Academic Journal
Failure Mechanisms in DNA Self-Assembly: Barriers to Single-Fold Yield.
| Τίτλος: | Failure Mechanisms in DNA Self-Assembly: Barriers to Single-Fold Yield. |
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| Συγγραφείς: | Majikes JM; National Institute of Standards and Technology, Gaithersburg, Maryland 20899-6203, United States., Patrone PN; National Institute of Standards and Technology, Gaithersburg, Maryland 20899-6203, United States., Kearsley AJ; National Institute of Standards and Technology, Gaithersburg, Maryland 20899-6203, United States., Zwolak M; National Institute of Standards and Technology, Gaithersburg, Maryland 20899-6203, United States., Liddle JA; National Institute of Standards and Technology, Gaithersburg, Maryland 20899-6203, United States. |
| Πηγή: | ACS nano [ACS Nano] 2021 Feb 23; Vol. 15 (2), pp. 3284-3294. Date of Electronic Publication: 2021 Feb 10. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington D.C. : American Chemical Society |
| Ιατρικοί όροι (MeSH): | DNA* , Nanostructures*, Nanotechnology ; Nucleic Acid Conformation |
| Περίληψη: | Understanding the folding process of DNA origami is a critical stepping stone to the broader implementation of nucleic acid nanofabrication technology but is notably nontrivial. Origami are formed by several hundred cooperative hybridization events-folds-between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples. Individual events are difficult to detect. Here, we present a real-time probe of the unit operation of origami assembly, a single fold, across the scaffold as a function of hybridization domain separation-fold distance-and staple/scaffold ratio. This approach to the folding problem elucidates a predicted but previously unobserved blocked state that acts as a limit on yield for single folds, which may manifest as a barrier in whole origami assembly. |
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| Grant Information: | 9999-NIST United States ImNIST Intramural NIST DOC |
| Contributed Indexing: | Keywords: DNA origami; hybridization; melting; nanostructures; self-assembly yield; thermodynamic modeling |
| Substance Nomenclature: | 9007-49-2 (DNA) |
| Entry Date(s): | Date Created: 20210210 Date Completed: 20210514 Latest Revision: 20250530 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC11005093 |
| DOI: | 10.1021/acsnano.0c10114 |
| PMID: | 33565312 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1936-086X |
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| DOI: | 10.1021/acsnano.0c10114 |