Academic Journal
Identification of RipAZ1 as an avirulence determinant of Ralstonia solanacearum in Solanum americanum.
| Τίτλος: | Identification of RipAZ1 as an avirulence determinant of Ralstonia solanacearum in Solanum americanum. |
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| Συγγραφείς: | Moon H; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea., Pandey A; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea., Yoon H; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea., Choi S; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea., Jeon H; Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul, Republic of Korea.; Plant Immunity Research Center, Seoul National University, Seoul, Republic of Korea., Prokchorchik M; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea., Jung G; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea., Witek K; The Sainsbury Laboratory, University of East Anglia, Norwich, UK., Valls M; Department of Genetics, University of Barcelona, Barcelona, Spain.; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Spain., McCann HC; New Zealand Institute of Advanced Studies, Massey University, Auckland, New Zealand.; Max Planck Institute for Developmental Biology, Tübingen, Germany., Kim MS; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.; Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Republic of Korea., Jones JDG; The Sainsbury Laboratory, University of East Anglia, Norwich, UK., Segonzac C; Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul, Republic of Korea.; Plant Immunity Research Center, Seoul National University, Seoul, Republic of Korea.; Department of Plant Science, Plant Genomics and Breeding Institute, Agricultural Life Science Research Institute, Seoul National University, Seoul, Republic of Korea., Sohn KH; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.; School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea. |
| Πηγή: | Molecular plant pathology [Mol Plant Pathol] 2021 Mar; Vol. 22 (3), pp. 317-333. Date of Electronic Publication: 2021 Jan 03. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Blackwell Science in collaboration with the British Society of Plant Pathology Country of Publication: England NLM ID: 100954969 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1364-3703 (Electronic) Linking ISSN: 13643703 NLM ISO Abbreviation: Mol Plant Pathol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford, UK : Blackwell Science in collaboration with the British Society of Plant Pathology, c2000- |
| Ιατρικοί όροι (MeSH): | Bacterial Proteins/*metabolism , Disease Resistance/*genetics , Plant Diseases/*microbiology , Ralstonia solanacearum/*genetics , Solanum/*microbiology, Bacterial Proteins/genetics ; Plant Diseases/immunology ; Ralstonia solanacearum/pathogenicity ; Plant Immunity ; Plant Leaves ; Virulence |
| Περίληψη: | Ralstonia solanacearum causes bacterial wilt disease in many plant species. Type III-secreted effectors (T3Es) play crucial roles in bacterial pathogenesis. However, some T3Es are recognized by corresponding disease resistance proteins and activate plant immunity. In this study, we identified the R. solanacearum T3E protein RipAZ1 (Ralstonia injected protein AZ1) as an avirulence determinant in the black nightshade species Solanum americanum. Based on the S. americanum accession-specific avirulence phenotype of R. solanacearum strain Pe_26, 12 candidate avirulence T3Es were selected for further analysis. Among these candidates, only RipAZ1 induced a cell death response when transiently expressed in a bacterial wilt-resistant S. americanum accession. Furthermore, loss of ripAZ1 in the avirulent R. solanacearum strain Pe_26 resulted in acquired virulence. Our analysis of the natural sequence and functional variation of RipAZ1 demonstrated that the naturally occurring C-terminal truncation results in loss of RipAZ1-triggered cell death. We also show that the 213 amino acid central region of RipAZ1 is sufficient to induce cell death in S. americanum. Finally, we show that RipAZ1 may activate defence in host cell cytoplasm. Taken together, our data indicate that the nucleocytoplasmic T3E RipAZ1 confers R. solanacearum avirulence in S. americanum. Few avirulence genes are known in vascular bacterial phytopathogens and ripAZ1 is the first one in R. solanacearum that is recognized in black nightshades. This work thus opens the way for the identification of disease resistance genes responsible for the specific recognition of RipAZ1, which can be a source of resistance against the devastating bacterial wilt disease. (© 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.) |
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| Grant Information: | BB/M017834/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/P021646/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| Contributed Indexing: | Keywords: Ralstonia solanacearum; Solanum americanum; avirulence; bacterial wilt; effector; effector-triggered immunity |
| Substance Nomenclature: | 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20210103 Date Completed: 20210607 Latest Revision: 20250530 |
| Update Code: | 20260510 |
| PubMed Central ID: | PMC7865085 |
| DOI: | 10.1111/mpp.13030 |
| PMID: | 33389783 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1364-3703 |
|---|---|
| DOI: | 10.1111/mpp.13030 |