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Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation.

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Title: Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation.
Authors: Chen XC; Multi-Organ Transplant Program, University Health Network., Sekhon M; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto., Ma XZ; Multi-Organ Transplant Program, University Health Network., Manuel J; Multi-Organ Transplant Program, University Health Network., Chung S; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto., He E; Multi-Organ Transplant Program, University Health Network., Bartczak A; Multi-Organ Transplant Program, University Health Network., Fischer S; Department of Laboratory Medicine and Pathobiology, University of Toronto., Thoeni C; Department of Laboratory Medicine and Pathobiology, University of Toronto., Oldani G; Multi-Organ Transplant Program, University Health Network., Perciani CT; Multi-Organ Transplant Program, University Health Network., MacParland S; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto; Department of Laboratory Medicine and Pathobiology, University of Toronto; s.macparland@utoronto.ca., McGilvray I; Multi-Organ Transplant Program, University Health Network; ian.macgilvray@uhn.ca.
Source: Journal of visualized experiments : JoVE [J Vis Exp] 2020 Nov 07 (165). Date of Electronic Publication: 2020 Nov 07.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
Language: English
Journal Info: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE
Imprint Name(s): Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]-
MeSH Terms: Graft Rejection/*prevention & control , Hepatic Artery/*surgery , Liver Diseases/*surgery , Liver Transplantation/*veterinary , Portal Vein/*surgery , Postoperative Complications/*prevention & control, Liver Transplantation/methods ; Animals ; Male ; Rats ; Rats, Inbred Lew
Abstract: The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation of human liver transplantation due to the absence of arterial reconnection. Described here is a modified transplantation procedure that includes the incorporation of hepatic artery (HA) reconnection, leading to a marked improvement in transplant outcomes. With a mean anhepatic time of 12 min and 14 s, HA reconnection results in improved perfusion of the transplanted liver and an increase in long-term recipient survival from 37.5% to 88.2%. This protocol includes the use of 3D-printed cuffs and holders to connect the portal vein and infrahepatic inferior vena cava. It can be implemented for studying multiple aspects of liver transplantation, from immune response and infection to technical aspects of the procedure. By incorporating a simple and practical method for arterial reconnection using a microvascular technique, this modified rat OLT protocol closely mimics aspects of human liver transplantation and will serve as a valuable and clinically relevant research model.
Entry Date(s): Date Created: 20201123 Date Completed: 20201229 Latest Revision: 20201229
Update Code: 20260130
DOI: 10.3791/60628
PMID: 33226024
Database: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 33226024
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Chen+XC%22">Chen XC</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22Sekhon+M%22">Sekhon M</searchLink>; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto.<br /><searchLink fieldCode="AU" term="%22Ma+XZ%22">Ma XZ</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22Manuel+J%22">Manuel J</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22Chung+S%22">Chung S</searchLink>; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto.<br /><searchLink fieldCode="AU" term="%22He+E%22">He E</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22Bartczak+A%22">Bartczak A</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22Fischer+S%22">Fischer S</searchLink>; Department of Laboratory Medicine and Pathobiology, University of Toronto.<br /><searchLink fieldCode="AU" term="%22Thoeni+C%22">Thoeni C</searchLink>; Department of Laboratory Medicine and Pathobiology, University of Toronto.<br /><searchLink fieldCode="AU" term="%22Oldani+G%22">Oldani G</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22Perciani+CT%22">Perciani CT</searchLink>; Multi-Organ Transplant Program, University Health Network.<br /><searchLink fieldCode="AU" term="%22MacParland+S%22">MacParland S</searchLink>; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto; Department of Laboratory Medicine and Pathobiology, University of Toronto; s.macparland@utoronto.ca.<br /><searchLink fieldCode="AU" term="%22McGilvray+I%22">McGilvray I</searchLink>; Multi-Organ Transplant Program, University Health Network; ian.macgilvray@uhn.ca.
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  Data: <searchLink fieldCode="JN" term="%22101313252%22">Journal of visualized experiments : JoVE</searchLink> [J Vis Exp] 2020 Nov 07 (165). <i>Date of Electronic Publication: </i>2020 Nov 07.
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  Data: Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
– Name: Language
  Label: Language
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  Data: English
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  Label: Journal Info
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22MYJoVE+Corporation%22">MYJoVE Corporation </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101313252 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1940-087X (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%221940087X%22">1940087X </searchLink><i>NLM ISO Abbreviation: </i>J Vis Exp <i>Subsets: </i>MEDLINE
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  Data: <i>Original Publication</i>: [Boston, Mass. : MYJoVE Corporation, 2006]-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Graft+Rejection%22">Graft Rejection</searchLink>/<searchLink fieldCode="MM" term="%22Graft+Rejection+prevention+%26+control%22">*prevention & control</searchLink> <br /><searchLink fieldCode="MM" term="%22Hepatic+Artery%22">Hepatic Artery</searchLink>/<searchLink fieldCode="MM" term="%22Hepatic+Artery+surgery%22">*surgery</searchLink> <br /><searchLink fieldCode="MM" term="%22Liver+Diseases%22">Liver Diseases</searchLink>/<searchLink fieldCode="MM" term="%22Liver+Diseases+surgery%22">*surgery</searchLink> <br /><searchLink fieldCode="MM" term="%22Liver+Transplantation%22">Liver Transplantation</searchLink>/<searchLink fieldCode="MM" term="%22Liver+Transplantation+veterinary%22">*veterinary</searchLink> <br /><searchLink fieldCode="MM" term="%22Portal+Vein%22">Portal Vein</searchLink>/<searchLink fieldCode="MM" term="%22Portal+Vein+surgery%22">*surgery</searchLink> <br /><searchLink fieldCode="MM" term="%22Postoperative+Complications%22">Postoperative Complications</searchLink>/<searchLink fieldCode="MM" term="%22Postoperative+Complications+prevention+%26+control%22">*prevention & control</searchLink><br /><searchLink fieldCode="MH" term="%22Liver+Transplantation%22">Liver Transplantation</searchLink>/<searchLink fieldCode="MH" term="%22Liver+Transplantation+methods%22">methods</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Male%22">Male</searchLink> ; <searchLink fieldCode="MH" term="%22Rats%22">Rats</searchLink> ; <searchLink fieldCode="MH" term="%22Rats%2C+Inbred+Lew%22">Rats, Inbred Lew</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation of human liver transplantation due to the absence of arterial reconnection. Described here is a modified transplantation procedure that includes the incorporation of hepatic artery (HA) reconnection, leading to a marked improvement in transplant outcomes. With a mean anhepatic time of 12 min and 14 s, HA reconnection results in improved perfusion of the transplanted liver and an increase in long-term recipient survival from 37.5% to 88.2%. This protocol includes the use of 3D-printed cuffs and holders to connect the portal vein and infrahepatic inferior vena cava. It can be implemented for studying multiple aspects of liver transplantation, from immune response and infection to technical aspects of the procedure. By incorporating a simple and practical method for arterial reconnection using a microvascular technique, this modified rat OLT protocol closely mimics aspects of human liver transplantation and will serve as a valuable and clinically relevant research model.
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20201123 <i>Date Completed: </i>20201229 <i>Latest Revision: </i>20201229
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– Name: DOI
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  Data: 10.3791/60628
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  Label: PMID
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  Data: 33226024
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=33226024
RecordInfo BibRecord:
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      – Type: doi
        Value: 10.3791/60628
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Liver Transplantation methods
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Male
        Type: general
      – SubjectFull: Rats
        Type: general
      – SubjectFull: Rats, Inbred Lew
        Type: general
      – SubjectFull: Graft Rejection prevention & control
        Type: general
      – SubjectFull: Hepatic Artery surgery
        Type: general
      – SubjectFull: Liver Diseases surgery
        Type: general
      – SubjectFull: Liver Transplantation veterinary
        Type: general
      – SubjectFull: Portal Vein surgery
        Type: general
      – SubjectFull: Postoperative Complications prevention & control
        Type: general
    Titles:
      – TitleFull: Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation.
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            NameFull: Chen XC
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            – D: 07
              M: 11
              Text: 2020 Nov 07
              Type: published
              Y: 2020
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              Value: 165
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