Academic Journal
Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells.
| Τίτλος: | Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells. |
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| Συγγραφείς: | Guan G; 2 Department of Otolaryngology, The Second Affiliated Hospital of Jilin University, Changchun, China., Li R; 3 Department of Urinary Surgery, The Second Affiliated Hospital of Jilin University, Changchun, China., Tang W; 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China., Liu T; 4 Department of Anesthesiology, The Second Affiliated Hospital of Jilin University, Changchun, China., Su Z; 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China., Wang Y; 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China., Tan J; 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China., Jiang S; 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China., Wang K; 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China. |
| Πηγή: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2017 Mar; Vol. 39 (3), pp. 1010428317691740. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: SAGE Publications Country of Publication: United States NLM ID: 8409922 Publication Model: Print Cited Medium: Internet ISSN: 1423-0380 (Electronic) Linking ISSN: 10104283 NLM ISO Abbreviation: Tumour Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2025- : [Thousand Oaks, CA] : SAGE Publications Original Publication: Tokyo, Japan : Saikon Pub. Co., c1984- |
| Ιατρικοί όροι (MeSH): | Adenocarcinoma/*metabolism , Adenocarcinoma/*pathology , Lung Neoplasms/*metabolism , Lung Neoplasms/*pathology , RNA-Binding Proteins/*biosynthesis, Adenocarcinoma/genetics ; Lung Neoplasms/genetics ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; RNA-Binding Proteins/genetics ; A549 Cells ; Adenocarcinoma of Lung ; Cell Line, Tumor ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Neoplasm Staging ; Oligonucleotide Array Sequence Analysis |
| Περίληψη: | This study assessed RNA-binding motif 10 expression in lung adenocarcinoma tissues and examined the role and mechanism of RNA-binding motif 10 in the regulation of lung adenocarcinoma malignancy. Lung adenocarcinoma and corresponding adjacent non-tumor lung tissues from 41 patients were subjected to reverse transcription-polymerase chain reaction and Western blot assessment to detect RNA-binding motif 10 expression. Recombinant lentivirus carrying RNA-binding motif 10 complementary DNA was used to infect lung adenocarcinoma cell lines, A549 and H1299 cells. Complementary DNA microarray was used to profile RNA-binding motif 10-regulated genes. Levels of RNA-binding motif 10 messenger RNA and protein were significantly lower in lung adenocarcinoma tissues than those in paired non-tumor tissues (p < 0.001). Reduced RNA-binding motif 10 expression was found to be associated with an advanced tumor stage. RNA-binding motif 10 overexpression inhibited viability and colony formation capacity of lung adenocarcinoma cell lines and induced cell-cycle arrest at G0/G1 phase in A549 cells and at S phase in H1299 cells. Complementary DNA microarray analysis identified 304 upregulated and 386 downregulated genes induced by RNA-binding motif 10 overexpression, which may be involved in cancer, focal adhesion, peroxisome proliferator-activated receptor-regulated gene pathway, cytokine-cytokine receptor interaction, mitogen-activated protein kinase signaling, complement and coagulation cascades, platelet amyloid precursor protein pathway, extracellular matrix-receptor interaction, and small cell lung cancer-related genes. Expression of FGF2, EGFR, WNT5A, NF-κB, and RAP1A was downregulated, whereas expression of AKT2, BIRC3, and JUN was upregulated. RNA-binding motif 10 messenger RNA and protein were reduced in lung adenocarcinoma tissues, and RNA-binding motif 10 overexpression inhibited lung adenocarcinoma cancer cell malignant behavior in vitro. Molecularly, RNA-binding motif 10 regulates many gene pathways involving in the tumor development or progression. |
| Contributed Indexing: | Keywords: RNA-binding motif 10; complementary DNA microarray; lung adenocarcinoma; tumor suppressor gene |
| Substance Nomenclature: | 0 (RBM10 protein, human) 0 (RNA, Messenger) 0 (RNA-Binding Proteins) |
| Entry Date(s): | Date Created: 20170329 Date Completed: 20170407 Latest Revision: 20190221 |
| Update Code: | 20260130 |
| DOI: | 10.1177/1010428317691740 |
| PMID: | 28347232 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1423-0380 |
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| DOI: | 10.1177/1010428317691740 |