Academic Journal
The serological response to Verocytotoxigenic Escherichia coli in patients with haemolytic uraemic syndrome.
| Τίτλος: | The serological response to Verocytotoxigenic Escherichia coli in patients with haemolytic uraemic syndrome. |
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| Συγγραφείς: | Chart H; Laboratory of Enteric Pathogens, Specialist and Reference Microbiology Division, Health Protection Agency, London, UK. henrik.chart@hpa.org.uk, Perry NT |
| Πηγή: | Letters in applied microbiology [Lett Appl Microbiol] 2004; Vol. 38 (5), pp. 351-4. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print Cited Medium: Print ISSN: 0266-8254 (Print) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2023- : Oxford : Oxford University Press Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985- |
| Ιατρικοί όροι (MeSH): | Antibodies, Bacterial/*blood , Escherichia coli/*immunology , Hemolytic-Uremic Syndrome/*microbiology , Shiga Toxins/*metabolism, Escherichia coli/metabolism ; Escherichia coli Infections/immunology ; Escherichia coli Infections/microbiology ; Escherichia coli O157/immunology ; Hemolytic-Uremic Syndrome/immunology ; Lipopolysaccharides/immunology ; Adolescent ; Child ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoblotting ; Male ; Serotyping |
| Περίληψη: | Aims: To screen sera from 80 patients with clinical haemolytic uraemic syndrome (HUS) and serum antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157, for antibodies to Verocytotoxin-producing Escherichia coli (VTEC) belonging to serogroups O5, O26, O104, O111, O128, O145, O153 and O165. Methods and Results: Sera were screened by an LPS-based ELISA and SDS-PAGE/immunoblotting. None of the 80 sera contained antibodies binding to long-chain LPS of any of the LPS types employed; however, nine sera contained antibodies binding to R3 LPS-core epitopes. Conclusions: The presence of patients' serum antibodies to the LPS of E. coli O157, in the absence of antibodies to the LPS of a range of other VTEC, demonstrated that cases of HUS may be caused by strains of O157 VTEC alone and that concurrent infection with multiple strains of VTEC is not a prerequisite for cases of HUS. Significance and Impact of the Study: Antibodies to long-chain LPS of VTEC other than O157 were not detected, and so there was no evidence of infection with VTEC belonging to more than one serogroup. The results of immunoassays such as ELISAs and micro-agglutinations must take into consideration antibodies binding to R3 epitopes located on LPS-core. |
| Substance Nomenclature: | 0 (Antibodies, Bacterial) 0 (Lipopolysaccharides) 0 (Shiga Toxins) |
| Entry Date(s): | Date Created: 20040403 Date Completed: 20040628 Latest Revision: 20041117 |
| Update Code: | 20260130 |
| DOI: | 10.1111/j.1472-765X.2004.01499.x |
| PMID: | 15059202 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 0266-8254 |
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| DOI: | 10.1111/j.1472-765X.2004.01499.x |