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Human urinary exosomes as innate immune effectors.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Human urinary exosomes as innate immune effectors.
Συγγραφείς: Hiemstra TF; Department of Medicine, Cambridge Centre for Proteome Research and Cambridge Systems Biology Centre, Department of Biochemistry., Charles PD; Cambridge Centre for Proteome Research and Cambridge Systems Biology Centre, Department of Biochemistry., Gracia T; Department of Medical Genetics, and., Hester SS; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Gatto L; Cambridge Centre for Proteome Research and Cambridge Systems Biology Centre, Department of Biochemistry., Al-Lamki R; Department of Medicine., Floto RA; Department of Medicine., Su Y; Department of Medical Genetics, and., Skepper JN; Multi-Imaging Centre, Department of Anatomy, University of Cambridge, Cambridge, United Kingdom; and., Lilley KS; Cambridge Centre for Proteome Research and Cambridge Systems Biology Centre, Department of Biochemistry, ksl23@cam.ac.uk fek1000@cam.ac.uk., Karet Frankl FE; Department of Medical Genetics, and ksl23@cam.ac.uk fek1000@cam.ac.uk.
Πηγή: Journal of the American Society of Nephrology : JASN [J Am Soc Nephrol] 2014 Sep; Vol. 25 (9), pp. 2017-27. Date of Electronic Publication: 2014 Apr 03.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wolters Kluwer Health, on behalf of the American Society of Nephrology Country of Publication: United States NLM ID: 9013836 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1533-3450 (Electronic) Linking ISSN: 10466673 NLM ISO Abbreviation: J Am Soc Nephrol Subsets: MEDLINE
Imprint Name(s): Publication: 2023- : Hagerstown, MD : Wolters Kluwer Health, on behalf of the American Society of Nephrology
Original Publication: Baltimore, MD : Williams & Wilkins, c1990-
Ιατρικοί όροι (MeSH): Immunity, Innate*, Exosomes/*immunology , Urinary Tract/*immunology, Biomarkers/urine ; Exosomes/ultrastructure ; Proteome/immunology ; Urinary Tract/microbiology ; Uropathogenic Escherichia coli/growth & development ; Uropathogenic Escherichia coli/immunology ; Adult ; Female ; Humans ; Male ; Microscopy, Immunoelectron ; Young Adult
Περίληψη: Exosomes are small extracellular vesicles, approximately 50 nm in diameter, derived from the endocytic pathway and released by a variety of cell types. Recent data indicate a spectrum of exosomal functions, including RNA transfer, antigen presentation, modulation of apoptosis, and shedding of obsolete protein. Exosomes derived from all nephron segments are also present in human urine, where their function is unknown. Although one report suggested in vitro uptake of exosomes by renal cortical collecting duct cells, most studies of human urinary exosomes have focused on biomarker discovery rather than exosome function. Here, we report results from in-depth proteomic analyses and EM showing that normal human urinary exosomes are significantly enriched for innate immune proteins that include antimicrobial proteins and peptides and bacterial and viral receptors. Urinary exosomes, but not the prevalent soluble urinary protein uromodulin (Tamm-Horsfall protein), potently inhibited growth of pathogenic and commensal Escherichia coli and induced bacterial lysis. Bacterial killing depended on exosome structural integrity and occurred optimally at the acidic pH typical of urine from omnivorous humans. Thus, exosomes are innate immune effectors that contribute to host defense within the urinary tract.
(Copyright © 2014 by the American Society of Nephrology.)
Σχόλια: Comment in: J Am Soc Nephrol. 2014 Sep;25(9):1889-91. doi: 10.1681/ASN.2014020204.. (PMID: 24700877)
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Grant Information: 100140 United Kingdom Wellcome Trust; 088489 United Kingdom WT_ Wellcome Trust; United Kingdom MRC_ Medical Research Council
Substance Nomenclature: 0 (Biomarkers)
0 (Proteome)
Entry Date(s): Date Created: 20140405 Date Completed: 20141121 Latest Revision: 20211021
Update Code: 20260130
PubMed Central ID: PMC4147985
DOI: 10.1681/ASN.2013101066
PMID: 24700864
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1533-3450
DOI:10.1681/ASN.2013101066