Academic Journal
Intraperitoneal immunization with Urease loaded N-trimethyl Chitosan nanoparticles elicits high protection against Brucella melitensis and Brucella abortus infections.
| Τίτλος: | Intraperitoneal immunization with Urease loaded N-trimethyl Chitosan nanoparticles elicits high protection against Brucella melitensis and Brucella abortus infections. |
|---|---|
| Συγγραφείς: | Abkar M; Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: mortezaabkar@gmail.com., Fasihi-Ramandi M; Molecular Biology Research Center, System Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: fasihi.m@gmail.com., Kooshki H; Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: hmdkooshki@gmail.com., Lotfi AS; Department of Clinical Biochemistry, Faculty of Medical Sciences Tarbiat Modares University, Tehran, Iran. Electronic address: lotfi_ab@owa.modares.ac.ir. |
| Πηγή: | Immunology letters [Immunol Lett] 2018 Jul; Vol. 199, pp. 53-60. Date of Electronic Publication: 2018 Mar 13. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7910006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0542 (Electronic) Linking ISSN: 01652478 NLM ISO Abbreviation: Immunol Lett Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| Ιατρικοί όροι (MeSH): | Brucellosis*/drug therapy , Brucellosis*/immunology , Brucellosis*/prevention & control, Brucella Vaccine/*immunology , Chitosan/*therapeutic use , Nanoparticles/*therapeutic use , Urease/*immunology , Urease/*therapeutic use, Brucella abortus/immunology ; Brucella melitensis/immunology ; Immunoglobulin G/metabolism ; Animals ; Humans ; Injections, Intraperitoneal ; Mice ; Mice, Inbred BALB C ; Models, Animal ; Vaccination |
| Περίληψη: | Brucella (B) species are brucellosis causative agents, a worldwide zoonotic illness causing Malta fever in humans and abortion in domestic animals. In this work, we evaluated the vaccine potential of Trimethyl chitosan (TMC) nanoparticles formulation of Urease (TMC/Urease) against brucellosis. TMC/Urease nanoparticles and urease without any adjuvant were separately administered both orally and intraperitoneally. Intraperitoneal (i.p.) administration of urease alone as well as oral administration of both TMC/Urease nanoparticles and urease alone, elicited low titers of specific immunoglobulin G (IgG), while i.p. immunization with TMC/Urease nanoparticles induced high specific IgG production levels. As it was indicated by the cytokine assay and the antibody isotypes, i.p. immunization by urease alone, and TMC/Urease nanoparticles induced a mixed Th1-Th2 immune response, whereas oral administration of both urease alone and TMC/Urease nanoparticles induced a mixed Th1-Th17 immune response. In lymphocyte proliferation assay, spleen cells from i.p.-vaccinated mice with TMC/Urease nanoparticles showed a strong recall proliferative response. Vaccinated animals were challenged with virulent strains of B. melitensis and B. abortus. I.p. vaccination with TMC/Urease nanoparticles resulted in a high degree of protection. Altogether, our results indicated that TMC nanoparticles are a potent delivery system for i.p.-administered Brucella antigens. (Copyright © 2018. Published by Elsevier B.V.) |
| Contributed Indexing: | Keywords: Brucellosis; Nanoparticles; Trimethyl chitosan; Urease; Vaccine |
| Substance Nomenclature: | 0 (Brucella Vaccine) 0 (Immunoglobulin G) 0 (N-trimethyl chitosan chloride) 9012-76-4 (Chitosan) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20180318 Date Completed: 20190709 Latest Revision: 20190709 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.imlet.2018.03.004 |
| PMID: | 29548705 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-0542 |
|---|---|
| DOI: | 10.1016/j.imlet.2018.03.004 |