Academic Journal
Substitution of Asp29 with Asn29 in the metallochaperone UreE of Streptococcus thermophilus DSM 20617T increases the urease activity and anticipates urea hydrolysis during milk fermentation.
| Τίτλος: | Substitution of Asp29 with Asn29 in the metallochaperone UreE of Streptococcus thermophilus DSM 20617T increases the urease activity and anticipates urea hydrolysis during milk fermentation. |
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| Συγγραφείς: | Arioli S; Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy., Mangieri N; Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy., Zanchetta Y; Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy., Russo P; Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy., Mora D; Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy. Electronic address: diego.mora@unimi.it. |
| Πηγή: | International journal of food microbiology [Int J Food Microbiol] 2024 May 02; Vol. 416, pp. 110684. Date of Electronic Publication: 2024 Mar 16. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8412849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3460 (Electronic) Linking ISSN: 01681605 NLM ISO Abbreviation: Int J Food Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Elsevier Science Publishers, c1984- |
| Ιατρικοί όροι (MeSH): | Urease*/genetics , Streptococcus thermophilus*/metabolism, Metallochaperones/metabolism ; Carrier Proteins/genetics ; Nickel/metabolism ; Milk/metabolism ; Bacterial Proteins/genetics ; Animals ; Hydrolysis ; Urea ; Fermentation |
| Περίληψη: | Urease operon is highly conserved within the species Streptococcus thermophilus and urease-negative strains are rare in nature. S. thermophilus MIMO1, isolated from commercial yogurt, was previously characterized as urease-positive Ni-dependent strain. Beside a mutation in ureQ, coding for a nickel ABC transporter permease, the strain MIMO1 showed a mutation in ureE gene which code for a metallochaperone involved in Ni delivery to the urease catalytic site. The single base mutation in ureE determined a substitution of Asp (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest None. |
| Contributed Indexing: | Keywords: Milk acidification; Ni; Streptococcus thermophilus; UreE; Urease |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Metallochaperones) 0 (Carrier Proteins) 7OV03QG267 (Nickel) 8W8T17847W (Urea) 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20240321 Date Completed: 20240419 Latest Revision: 20240419 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.ijfoodmicro.2024.110684 |
| PMID: | 38513545 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-3460 |
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| DOI: | 10.1016/j.ijfoodmicro.2024.110684 |