2025 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no conflicts of interest to this work. |
| Contributed Indexing: | Keywords: Competitive immunoassay; E-Selection; Portable pH meter; Urea hydrolysis; Urease-encapsulated MOFs |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Metal-Organic Frameworks) 0 (Enzymes, Immobilized) 0 (E-Selectin) |
| Entry Date(s): | Date Created: 20250125 Date Completed: 20250503 Latest Revision: 20250524 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.talanta.2025.127613 |
| PMID: | 39862514 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2025.127613 |