Academic Journal
Neutralizing antibodies for Helicobacter pylori urease inhibit bacterial colonization in the murine stomach in vivo.
| Τίτλος: | Neutralizing antibodies for Helicobacter pylori urease inhibit bacterial colonization in the murine stomach in vivo. |
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| Συγγραφείς: | Takeshita H; Department of Microbiology and Immunology, Nippon Medical School.; Department of Pediatrics, Nippon Medical School., Watanabe E; Department of Microbiology and Immunology, Nippon Medical School., Norose Y; Department of Microbiology and Immunology, Nippon Medical School., Ito Y; Department of Pediatrics, Nippon Medical School., Takahashi H; Department of Microbiology and Immunology, Nippon Medical School. |
| Πηγή: | Biomedical research (Tokyo, Japan) [Biomed Res] 2019; Vol. 40 (2), pp. 87-95. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Biomedical Research Foundation Country of Publication: Japan NLM ID: 8100317 Publication Model: Print Cited Medium: Internet ISSN: 1880-313X (Electronic) Linking ISSN: 03886107 NLM ISO Abbreviation: Biomed Res |
| Imprint Name(s): | Original Publication: Tokyo : Biomedical Research Foundation, 1980- |
| Ιατρικοί όροι (MeSH): | Antibodies, Bacterial/*biosynthesis , Antibodies, Neutralizing/*biosynthesis , Bacterial Proteins/*immunology , Helicobacter Infections/*prevention & control , Immunization/*methods , Peptides/*immunology , Urease/*immunology, Bacterial Proteins/genetics ; Epitopes/chemistry ; Epitopes/immunology ; Gastric Mucosa/drug effects ; Gastric Mucosa/immunology ; Gastric Mucosa/microbiology ; Helicobacter Infections/genetics ; Helicobacter Infections/immunology ; Helicobacter Infections/microbiology ; Helicobacter pylori/drug effects ; Helicobacter pylori/growth & development ; Helicobacter pylori/immunology ; Immune Sera/administration & dosage ; Peptides/administration & dosage ; Peptides/chemical synthesis ; Stomach/drug effects ; Stomach/immunology ; Stomach/microbiology ; Toll-Like Receptor 2/genetics ; Toll-Like Receptor 2/immunology ; Urease/deficiency ; Urease/genetics ; Amino Acid Sequence ; Animals ; Female ; Gene Deletion ; Gene Expression ; Mice ; Mice, Inbred BALB C |
| Περίληψη: | Helicobacter pylori (H. pylori) urease is a key protein for persistent infection of the bacteria in the stomach. Although H. pylori generally induce anti-H. pylori-specific antibodies (Abs), these Abs do not usually work for eradication or prevention of the H. pylori infection. In our previous study, we identified a linear epitope composed of 19-mer peptides termed UB-33, CHHLDKSIKEDVQFADSRI, within the large subunit of H. pylori urease. Anti-UB-33-specific Abs neutralized the enzymatic activity of H. pylori urease in vitro. In the present study, we evaluated the effect of immunization of BALB/c mice with H. pylori UB-33 peptide. After confirming the production of anti-UB-33-specific Abs, mice were challenged orally with H. pylori Sydney Strain-1 (SS-1). Mice producing anti-UB-33-specific Abs were not infected with SS-1, and the amount of SS-1 isolate in their stomach was significantly reduced. Also, the urease-negative mutant of H. pylori, HPP1801, did not colonize in the stomach, indicating that H. pylori urease was a critical element for infection of H. pylori in the gastric mucosa. Moreover, mice producing UB-33-specific Abs apparently suppressed H. pylori infection in the stomach where anti-UB-33 Abs were secreted in the gastric juice, indicating that H. pylori colonization was inhibited in the presence of anti-UB-33 Abs. In addition, the neutralization activity of sera from mice immunized with purified urease was less potent than that in the sera from mice immunized with UB-33. Furthermore, the recognition of epitope UB-33 was mediated through Toll-like receptor 2 (TLR2) on the B-1 cells using TLR2-knockout BALB/c mice in vivo. These results indicate that liner peptide UB-33 should be used for immunization to induce neutralizing Abs instead of purified H. pylori urease to prevent H. pylori infection and their colonization in the stomach. |
| Substance Nomenclature: | 0 (Antibodies, Bacterial) 0 (Antibodies, Neutralizing) 0 (Bacterial Proteins) 0 (Epitopes) 0 (Immune Sera) 0 (Peptides) 0 (Tlr2 protein, mouse) 0 (Toll-Like Receptor 2) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20190416 Date Completed: 20190802 Latest Revision: 20190802 |
| Update Code: | 20260130 |
| DOI: | 10.2220/biomedres.40.87 |
| PMID: | 30982804 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1880-313X |
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| DOI: | 10.2220/biomedres.40.87 |