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Revealing thermodynamics of DNA origami folding via affine transformations.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Revealing thermodynamics of DNA origami folding via affine transformations.
Συγγραφείς: Majikes JM; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA., Patrone PN; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA., Schiffels D; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA., Zwolak M; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA., Kearsley AJ; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA., Forry SP; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA., Liddle JA; National Institute of Standards and Technology, Gaithersburg, MD 20899-6203, USA.
Πηγή: Nucleic acids research [Nucleic Acids Res] 2020 Jun 04; Vol. 48 (10), pp. 5268-5280.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
Imprint Name(s): Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
Ιατρικοί όροι (MeSH): Thermodynamics*, DNA/*chemistry, Nanostructures/chemistry ; Calorimetry, Differential Scanning ; Entropy ; Fluorescence Resonance Energy Transfer ; Fluorescent Dyes ; Nucleic Acid Conformation ; Nucleic Acid Denaturation
Περίληψη: Structural DNA nanotechnology, as exemplified by DNA origami, has enabled the design and construction of molecularly-precise objects for a myriad of applications. However, limitations in imaging, and other characterization approaches, make a quantitative understanding of the folding process challenging. Such an understanding is necessary to determine the origins of structural defects, which constrain the practical use of these nanostructures. Here, we combine careful fluorescent reporter design with a novel affine transformation technique that, together, permit the rigorous measurement of folding thermodynamics. This method removes sources of systematic uncertainty and resolves problems with typical background-correction schemes. This in turn allows us to examine entropic corrections associated with folding and potential secondary and tertiary structure of the scaffold. Our approach also highlights the importance of heat-capacity changes during DNA melting. In addition to yielding insight into DNA origami folding, it is well-suited to probing fundamental processes in related self-assembling systems.
(Published by Oxford University Press on behalf of Nucleic Acids Research 2020.)
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Substance Nomenclature: 0 (Fluorescent Dyes)
9007-49-2 (DNA)
Entry Date(s): Date Created: 20200430 Date Completed: 20200817 Latest Revision: 20231111
Update Code: 20260130
PubMed Central ID: PMC7261180
DOI: 10.1093/nar/gkaa283
PMID: 32347943
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1362-4962
DOI:10.1093/nar/gkaa283