Variable gain DNA nanostructure charge amplifiers for biosensing.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Variable gain DNA nanostructure charge amplifiers for biosensing.
Συγγραφείς:	Majikes JM; Microsystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. arvind.balijepalli@nist.gov., Cho S; Microsystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. arvind.balijepalli@nist.gov., Cleveland TE 4th; Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.; Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA., Liddle JA; Microsystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. arvind.balijepalli@nist.gov., Balijepalli A; Microsystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. arvind.balijepalli@nist.gov.
Πηγή:	Nanoscale [Nanoscale] 2024 Nov 21; Vol. 16 (45), pp. 20893-20902. Date of Electronic Publication: 2024 Nov 21.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: RSC Pub Country of Publication: England NLM ID: 101525249 Publication Model: Electronic Cited Medium: Internet ISSN: 2040-3372 (Electronic) Linking ISSN: 20403364 NLM ISO Abbreviation: Nanoscale Subsets: MEDLINE
Imprint Name(s):	Original Publication: Cambridge, UK : RSC Pub.
Ιατρικοί όροι (MeSH):	DNA/chemistry , Nanostructures/chemistry , Biosensing Techniques*, Nucleic Acid Hybridization ; Nucleic Acid Conformation ; Static Electricity ; Electrochemical Techniques
Περίληψη:	Electronic measurements of engineered nanostructures comprised solely of DNA (DNA origami) enable new signal conditioning modalities for use in biosensing. DNA origami, designed to take on arbitrary shapes and allow programmable motion triggered by conjugated biomolecules, have sufficient mass and charge to generate a large electrochemical signal. Here, we demonstrate the ability to electrostatically control the DNA origami conformation, and thereby the resulting signal amplification, when the structure binds a nucleic acid analyte. Critically, unlike previous studies that employ DNA origami to amplify an electrical signal, we show that the conformation changes under an applied field are reversible. This applied field also simultaneously accelerates structural transitions above the rate determined by thermal motion. We tuned this property of the structures to achieve a response that was ≈2 × 10⁴ times greater (i.e., a gain or amplification) than the value from DNA hybridization under similar conditions. Because this signal amplification is independent of DNA origami-analyte interactions, our approach is agnostic of the end application. Furthermore, since large signal changes are only triggered in response to desirable interactions, we minimize the deleterious effects of non-specific binding. The above benefits of self-assembled DNA origami make them ideally suited for multiplexed biosensing when paired with highly parallel electronic readout.
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Grant Information:	9999-NIST United States ImNIST Intramural NIST DOC
Substance Nomenclature:	9007-49-2 (DNA)
Entry Date(s):	Date Created: 20241015 Date Completed: 20241121 Latest Revision: 20251122
Update Code:	20260130
PubMed Central ID:	PMC11883816
DOI:	10.1039/d4nr02959c
PMID:	39403767
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	2040-3372
DOI:	10.1039/d4nr02959c