Academic Journal
Whole Genome Sequencing for Public Health Surveillance of Shiga Toxin-Producing Escherichia coli Other than Serogroup O157.
| Τίτλος: | Whole Genome Sequencing for Public Health Surveillance of Shiga Toxin-Producing Escherichia coli Other than Serogroup O157. |
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| Συγγραφείς: | Chattaway MA; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Dallman TJ; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Gentle A; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Wright MJ; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Long SE; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Ashton PM; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Perry NT; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK., Jenkins C; Gastrointestinal Bacteria Reference Unit, Public Health England London, UK. |
| Πηγή: | Frontiers in microbiology [Front Microbiol] 2016 Mar 03; Vol. 7, pp. 258. Date of Electronic Publication: 2016 Mar 03 (Print Publication: 2016). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Lausanne : Frontiers Research Foundation |
| Περίληψη: | Shiga toxin-producing Escherichia coli (STEC) are considered to be a significant threat to public health due to the severity of gastrointestinal symptoms associated with human infection. In England STEC O157 is the most commonly detected STEC serogroup, however, the implementation of PCR at local hospital laboratories has resulted in an increase in the detection of non-O157 STEC. The aim of this study was to evaluate the use of whole genome sequencing (WGS) for routine public health surveillance of non-O157 STEC by comparing this approach to phenotypic serotyping and PCR for subtyping the stx-encoding genes. Of the 102 isolates where phenotypic and genotypic serotyping could be compared, 98 gave fully concordant results. The most common non-O157 STEC serogroups detected were O146 (22) and O26 (18). All but one of the 38 isolates that could not be phenotypically serotyped (designated O unidentifiable or O rough) were serotyped using the WGS data. Of the 73 isolates where a flagella type was available by traditional phenotypic typing, all results matched the H-type derived from the WGS data. Of the 140 sequenced non-O157 isolates, 52 (37.1%) harboured stx1 only, 42 (30.0%) had stx2 only, 46 (32.9%) carried stx1 and stx2. Of these, stx subtyping PCR results were available for 131 isolates and 121 of these had concordant results with the stx subtype derived from the WGS data. Of the 10 discordant results, non-specific primer binding during PCR amplification, due to the similarity of the stx2 subtype gene sequences was the most likely cause. The results of this study showed WGS provided a reliable and robust one-step process for characterization of STEC. Deriving the full serotype from WGS data in real time has enabled us to report a higher level of strain discrimination while stx subtyping provides data on the pathogenic potential of each isolate, enabling us to predict clinical outcome of each case and to monitor the emergence of hyper-virulent strains. |
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| Contributed Indexing: | Keywords: Shiga Toxin-producing Escherichia coli; serotyping; stx subtyping; whole genome sequencing |
| Entry Date(s): | Date Created: 20160315 Date Completed: 20160314 Latest Revision: 20201001 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC4776118 |
| DOI: | 10.3389/fmicb.2016.00258 |
| PMID: | 26973632 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1664-302X |
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| DOI: | 10.3389/fmicb.2016.00258 |