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Short-term evolution of Shiga toxin-producing Escherichia coli O157:H7 between two food-borne outbreaks.

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Τίτλος: Short-term evolution of Shiga toxin-producing Escherichia coli O157:H7 between two food-borne outbreaks.
Συγγραφείς: Cowley LA; 1​Gastrointestinal Bacterial Reference Unit, 61 Colindale Avenue, Public Health England, NW9 5EQ London, UK., Dallman TJ; 1​Gastrointestinal Bacterial Reference Unit, 61 Colindale Avenue, Public Health England, NW9 5EQ London, UK., Fitzgerald S; 2​Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9RG Roslin, UK., Irvine N; 3​Public Health Agency, 12-22 Linenhall St, BT2 8BS Belfast, Northern Ireland., Rooney PJ; 4​Microbiology Laboratory, Royal Victoria Hospital, BT12 6BA Belfast, Northern Ireland., McAteer SP; 2​Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9RG Roslin, UK., Day M; 1​Gastrointestinal Bacterial Reference Unit, 61 Colindale Avenue, Public Health England, NW9 5EQ London, UK., Perry NT; 1​Gastrointestinal Bacterial Reference Unit, 61 Colindale Avenue, Public Health England, NW9 5EQ London, UK., Bono JL; 5​U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166, USA., Jenkins C; 1​Gastrointestinal Bacterial Reference Unit, 61 Colindale Avenue, Public Health England, NW9 5EQ London, UK., Gally DL; 2​Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9RG Roslin, UK.
Πηγή: Microbial genomics [Microb Genom] 2016 Sep 08; Vol. 2 (9), pp. e000084. Date of Electronic Publication: 2016 Sep 08 (Print Publication: 2016).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Microbiology Society Country of Publication: England NLM ID: 101671820 Publication Model: eCollection Cited Medium: Print ISSN: 2057-5858 (Print) Linking ISSN: 20575858 NLM ISO Abbreviation: Microb Genom Subsets: MEDLINE
Imprint Name(s): Publication: Oct. 2015- : [London] : Microbiology Society
Original Publication: [London] : Society for General Microbiology, [2015]-
Ιατρικοί όροι (MeSH): Escherichia coli Infections/*microbiology , Escherichia coli O157/*genetics , Genome, Bacterial/*genetics, Escherichia coli Infections/epidemiology ; Escherichia coli Infections/transmission ; Plasmids/genetics ; Prophages/genetics ; Africa, Northern ; Disease Outbreaks ; Evolution, Molecular ; Humans ; Middle East
Περίληψη: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a public health threat and outbreaks occur worldwide. Here, we investigate genomic differences between related STEC O157:H7 that caused two outbreaks, eight weeks apart, at the same restaurant. Short-read genome sequencing divided the outbreak strains into two sub-clusters separated by only three single-nucleotide polymorphisms in the core genome while traditional typing identified them as separate phage types, PT8 and PT54. Isolates did not cluster with local strains but with those associated with foreign travel to the Middle East/North Africa. Combined long-read sequencing approaches and optical mapping revealed that the two outbreak strains had undergone significant microevolution in the accessory genome with prophage gain, loss and recombination. In addition, the PT54 sub-type had acquired a 240 kbp multi-drug resistance (MDR) IncHI2 plasmid responsible for the phage type switch. A PT54 isolate had a general fitness advantage over a PT8 isolate in rich medium, including an increased capacity to use specific amino acids and dipeptides as a nitrogen source. The second outbreak was considerably larger and there were multiple secondary cases indicative of effective human-to-human transmission. We speculate that MDR plasmid acquisition and prophage changes have adapted the PT54 strain for human infection and transmission. Our study shows the added insights provided by combining whole-genome sequencing approaches for outbreak investigations.
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Contributed Indexing: Keywords: Bioinformatics; Escherichia coli; Evolution; Prophage; Recombination
Entry Date(s): Date Created: 20170329 Date Completed: 20181101 Latest Revision: 20260127
Update Code: 20260130
PubMed Central ID: PMC5320650
DOI: 10.1099/mgen.0.000084
PMID: 28348875
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2057-5858
DOI:10.1099/mgen.0.000084