Academic Journal
Ralstonia solanacearum Type III Effector RipJ Triggers Bacterial Wilt Resistance in Solanum pimpinellifolium.
| Τίτλος: | Ralstonia solanacearum Type III Effector RipJ Triggers Bacterial Wilt Resistance in Solanum pimpinellifolium. |
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| Συγγραφείς: | Pandey A; Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea., Moon H; Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea., Choi S; Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea., Yoon H; Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea., Prokchorchik M; Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.; Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Germany., Jayaraman J; New Zealand Institute for Plant & Food Research Limited (PFR), Mt Albert Auckland 1025, New Zealand., Sujeevan R; Division of Biological Sciences and Research Institute for Basic Science, Wonkwang University, Iksan 54538, Republic of Korea., Kang YM; Division of Horticulture Industry, Wonkwang University, Iksan 554438, Republic of Korea., McCann HC; Institute of Advanced Studies, Massey University, Auckland 0745, New Zealand.; Max Planck Institute for Developmental Biology, Tübingen, Germany., Segonzac C; Department of Plant Science, Plant Genome and Breeding Institute, Agricultural Life Science Research Institute, Seoul National University, 08826, Seoul, Republic of Korea.; Plant Immunity Research Center, Seoul National University, 08826, Seoul, Republic of Korea.; Department of Agriculture, Forestry and Bioresources, Seoul National University, 08826, Seoul, Republic of Korea., Kim CM; Division of Horticulture Industry, Wonkwang University, Iksan 554438, Republic of Korea., Park SJ; Division of Biological Sciences and Research Institute for Basic Science, Wonkwang University, Iksan 54538, Republic of Korea., Sohn KH; Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.; School of Interdisciplinary Biosciences and Bioengineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea. |
| Πηγή: | Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2021 Aug; Vol. 34 (8), pp. 962-972. Date of Electronic Publication: 2021 Sep 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: APS Press Country of Publication: United States NLM ID: 9107902 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0894-0282 (Print) Linking ISSN: 08940282 NLM ISO Abbreviation: Mol Plant Microbe Interact Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: St. Paul, MN : APS Press, [c1987- |
| Ιατρικοί όροι (MeSH): | Ralstonia solanacearum* , Solanum*, Bacterial Proteins/genetics ; Disease Resistance ; Plant Diseases |
| Περίληψη: | Ralstonia solanacearum causes bacterial wilt disease in solanaceous crops. Identification of avirulence type III-secreted effectors recognized by specific disease resistance proteins in host plant species is an important step toward developing durable resistance in crops. In the present study, we show that R. solanacearum effector RipJ functions as an avirulence determinant in Solanum pimpinellifolium LA2093. In all, 10 candidate avirulence effectors were shortlisted based on the effector repertoire comparison between avirulent Pe_9 and virulent Pe_1 strains. Infection assays with transgenic strain Pe_1 individually carrying a candidate avirulence effector from Pe_9 revealed that only RipJ elicits strong bacterial wilt resistance in S. pimpinellifolium LA2093. Furthermore, we identified that several RipJ natural variants do not induce bacterial wilt resistance in S. pimpinellifolium LA2093. RipJ belongs to the YopJ family of acetyltransferases. Our sequence analysis indicated the presence of partially conserved putative catalytic residues. Interestingly, the conserved amino acid residues in the acetyltransferase catalytic triad are not required for effector-triggered immunity. In addition, we show that RipJ does not autoacetylate its lysine residues. Our study reports the identification of the first R. solanacearum avirulence protein that triggers bacterial wilt resistance in tomato. We expect that our discovery of RipJ as an avirulence protein will accelerate the development of bacterial wilt-resistant tomato varieties in the future.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| Contributed Indexing: | Keywords: Ralstonia solanacearum; Solanum pimpinellifolium; avirulence; bacterial wilt resistance; plant immunity; plant-pathogenic bacteria |
| Substance Nomenclature: | 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20210421 Date Completed: 20211111 Latest Revision: 20211111 |
| Update Code: | 20260130 |
| DOI: | 10.1094/MPMI-09-20-0256-R |
| PMID: | 33881922 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 0894-0282 |
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| DOI: | 10.1094/MPMI-09-20-0256-R |