Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation. |
| Συγγραφείς: |
Chen XC; Multi-Organ Transplant Program, University Health Network., Sekhon M; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto., Ma XZ; Multi-Organ Transplant Program, University Health Network., Manuel J; Multi-Organ Transplant Program, University Health Network., Chung S; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto., He E; Multi-Organ Transplant Program, University Health Network., Bartczak A; Multi-Organ Transplant Program, University Health Network., Fischer S; Department of Laboratory Medicine and Pathobiology, University of Toronto., Thoeni C; Department of Laboratory Medicine and Pathobiology, University of Toronto., Oldani G; Multi-Organ Transplant Program, University Health Network., Perciani CT; Multi-Organ Transplant Program, University Health Network., MacParland S; Multi-Organ Transplant Program, University Health Network; Department of Immunology, University of Toronto; Department of Laboratory Medicine and Pathobiology, University of Toronto; s.macparland@utoronto.ca., McGilvray I; Multi-Organ Transplant Program, University Health Network; ian.macgilvray@uhn.ca. |
| Πηγή: |
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Nov 07 (165). Date of Electronic Publication: 2020 Nov 07. |
| Τύπος έκδοσης: |
Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media |
| Γλώσσα: |
English |
| Στοιχεία περιοδικού: |
Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| Imprint Name(s): |
Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| Ιατρικοί όροι (MeSH): |
Graft Rejection/*prevention & control , Hepatic Artery/*surgery , Liver Diseases/*surgery , Liver Transplantation/*veterinary , Portal Vein/*surgery , Postoperative Complications/*prevention & control, Liver Transplantation/methods ; Animals ; Male ; Rats ; Rats, Inbred Lew |
| Περίληψη: |
The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation of human liver transplantation due to the absence of arterial reconnection. Described here is a modified transplantation procedure that includes the incorporation of hepatic artery (HA) reconnection, leading to a marked improvement in transplant outcomes. With a mean anhepatic time of 12 min and 14 s, HA reconnection results in improved perfusion of the transplanted liver and an increase in long-term recipient survival from 37.5% to 88.2%. This protocol includes the use of 3D-printed cuffs and holders to connect the portal vein and infrahepatic inferior vena cava. It can be implemented for studying multiple aspects of liver transplantation, from immune response and infection to technical aspects of the procedure. By incorporating a simple and practical method for arterial reconnection using a microvascular technique, this modified rat OLT protocol closely mimics aspects of human liver transplantation and will serve as a valuable and clinically relevant research model. |
| Entry Date(s): |
Date Created: 20201123 Date Completed: 20201229 Latest Revision: 20201229 |
| Update Code: |
20260130 |
| DOI: |
10.3791/60628 |
| PMID: |
33226024 |
| Βάση Δεδομένων: |
MEDLINE |