Academic Journal
Structural and Functional Analysis of Urease Accessory Protein E from Vancomycin-Resistance Staphylococcus aureus MU50 Strain.
| Τίτλος: | Structural and Functional Analysis of Urease Accessory Protein E from Vancomycin-Resistance Staphylococcus aureus MU50 Strain. |
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| Συγγραφείς: | Siddiqui H; Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan., Atia-Tul-Wahab; Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan., Ahmed A; School of Pharmacy, Chapman University, Irvine, CA 92618, USA., Choudhary MI; H.E.J. Research-Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah-21412, Saudi Arabia.; Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan. |
| Πηγή: | Protein and peptide letters [Protein Pept Lett] 2023; Vol. 30 (9), pp. 754-762. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 9441434 Publication Model: Print Cited Medium: Internet ISSN: 1875-5305 (Electronic) Linking ISSN: 09298665 NLM ISO Abbreviation: Protein Pept Lett Subsets: MEDLINE |
| Imprint Name(s): | Publication: Hilversum, The Netherlands : Bentham Science Publishers Original Publication: Schiphol, The Netherlands : Bentham Science Publishers, c1994- |
| Ιατρικοί όροι (MeSH): | Urease*/genetics , Urease*/chemistry , Urease*/metabolism , Carrier Proteins*/chemistry, Vancomycin/pharmacology ; Vancomycin/metabolism ; Staphylococcus aureus/genetics ; Gentian Violet/pharmacology ; Bacterial Proteins/chemistry ; Nickel/pharmacology |
| Περίληψη: | Background: An increasing prevalence of biofilm forming strains by vancomycinresistance Staphylococcus aureus (VRSA) is one of the most important causes of antimicrobial resistance. VRSA possesses various regulatory factors to form and sustain biofilm in biotic or abiotic conditions. Among them, ureolytic activity is an important factor in the stabilization of biofilms by neutralizing the acidic environment. Various urease accessory proteins are required to activate the urease enzyme inside the biofilm. Objective: To optimize the cloning, expression and purification of urease accessory protein E from VRSA for determination of the secondary structure, and functional characterization by using Berthelot's method. Methods: BAB58453.1 gene (which encodes possible urease accessory protein E), having 38% similarity to Bacillus pasteurii UreE protein, was cloned, expressed, and purified by single-step affinity chromatography for performing secondary structural studies using circular dichroism spectroscopy, and functional analysis using Berthelot's and crystal violet assay. Results: Structure elucidation using NMR and circular dichroism spectroscopy techniques revealed that UreE protein has a partially foldedα-helical structure. Using Berthelot's method, it was identified that the purified UreE protein has enhanced urease enzyme activity, in comparison to the control. From the results of Berthelot's and crystal violet assays, it was deduced that the selected gene (UreE protein) plays a key role in enhancing urease enzyme activity and contributes to biofilm stability. Conclusion: Structural studies on VRSA urease accessory proteins could aid in the identification of new drug targets or the development of effective antibiofilm strategies (in combination with other drug targets) against infections caused by biofilm-producing strains. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| Contributed Indexing: | Keywords: NMR.; Staphylococcus aureus; Urease accessory protein E; VRSA; biofilm; urease enzyme |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Carrier Proteins) 6Q205EH1VU (Vancomycin) J4Z741D6O5 (Gentian Violet) 0 (Bacterial Proteins) 7OV03QG267 (Nickel) |
| Entry Date(s): | Date Created: 20230803 Date Completed: 20231127 Latest Revision: 20231127 |
| Update Code: | 20260130 |
| DOI: | 10.2174/0929866530666230801163340 |
| PMID: | 37533244 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1875-5305 |
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| DOI: | 10.2174/0929866530666230801163340 |