Academic Journal
Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model.
| Τίτλος: | Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model. |
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| Συγγραφείς: | Fekrazad R; 1 Department of Periodontology, Dental Faculty, AJA University of Medical Sciences , Tehran, Iran ., Eslaminejad MB; 2 Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology , ACECR, Tehran, Iran ., Shayan AM; 3 Department of Orthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences , Tabriz, Iran ., Kalhori KA; 4 Oral & Maxillofacial Pathologist, Iranian Medical Laser Association, Tehran, Iran ., Abbas FM; 5 Department of Oral & Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences , Tehran, Iran ., Taghiyar L; 6 Department of Stem Cells and Developmental Biology, Cell Sciences Research Center, Royan Institute for Stem Cell Biology and Technology , ACECR, Tehran, Iran ., Sepehr Pedram M; 7 Department of Surgery & Radiology, Faculty of Veterinary Medicine, University of Tehran , Tehran, Iran ., Ghuchani MS; 8 Department of Orthodontics, Gorgan Faculty of Dentistry, Golestan University of Medical Sciences , Golestan, Iran . |
| Πηγή: | Photomedicine and laser surgery [Photomed Laser Surg] 2016 Nov; Vol. 34 (11), pp. 543-549. Date of Electronic Publication: 2016 Apr 08. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101222340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8550 (Electronic) Linking ISSN: 15495418 NLM ISO Abbreviation: Photomed Laser Surg Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-c2018 |
| Ιατρικοί όροι (MeSH): | Bone Marrow Transplantation/*methods , Cartilage, Articular/*radiation effects , Low-Level Light Therapy/*methods , Mesenchymal Stem Cells/*radiation effects, Bone Marrow Cells/radiation effects ; Cartilage, Articular/injuries ; Cartilage, Articular/pathology ; Knee Joint/pathology ; Knee Joint/radiation effects ; Regeneration/physiology ; Regeneration/radiation effects ; Analysis of Variance ; Animals ; Biopsy, Needle ; Cells, Cultured ; Disease Models, Animal ; Immunohistochemistry ; Male ; Rabbits ; Random Allocation ; Treatment Outcome |
| Περίληψη: | Objective: The aim of this study was to evaluate the effectiveness of the application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) with scaffold and low-level laser therapy (LLLT) on the repair of articular cartilage defects in rabbits. Background Data: For healing of the articular cartilage defects, although positive effects of BMSCs and LLLT have been demonstrated, their combination effect is still unknown; therefore, we investigated combining these two techniques has a synergistic effect. Materials and Methods: After bone marrow aspiration from 10 rabbits, BMSCs were isolated, cultured in monolayer, suspended on a type I collagen scaffold and then implanted onto a full-thickness osteochondral defect (4 mm in diameter), artificially made on the patellar groove of both knees in the same rabbits. Then a knee was selected randomly in each rabbit as the experimental group, and subjected to Ga-Al-As (810 nm) laser irradiation with energy density of 4 J/cm2 every other day for 3 weeks. As the control group, the other knee did not receive LLLT. After this period, animals were euthanized and osteochondral defects were evaluated by histomorphometric methods. Results: No significant difference in new cartilage formation and inflammation was found between the groups (p > 0.05). However, there was significantly more new bone formation in the experimental group (p < 0.05). Conclusions: In terms of our research, although better healing in osteochondral defects was seen when combining BMSCs and LLLT compared with the use of BMSCs alone, this improvement was predominantly caused by new bone formation rather than new cartilage formation. |
| Contributed Indexing: | Keywords: articular cartilage repair; bone marrow mesenchymal stem cells; low-level laser therapy; osteochondral defect; photobiomodulation; rabbit model |
| Entry Date(s): | Date Created: 20160409 Date Completed: 20170414 Latest Revision: 20220330 |
| Update Code: | 20260130 |
| DOI: | 10.1089/pho.2015.4028 |
| PMID: | 27058019 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1557-8550 |
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| DOI: | 10.1089/pho.2015.4028 |