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Subnanometer localization accuracy in widefield optical microscopy.

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Τίτλος: Subnanometer localization accuracy in widefield optical microscopy.
Συγγραφείς: Copeland CR; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.; 2Maryland NanoCenter, University of Maryland, College Park, MD 20742 USA., Geist J; 3Engineering Physics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA., McGray CD; 3Engineering Physics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA., Aksyuk VA; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA., Liddle JA; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA., Ilic BR; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA., Stavis SM; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.
Πηγή: Light, science & applications [Light Sci Appl] 2018 Jul 11; Vol. 7, pp. 31. Date of Electronic Publication: 2018 Jul 11 (Print Publication: 2018).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer Nature Country of Publication: England NLM ID: 101610753 Publication Model: eCollection Cited Medium: Internet ISSN: 2047-7538 (Electronic) Linking ISSN: 20477538 NLM ISO Abbreviation: Light Sci Appl Subsets: PubMed not MEDLINE
Imprint Name(s): Original Publication: [London] : Springer Nature, [2012]-
Περίληψη: The common assumption that precision is the limit of accuracy in localization microscopy and the typical absence of comprehensive calibration of optical microscopes lead to a widespread issue-overconfidence in measurement results with nanoscale statistical uncertainties that can be invalid due to microscale systematic errors. In this article, we report a comprehensive solution to this underappreciated problem. We develop arrays of subresolution apertures into the first reference materials that enable localization errors approaching the atomic scale across a submillimeter field. We present novel methods for calibrating our microscope system using aperture arrays and develop aberration corrections that reach the precision limit of our reference materials. We correct and register localization data from multiple colors and test different sources of light emission with equal accuracy, indicating the general applicability of our reference materials and calibration methods. In a first application of our new measurement capability, we introduce the concept of critical-dimension localization microscopy, facilitating tests of nanofabrication processes and quality control of aperture arrays. In a second application, we apply these stable reference materials to answer open questions about the apparent instability of fluorescent nanoparticles that commonly serve as fiducial markers. Our study establishes a foundation for subnanometer localization accuracy in widefield optical microscopy.
Competing Interests: The authors declare that they have no conflict of interest.
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Nat Methods. 2010 May;7(5):377-81. (PMID: 20364147)
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J Cell Biol. 2009 Jun 29;185(7):1135-48. (PMID: 19564400)
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Opt Express. 2017 May 15;25(10):11701-11716. (PMID: 28788730)
Phys Chem Chem Phys. 2014 Oct 21;16(39):21586-94. (PMID: 25189193)
Opt Express. 2012 May 21;20(11):12177-83. (PMID: 22714205)
PLoS One. 2015 Nov 23;10(11):e0142949. (PMID: 26600467)
Angew Chem Int Ed Engl. 2009;48(47):8870-3. (PMID: 19830751)
J Microsc. 2017 Jun;266(3):298-306. (PMID: 28328030)
Biophys J. 2005 Mar;88(3):2126-36. (PMID: 15596511)
Optica. 2015 Nov 20;2(11):985-993. (PMID: 26973863)
Nat Photonics. 2016 Nov;10(11):705-708. (PMID: 27818707)
Opt Express. 2015 Sep 7;23(18):23887-98. (PMID: 26368482)
J Res Natl Inst Stand Technol. 2016 Oct 19;121:464-475. (PMID: 34434635)
Nat Methods. 2012 Jun 10;9(7):724-6. (PMID: 22688415)
J Microsc. 2016 Sep;263(3):238-49. (PMID: 26970565)
Microsyst Nanoeng. 2016;2:16055. (PMID: 27840694)
Opt Express. 2013 Dec 2;21(24):29462-87. (PMID: 24514501)
Nat Methods. 2014 Mar;11(3):267-79. (PMID: 24577277)
Appl Opt. 2007 Jan 20;46(3):421-7. (PMID: 17228390)
Biophys J. 2017 May 23;112(10):2196-2208. (PMID: 28538156)
Anal Chem. 2015 Jun 16;87(12):6419-24. (PMID: 26011175)
Science. 2003 Jun 27;300(5628):2061-5. (PMID: 12791999)
Opt Express. 2009 Dec 21;17(26):23352-73. (PMID: 20052043)
J Microsc. 2004 Oct;216(Pt 1):49-51. (PMID: 15369482)
Appl Phys Rev. 2016 Mar;3(1):. (PMID: 27213022)
Entry Date(s): Date Created: 20190307 Latest Revision: 20230928
Update Code: 20260130
PubMed Central ID: PMC6107003
DOI: 10.1038/s41377-018-0031-z
PMID: 30839614
Βάση Δεδομένων: MEDLINE
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  Data: Subnanometer localization accuracy in widefield optical microscopy.
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  Data: <searchLink fieldCode="AU" term="%22Copeland+CR%22">Copeland CR</searchLink>; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.; 2Maryland NanoCenter, University of Maryland, College Park, MD 20742 USA.<br /><searchLink fieldCode="AU" term="%22Geist+J%22">Geist J</searchLink>; 3Engineering Physics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.<br /><searchLink fieldCode="AU" term="%22McGray+CD%22">McGray CD</searchLink>; 3Engineering Physics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.<br /><searchLink fieldCode="AU" term="%22Aksyuk+VA%22">Aksyuk VA</searchLink>; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.<br /><searchLink fieldCode="AU" term="%22Liddle+JA%22">Liddle JA</searchLink>; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.<br /><searchLink fieldCode="AU" term="%22Ilic+BR%22">Ilic BR</searchLink>; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.<br /><searchLink fieldCode="AU" term="%22Stavis+SM%22">Stavis SM</searchLink>; 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 USA.
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  Data: The common assumption that precision is the limit of accuracy in localization microscopy and the typical absence of comprehensive calibration of optical microscopes lead to a widespread issue-overconfidence in measurement results with nanoscale statistical uncertainties that can be invalid due to microscale systematic errors. In this article, we report a comprehensive solution to this underappreciated problem. We develop arrays of subresolution apertures into the first reference materials that enable localization errors approaching the atomic scale across a submillimeter field. We present novel methods for calibrating our microscope system using aperture arrays and develop aberration corrections that reach the precision limit of our reference materials. We correct and register localization data from multiple colors and test different sources of light emission with equal accuracy, indicating the general applicability of our reference materials and calibration methods. In a first application of our new measurement capability, we introduce the concept of critical-dimension localization microscopy, facilitating tests of nanofabrication processes and quality control of aperture arrays. In a second application, we apply these stable reference materials to answer open questions about the apparent instability of fluorescent nanoparticles that commonly serve as fiducial markers. Our study establishes a foundation for subnanometer localization accuracy in widefield optical microscopy.
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  Data: The authors declare that they have no conflict of interest.
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  Data: Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):E2812-20. (PMID: <searchLink fieldCode="PM" term="%2223821748%22">23821748)</searchLink><br />Nat Methods. 2014 Mar;11(3):253-66. (PMID: <searchLink fieldCode="PM" term="%2224577276%22">24577276)</searchLink><br />Nat Commun. 2015 Oct 16;6:8621. (PMID: <searchLink fieldCode="PM" term="%2226509412%22">26509412)</searchLink><br />Nat Methods. 2013 Jan;10(1):60-3. (PMID: <searchLink fieldCode="PM" term="%2223223154%22">23223154)</searchLink><br />Nat Commun. 2017 May 03;8:15115. (PMID: <searchLink fieldCode="PM" term="%2228466844%22">28466844)</searchLink><br />Science. 2012 Jan 13;335(6065):221-5. (PMID: <searchLink fieldCode="PM" term="%2222157083%22">22157083)</searchLink><br />Sci Rep. 2016 Jul 06;6:28680. (PMID: <searchLink fieldCode="PM" term="%2227381055%22">27381055)</searchLink><br />J Microsc. 2004 Oct;216(Pt 1):32-48. (PMID: <searchLink fieldCode="PM" term="%2215369481%22">15369481)</searchLink><br />Nature. 2012 Jul 5;487(7405):64-9. (PMID: <searchLink fieldCode="PM" term="%2222763440%22">22763440)</searchLink><br />Nat Commun. 2016 Jul 25;7:12290. (PMID: <searchLink fieldCode="PM" term="%2227452975%22">27452975)</searchLink><br />Nature. 2010 Jul 29;466(7306):647-51. (PMID: <searchLink fieldCode="PM" term="%2220613725%22">20613725)</searchLink><br />Nat Commun. 2015 Jan 07;6:5885. (PMID: <searchLink fieldCode="PM" term="%2225565216%22">25565216)</searchLink><br />J Opt Soc Am A Opt Image Sci Vis. 2001 Sep;18(9):2072-8. (PMID: <searchLink fieldCode="PM" term="%2211551037%22">11551037)</searchLink><br />Opt Express. 2013 Jul 1;21(13):15131-43. (PMID: <searchLink fieldCode="PM" term="%2223842300%22">23842300)</searchLink><br />Nat Methods. 2010 May;7(5):377-81. (PMID: <searchLink fieldCode="PM" term="%2220364147%22">20364147)</searchLink><br />Nat Methods. 2013 Jul;10(7):653-8. (PMID: <searchLink fieldCode="PM" term="%2223708387%22">23708387)</searchLink><br />J Cell Biol. 2009 Jun 29;185(7):1135-48. (PMID: <searchLink fieldCode="PM" term="%2219564400%22">19564400)</searchLink><br />Biophys J. 2002 May;82(5):2775-83. (PMID: <searchLink fieldCode="PM" term="%2211964263%22">11964263)</searchLink><br />Opt Express. 2017 May 15;25(10):11701-11716. (PMID: <searchLink fieldCode="PM" term="%2228788730%22">28788730)</searchLink><br />Phys Chem Chem Phys. 2014 Oct 21;16(39):21586-94. (PMID: <searchLink fieldCode="PM" term="%2225189193%22">25189193)</searchLink><br />Opt Express. 2012 May 21;20(11):12177-83. (PMID: <searchLink fieldCode="PM" term="%2222714205%22">22714205)</searchLink><br />PLoS One. 2015 Nov 23;10(11):e0142949. (PMID: <searchLink fieldCode="PM" term="%2226600467%22">26600467)</searchLink><br />Angew Chem Int Ed Engl. 2009;48(47):8870-3. (PMID: <searchLink fieldCode="PM" term="%2219830751%22">19830751)</searchLink><br />J Microsc. 2017 Jun;266(3):298-306. (PMID: <searchLink fieldCode="PM" term="%2228328030%22">28328030)</searchLink><br />Biophys J. 2005 Mar;88(3):2126-36. (PMID: <searchLink fieldCode="PM" term="%2215596511%22">15596511)</searchLink><br />Optica. 2015 Nov 20;2(11):985-993. (PMID: <searchLink fieldCode="PM" term="%2226973863%22">26973863)</searchLink><br />Nat Photonics. 2016 Nov;10(11):705-708. (PMID: <searchLink fieldCode="PM" term="%2227818707%22">27818707)</searchLink><br />Opt Express. 2015 Sep 7;23(18):23887-98. (PMID: <searchLink fieldCode="PM" term="%2226368482%22">26368482)</searchLink><br />J Res Natl Inst Stand Technol. 2016 Oct 19;121:464-475. (PMID: <searchLink fieldCode="PM" term="%2234434635%22">34434635)</searchLink><br />Nat Methods. 2012 Jun 10;9(7):724-6. (PMID: <searchLink fieldCode="PM" term="%2222688415%22">22688415)</searchLink><br />J Microsc. 2016 Sep;263(3):238-49. (PMID: <searchLink fieldCode="PM" term="%2226970565%22">26970565)</searchLink><br />Microsyst Nanoeng. 2016;2:16055. (PMID: <searchLink fieldCode="PM" term="%2227840694%22">27840694)</searchLink><br />Opt Express. 2013 Dec 2;21(24):29462-87. (PMID: <searchLink fieldCode="PM" term="%2224514501%22">24514501)</searchLink><br />Nat Methods. 2014 Mar;11(3):267-79. (PMID: <searchLink fieldCode="PM" term="%2224577277%22">24577277)</searchLink><br />Appl Opt. 2007 Jan 20;46(3):421-7. (PMID: <searchLink fieldCode="PM" term="%2217228390%22">17228390)</searchLink><br />Biophys J. 2017 May 23;112(10):2196-2208. (PMID: <searchLink fieldCode="PM" term="%2228538156%22">28538156)</searchLink><br />Anal Chem. 2015 Jun 16;87(12):6419-24. (PMID: <searchLink fieldCode="PM" term="%2226011175%22">26011175)</searchLink><br />Science. 2003 Jun 27;300(5628):2061-5. (PMID: <searchLink fieldCode="PM" term="%2212791999%22">12791999)</searchLink><br />Opt Express. 2009 Dec 21;17(26):23352-73. (PMID: <searchLink fieldCode="PM" term="%2220052043%22">20052043)</searchLink><br />J Microsc. 2004 Oct;216(Pt 1):49-51. (PMID: <searchLink fieldCode="PM" term="%2215369482%22">15369482)</searchLink><br />Appl Phys Rev. 2016 Mar;3(1):. (PMID: <searchLink fieldCode="PM" term="%2227213022%22">27213022)</searchLink>
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