Academic Journal
Molecular characterization of Trypanosoma evansi, T. vivax and T. congolense in camels (Camelus dromedarius) of KSA.
| Τίτλος: | Molecular characterization of Trypanosoma evansi, T. vivax and T. congolense in camels (Camelus dromedarius) of KSA. |
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| Συγγραφείς: | Al Malki JS; Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia., Hussien NA; Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia. n.nahed@tu.edu.sa. |
| Πηγή: | BMC veterinary research [BMC Vet Res] 2022 Jan 18; Vol. 18 (1), pp. 45. Date of Electronic Publication: 2022 Jan 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, 2005- |
| Ιατρικοί όροι (MeSH): | Trypanosoma*/classification , Trypanosoma*/genetics , Trypanosomiasis*/epidemiology , Trypanosomiasis*/veterinary, Camelus/*parasitology, Animals ; Female ; Male ; Phylogeny ; Saudi Arabia |
| Περίληψη: | Background: Trypanosoma evansi is the leading infectious Trypanosoma spp. in camels (Camelus dromedarius) present in the Kingdom of Saudi Arabia (KSA) that could lead to extensive economic losses. The present study was aimed to assess the prevalence rate of T. evansi in Taif governorate, Makkah province, KSA using parasitological and molecular evaluations, and analyze their genetic relationship targeting internal transcribed spacer 1 (ITS1) and variable surface glycoprotein (VSG) genes. For evaluation, we have used 102 blood samples of camels obtained from three different regions in Taif. Results: Results show a considerable prevalence rate of trypanosomosis 2/102 (2.0%) according to Giemsa-stained buffy coat smear, and 16/102 (15.7%) according to touchdown PCR. T. evansi (n = 10/102, 9.8%) was the main infectious species found in camels then T. vivax (n = 3/102, 2.9%). Mixed infections were detected in three camels with T. evansi, T. vivax, and T. congolense (n = 3/102, 2.9%). Regarding gender, the results indicate that female camels (11/66, 16.7%) show higher prevalence of Trypanosoma than males (5/36, 13.9%). Sequencing and phylogenetic analyses of ITS1 and VSG showed their relationships with T. evansi in other hosts from different countries. Conclusions: In our peer knowledge, it is the first time to report a research-based prevalence of trypanosomosis in the camels of Taif governorate, Makkah province, KSA. (© 2022. The Author(s).) |
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| Grant Information: | TURSP-2020/299 Taif University |
| Contributed Indexing: | Keywords: ITS1; KSA; Phylogeny; Rotat 1.2 VSG; Taif governorate; Trypanosomosis |
| Entry Date(s): | Date Created: 20220119 Date Completed: 20220303 Latest Revision: 20220303 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC8764778 |
| DOI: | 10.1186/s12917-022-03148-0 |
| PMID: | 35042521 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1746-6148 |
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| DOI: | 10.1186/s12917-022-03148-0 |