Academic Journal
Two substrate binding sites in ascorbate peroxidase: the role of arginine 172.
| Τίτλος: | Two substrate binding sites in ascorbate peroxidase: the role of arginine 172. |
|---|---|
| Συγγραφείς: | Bursey EH; Departments of Molecular Biology & Biochemistry and Physiology & Biophysics and the Program in Macromolecular Structure, University of California, Irvine 92697-3900, USA., Poulos TL |
| Πηγή: | Biochemistry [Biochemistry] 2000 Jun 27; Vol. 39 (25), pp. 7374-9. |
| Τύπος έκδοσης: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370623 Publication Model: Print Cited Medium: Print ISSN: 0006-2960 (Print) Linking ISSN: 00062960 NLM ISO Abbreviation: Biochemistry Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Chemical Society. |
| Ιατρικοί όροι (MeSH): | Arginine/*metabolism , Peroxidases/*metabolism, Guaiacol/metabolism ; Peroxidases/chemistry ; Peroxidases/genetics ; Ascorbate Peroxidases ; Kinetics ; Models, Molecular ; Mutagenesis ; Protein Conformation ; Substrate Specificity |
| Περίληψη: | Site-directed mutagenesis has been used to probe the role of Arg172 in ascorbate utilization by ascorbate peroxidase. Arg172 was changed to lysine, glutamine, and asparagine. Although each of these variants retains the ability to utilize guaiacol as a reductant, they exhibit large decreases in their steady-state rates of ascorbate utilization. Spectroscopic, steady-state, and transient-state experiments indicate that these variant proteins are capable of reacting with hydrogen peroxide to form Compound I, but their ability to oxidize ascorbate to form Compound II, and subsequently the resting state, is severely impeded. Results are presented which highlight the importance of Arg172, and a model is proposed to explain its role in ascorbate utilization. |
| Grant Information: | GM42614 United States GM NIGMS NIH HHS |
| Substance Nomenclature: | 6JKA7MAH9C (Guaiacol) 94ZLA3W45F (Arginine) EC 1.11.1.- (Peroxidases) EC 1.11.1.11 (Ascorbate Peroxidases) |
| Entry Date(s): | Date Created: 20000620 Date Completed: 20000804 Latest Revision: 20190613 |
| Update Code: | 20260130 |
| DOI: | 10.1021/bi000446s |
| PMID: | 10858284 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 0006-2960 |
|---|---|
| DOI: | 10.1021/bi000446s |