Academic Journal
Curious effects of overlooked aspects on urease activity.
| Τίτλος: | Curious effects of overlooked aspects on urease activity. |
|---|---|
| Συγγραφείς: | Acar M; Department of Chemistry 'Ugo Schiff' and CSGI, University of Florence, Sesto Fiorentino, Firenze 50019, Italy., Tatini D; Department of Biotechnologies, Chemistry and Pharmacy, University of Siena, Siena 53100, Italy., Romani V; Department of Chemistry 'Ugo Schiff' and CSGI, University of Florence, Sesto Fiorentino, Firenze 50019, Italy., Ninham BW; Department of Materials Physics, Research School of Physics, Australian National University, Canberra, ACT 0200, Australia., Rossi F; Department of Earth, Environmental and Physical Sciences-DEEP Sciences, University of Siena, Siena 53100, Italy., Lo Nostro P; Department of Chemistry 'Ugo Schiff' and CSGI, University of Florence, Sesto Fiorentino, Firenze 50019, Italy. Electronic address: pierandrea.lonostro@unifi.it. |
| Πηγή: | Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2025 Mar; Vol. 247, pp. 114422. Date of Electronic Publication: 2024 Dec 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| Ιατρικοί όροι (MeSH): | Urease*/chemistry , Urease*/metabolism, Urea/chemistry ; Urea/metabolism ; Water/chemistry ; Solvents/chemistry ; Bicarbonates/chemistry ; Bicarbonates/metabolism ; Hydrogen-Ion Concentration ; Circular Dichroism ; Hydrolysis |
| Περίληψη: | Intermolecular forces determine complex chemical structures of exquisite intricacy, like proteins. However even the most advanced theories we have so far rely on too drastic approximations to explain them. Some crucial aspects that dictate structure, specific ion and solvent effects are not accommodated. Further the very significant effects of dissolved atmospheric gas are completely ignored and unexplored. Here we examine the effects of cations, dissolved gasses, and heavy water on the pH clock reactions of urease. This enzyme catalyzes the hydrolysis of urea to ammonium and bicarbonate in unbuffered aqueous solutions. In so doing it increases the pH. Circular dichroism and fluorescence experiments are used to assess conformational effects. The results highlight the subtle interplay of different factors that participate in determining the urease activity. The experimental data are correlated with specific ion physicochemical parameters and conformational data. They are explored in the context of specific ion and solvent interactions and hydration. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Chaotropicity; Dissolved gases; Enzymatic activity; Heavy water; Hofmeister series; Kosmotropicity; Urease |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 8W8T17847W (Urea) 059QF0KO0R (Water) 0 (Solvents) 0 (Bicarbonates) |
| Entry Date(s): | Date Created: 20241214 Date Completed: 20250427 Latest Revision: 20250518 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.colsurfb.2024.114422 |
| PMID: | 39673898 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-4367 |
|---|---|
| DOI: | 10.1016/j.colsurfb.2024.114422 |